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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
12/08/2009 |
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Data da última atualização: |
26/06/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARRARA, V. S.; AMATO, A. A.; NEVES, F. A. R.; BAZOTTE, R. B.; MANDARINO, J. M. G.; NAKAMURA, C. V.; FILHO, B. P. D.; CORTEZ, D. A. G. |
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Afiliação: |
UEM; UnB; UnB; UEM; JOSÉ MARCOS GONTIJO MANDARINO, CNPSO; UEM; UEM; UEM. |
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Título: |
Effects of a methanolic fraction of soybean seeds on the transcriptional activity of peroxisome proliferator-activated receptors (PPAR). |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 42, n. 6, p. 545-550, June 2009. |
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Idioma: |
Inglês |
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Conteúdo: |
Since the anti-inflammatory, antidiabetic and hypolipidemic effects of soy isoflavones may be mediated by activation of peroxisome proliferator-activated receptors (PPAR), the present study investigated whether the methanolic fractions obtained from soybean seeds (E1) and soybean seed coats with hypocotyls (E2) could influence PPAR?, PPAR? and PPAR?/? transcriptional activity. The isoflavones from E1 and E2 were quantified by HPLC analysis. E1 and E2 were rich in isoflavones (daidzin, glycitin, genistin, malonyldaidzin, malonylglycitin, malonylgenistin, daidzein, glycitein, and genistein). Moreover, E1 and E2 showed no evidence of genetically modified material containing the gene CP4 EPSPS. To investigate PPAR transcriptional activity, human promonocytic U-937 cells were treated with E1 and E2 (200, 400, 800, and 1600 ?g/mL), positive controls or vehicle. Data are reported as fold-activation of the luciferase reporter driven by the PPAR-responsive element. Doseresponse analysis revealed that E1 and E2 induced the transcriptional activity of PPAR? (P < 0.001), with activation comparable to that obtained with 0.1 mM bezafibrate (positive control) at 1600 ?g/mL (4-fold) and 800 ?g/mL (9-fold), respectively. In addition, dose-response analysis revealed that E1 and E2 activated PPAR?/? (P < 0.05), and the activation at 800 ?g/mL (4- and 9-fold, respectively) was comparable to that of 0.1 mM bezafibrate (positive control). However, no effect on PPAR? was observed. Activation of PPAR? is consistent with the lipid-lowering activity of soy isoflavones in vivo, but further studies are needed to determine the physiological significance of PPAR?/? activation. MenosSince the anti-inflammatory, antidiabetic and hypolipidemic effects of soy isoflavones may be mediated by activation of peroxisome proliferator-activated receptors (PPAR), the present study investigated whether the methanolic fractions obtained from soybean seeds (E1) and soybean seed coats with hypocotyls (E2) could influence PPAR?, PPAR? and PPAR?/? transcriptional activity. The isoflavones from E1 and E2 were quantified by HPLC analysis. E1 and E2 were rich in isoflavones (daidzin, glycitin, genistin, malonyldaidzin, malonylglycitin, malonylgenistin, daidzein, glycitein, and genistein). Moreover, E1 and E2 showed no evidence of genetically modified material containing the gene CP4 EPSPS. To investigate PPAR transcriptional activity, human promonocytic U-937 cells were treated with E1 and E2 (200, 400, 800, and 1600 ?g/mL), positive controls or vehicle. Data are reported as fold-activation of the luciferase reporter driven by the PPAR-responsive element. Doseresponse analysis revealed that E1 and E2 induced the transcriptional activity of PPAR? (P < 0.001), with activation comparable to that obtained with 0.1 mM bezafibrate (positive control) at 1600 ?g/mL (4-fold) and 800 ?g/mL (9-fold), respectively. In addition, dose-response analysis revealed that E1 and E2 activated PPAR?/? (P < 0.05), and the activation at 800 ?g/mL (4- and 9-fold, respectively) was comparable to that of 0.1 mM bezafibrate (positive control). However, no effect on PPAR? was observed. Activation of PP... Mostrar Tudo |
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Palavras-Chave: |
Isoflavona. |
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Thesagro: |
Bioquímica; Nutrição Humana; Soja. |
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Thesaurus Nal: |
Soybeans. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02529naa a2200265 a 4500
001 1471379
005 2024-06-26
008 2009 bl uuuu u00u1 u #d
100 1 $aCARRARA, V. S.
245 $aEffects of a methanolic fraction of soybean seeds on the transcriptional activity of peroxisome proliferator-activated receptors (PPAR).$h[electronic resource]
260 $c2009
520 $aSince the anti-inflammatory, antidiabetic and hypolipidemic effects of soy isoflavones may be mediated by activation of peroxisome proliferator-activated receptors (PPAR), the present study investigated whether the methanolic fractions obtained from soybean seeds (E1) and soybean seed coats with hypocotyls (E2) could influence PPAR?, PPAR? and PPAR?/? transcriptional activity. The isoflavones from E1 and E2 were quantified by HPLC analysis. E1 and E2 were rich in isoflavones (daidzin, glycitin, genistin, malonyldaidzin, malonylglycitin, malonylgenistin, daidzein, glycitein, and genistein). Moreover, E1 and E2 showed no evidence of genetically modified material containing the gene CP4 EPSPS. To investigate PPAR transcriptional activity, human promonocytic U-937 cells were treated with E1 and E2 (200, 400, 800, and 1600 ?g/mL), positive controls or vehicle. Data are reported as fold-activation of the luciferase reporter driven by the PPAR-responsive element. Doseresponse analysis revealed that E1 and E2 induced the transcriptional activity of PPAR? (P < 0.001), with activation comparable to that obtained with 0.1 mM bezafibrate (positive control) at 1600 ?g/mL (4-fold) and 800 ?g/mL (9-fold), respectively. In addition, dose-response analysis revealed that E1 and E2 activated PPAR?/? (P < 0.05), and the activation at 800 ?g/mL (4- and 9-fold, respectively) was comparable to that of 0.1 mM bezafibrate (positive control). However, no effect on PPAR? was observed. Activation of PPAR? is consistent with the lipid-lowering activity of soy isoflavones in vivo, but further studies are needed to determine the physiological significance of PPAR?/? activation.
650 $aSoybeans
650 $aBioquímica
650 $aNutrição Humana
650 $aSoja
653 $aIsoflavona
700 1 $aAMATO, A. A.
700 1 $aNEVES, F. A. R.
700 1 $aBAZOTTE, R. B.
700 1 $aMANDARINO, J. M. G.
700 1 $aNAKAMURA, C. V.
700 1 $aFILHO, B. P. D.
700 1 $aCORTEZ, D. A. G.
773 $tBrazilian Journal of Medical and Biological Research, Ribeirão Preto$gv. 42, n. 6, p. 545-550, June 2009.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 32 | |
| 4. |  | ARAÚJO, E. R.; RESENDE, R. S.; LIMA, M. F. Crop protection. In: RABINOWITCH, H. D.; THOMAS, B. (ed.). Edible alliums: botany production and uses. Wallingfors: Cabi International, 2023. p.224-239.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Hortaliças. |
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| 6. | | SCHENK, M. A. M.; MENDONCA, C. L.; MADRUGA, C. R.; KOHAYAGAWA, A.; ARAUJO, E. R. de. Avaliacao clinico-laboratorial de bovinos nelores infectados experimentalmente com Trypanosoma vivax. In: SEMINARIO BRASILEIRO DE PARASITOLOGIA VETERINARIA, 11.; SEMINARIO DE PARASITOLOGIA VETERINARIA DOS PAISES DO MERCOSUL, 2.; SIMPOSIO DE CONTROLE INTEGRADO DE PARASITOS DE BOVINOS, 1., 1999, Salvador. Anais... Ilheus: Colegio Brasileiro de Parasitologia Veterinaria, [1999?]. p.207. CNPGC. Resumo TL-PT-014.| Biblioteca(s): Embrapa Gado de Corte. |
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| 8. | | ARAÚJO, E. R.; QUEZADO-DUVAL, A. M.; PEREIRA, R. C.; CAFÉ-FILHO, A. C. Avaliação de competitividade entre espécies de Xanthomonas causadoras da mancha bacteriana do tomateiro. Tropical Plant Pathology, Brasília, DF, v. 34, p. S 09, ago. 2009. Suplemento. Palestra apresentada no 42. Congresso Brasileiro de Fitopatologia, Rio de Janeiro.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| 11. | | ARAÚJO, E. R.; PEREIRA, R. C.; QUEZADO-DUVAL, A. M.; FERREIRA, M. A. S. V. Sensibilidade in vitro de isolados de Xanthomonas perforans a cobre e estreptomicina. Tropical plant Pathology, Brasília, DF, v. 34, p. S94, ago. 2009. Suplemento. Resumo 342. Trabalho apresentado no 42. Congresso Brasileiro de Fitopatologia, Rio de Janeiro.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| 16. | | ARAÚJO, E. R.; COSTA, J. R.; QUEZADO-DUVAL, A. M.; FERREIRA, M. A. S. V. Molecular detection of the Xanthomonas species complex causing tomato bacterial spot. Tropical Plant Pathology, Brasília, DF, v. 36, p. 132, 2011. 1 CD-ROM. Suplemento. Resumo 132. Edição dos resumos do 44º Congresso Brasileiro de Fitopatologia, 2011, Bento Gonçalves.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| 17. | | PEREIRA, R. C.; ARAÚJO, E. R.; FERREIRA, M. A. S. V.; QUEZADO-DUVAL, A. M. Occurrence of Xanthomonas species causing bacterial spot in fresh market tomato fields in Brazil. Acta Horticulturae, The Hague, n. 914, p. 61-64, Nov. 2011. Trabalho apresentado no III International Symposium on Tomato Diseases, Ischia, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Hortaliças. |
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| 18. | | COSTA, J. R.; ARAÚJO, E. R.; PONTES, N. C.; QUEZADO-DUVAL, A. M. Ocorrência de Xanthomonas perforans e X. gardneri em plantas infestantes em lavouras de tomate. Tropical Plant Pathology, Brasília, DF, v. 36, p. 131, 2011. 1 CD-ROM. Suplemento. Resumo 1024. Edição dos resumos do 44º Congresso Brasileiro de Fitopatologia, 2011, Bento Gonçalves.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| Registros recuperados : 32 | |
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