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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo; Embrapa Solos. |
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Data corrente: |
14/03/2000 |
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Data da última atualização: |
25/08/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, V. M. de; COUTINHO, H. L. da C.; SOBRAL, B. W. S.; GUIMARAES, C. T.; ELSAS, J. D. van; MANFIO, G. P. |
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Afiliação: |
V. M. DE OLIVEIRA, FUNDAÇÃO ANDRÉ TOSELLO; HEITOR LUIZ DA COSTA COUTINHO, CNPS; B. W. S. SOBRAL, NATIONAL CENTER FOR GENOME RESOURCES; C. T. GUIMARÃES, NATIONAL CENTER FOR GENOME RESOURCES; J. D. VAN ELSAS, RESEARCH INSTITUTE FOR PLANT PROTECTION; GILSON PAULO MANFIO, FUNDAÇÃO ANDRÉ TOSELLO. |
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Título: |
Discrimination of Rhizobium tropici and R. leguminosarum strains by PCR-specific amplification of 16S-23S rDNA spacer region fragments and denaturing gradient gel electrophoresis (DGGE). |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Letters in Applied Microbiology, v. 28, N. 2, p. 137-141, Feb. 1999. |
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DOI: |
https://doi.org/10.1046/j.1365-2672.1999.00480.x |
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Idioma: |
Inglês |
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Conteúdo: |
With the aim of detecting Rhizobium species directly in the environment, specific PCR primers for Rh, tropici and Rh, leguminosarum were designed on the basis of sequence analysis of 16S-23S rDNA spacer regions of several Rh. tropici, Rh. leguminosarum and Agrobacterium rhizogenes strains. Primer specificity was checked by comparison with available rDNA spacer sequences in databases, and by PCR using DNA from target and reference strains. Sequence polymorphisms of rDNA spacer fragments among strains of the same species were detected by denaturing gradient gel electrophoresis (DGGE). The specific PCR primers designed in the study could be applied to evaluate the diversity of Rh. tropici and Rh. leguminosarum by analysing the polymorphisms of 16S-23S spacer rDNA amplified from either whole-cell or soil-extracted DNA. |
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Palavras-Chave: |
Analysis; Database; Diversidade; Diversity; tropici. |
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Thesagro: |
Base de Dados; DNA; Eletroforese; Rhizobium; Solo. |
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Thesaurus Nal: |
electrophoresis; soil. |
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Categoria do assunto: |
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P Recursos Naturais, Ciências Ambientais e da Terra |
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Marc: |
LEADER 01876naa a2200337 a 4500
001 1335364
005 2023-08-25
008 1999 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1046/j.1365-2672.1999.00480.x$2DOI
100 1 $aOLIVEIRA, V. M. de
245 $aDiscrimination of Rhizobium tropici and R. leguminosarum strains by PCR-specific amplification of 16S-23S rDNA spacer region fragments and denaturing gradient gel electrophoresis (DGGE).$h[electronic resource]
260 $c1999
520 $aWith the aim of detecting Rhizobium species directly in the environment, specific PCR primers for Rh, tropici and Rh, leguminosarum were designed on the basis of sequence analysis of 16S-23S rDNA spacer regions of several Rh. tropici, Rh. leguminosarum and Agrobacterium rhizogenes strains. Primer specificity was checked by comparison with available rDNA spacer sequences in databases, and by PCR using DNA from target and reference strains. Sequence polymorphisms of rDNA spacer fragments among strains of the same species were detected by denaturing gradient gel electrophoresis (DGGE). The specific PCR primers designed in the study could be applied to evaluate the diversity of Rh. tropici and Rh. leguminosarum by analysing the polymorphisms of 16S-23S spacer rDNA amplified from either whole-cell or soil-extracted DNA.
650 $aelectrophoresis
650 $asoil
650 $aBase de Dados
650 $aDNA
650 $aEletroforese
650 $aRhizobium
650 $aSolo
653 $aAnalysis
653 $aDatabase
653 $aDiversidade
653 $aDiversity
653 $atropici
700 1 $aCOUTINHO, H. L. da C.
700 1 $aSOBRAL, B. W. S.
700 1 $aGUIMARAES, C. T.
700 1 $aELSAS, J. D. van
700 1 $aMANFIO, G. P.
773 $tLetters in Applied Microbiology$gv. 28, N. 2, p. 137-141, Feb. 1999.
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Registro original: |
Embrapa Solos (CNPS) |
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