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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
18/03/2004 |
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Data da última atualização: |
04/04/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BRONDANI, C.; BRONDANI, R. P. V.; RANGEL, P. H. N.; FERREIRA, M. E. |
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Afiliação: |
CLAUDIO BRONDANI, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF; PAULO HIDEO NAKANO RANGEL, CNPAF; MARCIO ELIAS FERREIRA, CENARGEN. |
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Título: |
Development and mapping of Oryza glumaepatula-derived microsatellite markers in the interspecific cross Oryza glumaepatula x O. sativa. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Hereditas, v. 134, n. 1, p. 59-71, Mar. 2001. |
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DOI: |
https://doi.org/10.1111/j.1601-5223.2001.00059.x |
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Idioma: |
Inglês |
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Conteúdo: |
Wild germplasm of domesticated crops is a source of genetic variation little utilized in breeding programs. Interspecific crosses can potentially uncover novel gene combinations that can be important for quantitative trait analysis. The combined use of wide crosses and genetic maps of chromosomal regions associated with quantitative traits can be used to broaden the genetic basis of rice breeding programs. Oryza glumaepatula is a diploid (AA genome) wild rice species native from South and Central America. A genetic map was constructed with 162 PCR-based markers (155 microsatellite and 7 STS markers) using a backcross population derived from the cross 0. glumaepatula, accession RS-16 from the Brazilian Amazon Region x 0. sativa BG-90-2, an elite rice inbred line. The map included 47 new SSR markers developed from an 0. glumaepatula genomic library enriched for AG/TC sequences. All SSR markers were able to amplify the 0. sativa genome, indicating a high degree of SSR flanking region conservation between 0. glumaepatula and 0. sativa species. The map covered 1500.4 cM, with an average of one marker every 10 cM. Despite some chromosomes being more densely mapped, the overall coverage was similar to other maps developed for rice. The advantage to construct a SSR-based map is to permit the combination of the speed of the PCR reaction, and the codominant nature of the SSR marker, facilitating the QTL analysis and marker assisted selection for rice breeding programs. |
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Palavras-Chave: |
Microsatellite; Oryza glumaepatula. |
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Thesagro: |
Arroz; Oryza Sativa. |
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Thesaurus Nal: |
rice. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02194naa a2200229 a 4500
001 1211977
005 2022-04-04
008 2001 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1111/j.1601-5223.2001.00059.x$2DOI
100 1 $aBRONDANI, C.
245 $aDevelopment and mapping of Oryza glumaepatula-derived microsatellite markers in the interspecific cross Oryza glumaepatula x O. sativa.
260 $c2001
520 $aWild germplasm of domesticated crops is a source of genetic variation little utilized in breeding programs. Interspecific crosses can potentially uncover novel gene combinations that can be important for quantitative trait analysis. The combined use of wide crosses and genetic maps of chromosomal regions associated with quantitative traits can be used to broaden the genetic basis of rice breeding programs. Oryza glumaepatula is a diploid (AA genome) wild rice species native from South and Central America. A genetic map was constructed with 162 PCR-based markers (155 microsatellite and 7 STS markers) using a backcross population derived from the cross 0. glumaepatula, accession RS-16 from the Brazilian Amazon Region x 0. sativa BG-90-2, an elite rice inbred line. The map included 47 new SSR markers developed from an 0. glumaepatula genomic library enriched for AG/TC sequences. All SSR markers were able to amplify the 0. sativa genome, indicating a high degree of SSR flanking region conservation between 0. glumaepatula and 0. sativa species. The map covered 1500.4 cM, with an average of one marker every 10 cM. Despite some chromosomes being more densely mapped, the overall coverage was similar to other maps developed for rice. The advantage to construct a SSR-based map is to permit the combination of the speed of the PCR reaction, and the codominant nature of the SSR marker, facilitating the QTL analysis and marker assisted selection for rice breeding programs.
650 $arice
650 $aArroz
650 $aOryza Sativa
653 $aMicrosatellite
653 $aOryza glumaepatula
700 1 $aBRONDANI, R. P. V.
700 1 $aRANGEL, P. H. N.
700 1 $aFERREIRA, M. E.
773 $tHereditas$gv. 134, n. 1, p. 59-71, Mar. 2001.
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