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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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Data corrente: |
24/02/2026 |
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Data da última atualização: |
24/02/2026 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LIMA, A. M. C.; FELIX, L. A.; WOSIACKI, S. R.; ALVES, F. S. F.; SOUZA, V. de; SOUSA, N. P. de; RODRIGUES, M. L. C.; BRANDAO, H. de M.; FERRANTE, M.; FACCIOLI-MARTINS, P. Y. |
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Afiliação: |
ANA MILENA CESAR LIMA; LARISSA ALEXSANDRA FELIX, UNIVERSIDADE FEDERAL DE LAVRAS; SHEILA REZLER WOSIACKI, UNIVERSIDADE ESTADUAL DE MARINGÁ; FRANCISCO SELMO FERNANDES ALVES, CNPC; VIVIANE DE SOUZA, CNPC; NIRLIR PLACIDO DE SOUSA, CENTRO UNIVERSITÁRIO INTA; MARIA LETICIA CARNEIRO RODRIGUES, CENTRO UNIVERSITÁRIO INTA; HUMBERTO DE MELLO BRANDAO, CNPGL; MARCOS FERRANTE, UNIVERSIDADE FEDERAL DE LAVRAS; PATRICIA YOSHIDA FACCIOLI MARTINS, CNPC. |
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Título: |
Time-kill curve analysis and pharmacodynamic modeling for in vitro evaluation of cloxacillin activity against Corynebacterium pseudotuberculosis. |
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Ano de publicação: |
2026 |
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Fonte/Imprenta: |
Small Ruminant Research, v. 258, 107732, May, 2026. |
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DOI: |
https://doi.org/10.1016/j.smallrumres.2026.107732 |
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Idioma: |
Inglês |
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Conteúdo: |
Current treatments for Corynebacterium pseudotuberculosis do not result in bacteriological cure and have low biosafety, requiring new therapeutic strategies. This study aimed to establish the tentative epidemiological cutoff (TECOFF) of cloxacillin for Corynebacterium pseudotuberculosis, assess bacterial response through time–kill curves, and identify PK/PD indices and targets describing the antimicrobial-pathogen interaction. The minimum inhibitory concentration (MIC) and time-kill curve were determined in cation-adjusted Miller-Hinton broth and the TECOFF was derived from MIC distribution. A sigmoid Emax model identified the PK/PD index and its targets (PDT) that best described the dose-response profile observed in the time-kill curves. PK/PD targets values were established for bactericidal (E = -3 log10) and eradication (E = -4 log10) activity. MICs for 35 isolates ranged from 4 to 16 µg/mL, with TECOFF of 16 µg/mL (CI 99.9 %). The PK/PD indices %T > MIC (R² = 0.85) and AUC/MIC (R² = 0.82) best described the relationship between pharmacokinetic and microbiological parameters, being strongly associated with the effect of cloxacillin against C. pseudotuberculosis. The PDT values were 45 % and 63 % for %T > MIC, and 31.79 and 45.5 for AUC48 h/MIC, for bactericidal and eradication activities, respectively. These findings support rational therapeutic protocols and highlight the need for validation in vivo under clinical conditions. |
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Palavras-Chave: |
Mal do caroço. |
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Thesagro: |
Antibiótico; Bactéria; Caprino; Corynebacterium Pseudotuberculosis; Doença Animal; Linfadenite Caseosa; Ovino; Ruminante. |
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Thesaurus Nal: |
Animal diseases; Caseous lymphadenitis; Goat diseases; Goats; Lymphadenitis; Ruminants; Sheep; Sheep diseases; Small ruminants. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02810naa a2200457 a 4500
001 2184604
005 2026-02-24
008 2026 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.smallrumres.2026.107732$2DOI
100 1 $aLIMA, A. M. C.
245 $aTime-kill curve analysis and pharmacodynamic modeling for in vitro evaluation of cloxacillin activity against Corynebacterium pseudotuberculosis.$h[electronic resource]
260 $c2026
520 $aCurrent treatments for Corynebacterium pseudotuberculosis do not result in bacteriological cure and have low biosafety, requiring new therapeutic strategies. This study aimed to establish the tentative epidemiological cutoff (TECOFF) of cloxacillin for Corynebacterium pseudotuberculosis, assess bacterial response through time–kill curves, and identify PK/PD indices and targets describing the antimicrobial-pathogen interaction. The minimum inhibitory concentration (MIC) and time-kill curve were determined in cation-adjusted Miller-Hinton broth and the TECOFF was derived from MIC distribution. A sigmoid Emax model identified the PK/PD index and its targets (PDT) that best described the dose-response profile observed in the time-kill curves. PK/PD targets values were established for bactericidal (E = -3 log10) and eradication (E = -4 log10) activity. MICs for 35 isolates ranged from 4 to 16 µg/mL, with TECOFF of 16 µg/mL (CI 99.9 %). The PK/PD indices %T > MIC (R² = 0.85) and AUC/MIC (R² = 0.82) best described the relationship between pharmacokinetic and microbiological parameters, being strongly associated with the effect of cloxacillin against C. pseudotuberculosis. The PDT values were 45 % and 63 % for %T > MIC, and 31.79 and 45.5 for AUC48 h/MIC, for bactericidal and eradication activities, respectively. These findings support rational therapeutic protocols and highlight the need for validation in vivo under clinical conditions.
650 $aAnimal diseases
650 $aCaseous lymphadenitis
650 $aGoat diseases
650 $aGoats
650 $aLymphadenitis
650 $aRuminants
650 $aSheep
650 $aSheep diseases
650 $aSmall ruminants
650 $aAntibiótico
650 $aBactéria
650 $aCaprino
650 $aCorynebacterium Pseudotuberculosis
650 $aDoença Animal
650 $aLinfadenite Caseosa
650 $aOvino
650 $aRuminante
653 $aMal do caroço
700 1 $aFELIX, L. A.
700 1 $aWOSIACKI, S. R.
700 1 $aALVES, F. S. F.
700 1 $aSOUZA, V. de
700 1 $aSOUSA, N. P. de
700 1 $aRODRIGUES, M. L. C.
700 1 $aBRANDAO, H. de M.
700 1 $aFERRANTE, M.
700 1 $aFACCIOLI-MARTINS, P. Y.
773 $tSmall Ruminant Research$gv. 258, 107732, May, 2026.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 1 | |
| 1. |  | CRUZ, P. J. R.; SILVA, D. V. da; LIMA, I. B. G. de; RAMALHO, I. O.; ALVES, B. J. R.; BODDEY, R. M.; HOMEM, B. G. C.; PAIVA, A. J.; CASAGRAMDE. D. R. Grazing intensity and seasonality regulate N cycling via litter and excreta in tropical grass–legume pastures. Nutrient Cycling in Agroecosystems, V. 132, N. 21, 2026,| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 1 | |
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