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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
20/08/2025 |
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Data da última atualização: |
20/08/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FERREIRA, J. P.; VIDAL, M. S.; BALDANI, J. I. |
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Afiliação: |
JÉSSICA P. FERREIRA, UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO; MARCIA SOARES VIDAL, CNPAB; JOSE IVO BALDANI, CNPAB. |
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Título: |
Exploring ACC deaminase-producing bacteria for drought stress mitigation in Brachiaria. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Frontiers Frontiers in Plant Science, v. 16, 18 august, 2025. |
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ISSN: |
1664-462X |
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DOI: |
10.3389/fpls.2025.1607697 |
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Idioma: |
Inglês |
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Conteúdo: |
Plant growth-promoting bacteria (PGPB) possessing 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity have the potential to enhance plant growth and development, particularly under adverse environmental conditions. This study aimed to identify bacterial strains with ACC deaminase activity able of mitigating the effects of water deficit stress and promoting the growth of Brachiaria genotypes. Bacterial strains isolated from Brachiaria genotypes were screened in vitro for ACC deaminase activity, and the presence of the acdS gene was confirmed via polymerase chain reaction (PCR) analysis. The bacterial isolates were screened for in vitro tolerance to water deficit stress, using 10% polyethylene glycol 8000 (PEG 8000) in association with B. ruziziensis and the effects of bacterial inoculation were assessed based on plant height and fresh biomass accumulation. Additionally, the association between endophytic bacterial strains and Brachiaria genotypes was evaluated using confocal laser microscope. The results showed that among the 213 strains tested, 32 demonstrate the ability to degrade ACC into α-ketobutyrate. ACC deaminase activity was detected in 17 strains, with values ranging from 1.98 to 102.52 μmol α-ketobutyrate mg ⁻¹ protein h ⁻¹ . The presence of the acd S gene was confirmed in nine strains. The strains NRB142 ( Paraburkholderia silvatlantica ), NRB223 ( Azospirillum melinis ), and BR11790 ( Herbaspirillum frisingense GSF30 T ) exhibited the most significant promotion of plant development in B . ruziziensis under water deficit stress mediated by 10% PEG 8000. Confocal microscopy analysis revealed the rhizospheric and inner root colonization of B . ruziziensis and B . brizantha cv. Paiaguás by the NRB142 mCherry-labeled strain. This study showed no predominance of a specific group of bacterial strains in terms of ACC deaminase activity. However, a subset of strains demonstrated the ability to colonize Brachiaria plants and mitigate the negative effects of water deficit stress. This study highlights the potential of ACC deaminase-producing bacteria in alleviating water deficit stress in Brachiaria plants supporting their use as a promising strategy for improving plant resilience under drought conditions. MenosPlant growth-promoting bacteria (PGPB) possessing 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity have the potential to enhance plant growth and development, particularly under adverse environmental conditions. This study aimed to identify bacterial strains with ACC deaminase activity able of mitigating the effects of water deficit stress and promoting the growth of Brachiaria genotypes. Bacterial strains isolated from Brachiaria genotypes were screened in vitro for ACC deaminase activity, and the presence of the acdS gene was confirmed via polymerase chain reaction (PCR) analysis. The bacterial isolates were screened for in vitro tolerance to water deficit stress, using 10% polyethylene glycol 8000 (PEG 8000) in association with B. ruziziensis and the effects of bacterial inoculation were assessed based on plant height and fresh biomass accumulation. Additionally, the association between endophytic bacterial strains and Brachiaria genotypes was evaluated using confocal laser microscope. The results showed that among the 213 strains tested, 32 demonstrate the ability to degrade ACC into α-ketobutyrate. ACC deaminase activity was detected in 17 strains, with values ranging from 1.98 to 102.52 μmol α-ketobutyrate mg ⁻¹ protein h ⁻¹ . The presence of the acd S gene was confirmed in nine strains. The strains NRB142 ( Paraburkholderia silvatlantica ), NRB223 ( Azospirillum melinis ), and BR11790 ( Herbaspirillum frisingense GSF30 T ) exhibited the most significant prom... Mostrar Tudo |
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Palavras-Chave: |
Ethylene stress; Plant colonization. |
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Thesaurus Nal: |
1-aminocyclopropane-1-carboxylic acid; Pastures; Plant growth-promoting rhizobacteria; Polyethylene glycol. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 03028naa a2200241 a 4500
001 2178163
005 2025-08-20
008 2025 bl uuuu u00u1 u #d
022 $a1664-462X
024 7 $a10.3389/fpls.2025.1607697$2DOI
100 1 $aFERREIRA, J. P.
245 $aExploring ACC deaminase-producing bacteria for drought stress mitigation in Brachiaria.$h[electronic resource]
260 $c2025
520 $aPlant growth-promoting bacteria (PGPB) possessing 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity have the potential to enhance plant growth and development, particularly under adverse environmental conditions. This study aimed to identify bacterial strains with ACC deaminase activity able of mitigating the effects of water deficit stress and promoting the growth of Brachiaria genotypes. Bacterial strains isolated from Brachiaria genotypes were screened in vitro for ACC deaminase activity, and the presence of the acdS gene was confirmed via polymerase chain reaction (PCR) analysis. The bacterial isolates were screened for in vitro tolerance to water deficit stress, using 10% polyethylene glycol 8000 (PEG 8000) in association with B. ruziziensis and the effects of bacterial inoculation were assessed based on plant height and fresh biomass accumulation. Additionally, the association between endophytic bacterial strains and Brachiaria genotypes was evaluated using confocal laser microscope. The results showed that among the 213 strains tested, 32 demonstrate the ability to degrade ACC into α-ketobutyrate. ACC deaminase activity was detected in 17 strains, with values ranging from 1.98 to 102.52 μmol α-ketobutyrate mg ⁻¹ protein h ⁻¹ . The presence of the acd S gene was confirmed in nine strains. The strains NRB142 ( Paraburkholderia silvatlantica ), NRB223 ( Azospirillum melinis ), and BR11790 ( Herbaspirillum frisingense GSF30 T ) exhibited the most significant promotion of plant development in B . ruziziensis under water deficit stress mediated by 10% PEG 8000. Confocal microscopy analysis revealed the rhizospheric and inner root colonization of B . ruziziensis and B . brizantha cv. Paiaguás by the NRB142 mCherry-labeled strain. This study showed no predominance of a specific group of bacterial strains in terms of ACC deaminase activity. However, a subset of strains demonstrated the ability to colonize Brachiaria plants and mitigate the negative effects of water deficit stress. This study highlights the potential of ACC deaminase-producing bacteria in alleviating water deficit stress in Brachiaria plants supporting their use as a promising strategy for improving plant resilience under drought conditions.
650 $a1-aminocyclopropane-1-carboxylic acid
650 $aPastures
650 $aPlant growth-promoting rhizobacteria
650 $aPolyethylene glycol
653 $aEthylene stress
653 $aPlant colonization
700 1 $aVIDAL, M. S.
700 1 $aBALDANI, J. I.
773 $tFrontiers Frontiers in Plant Science$gv. 16, 18 august, 2025.
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| 1. |  | LINS, P. M. P.; VIEGAS, I. de J. M.; THOMAZ, M. A. A.; FRAZÃO, D. A. C.; PAULA, P. W. R. de; FARO, M. P.; GOES, A. V. M.; OLIVEIRA, C. P. de. Efeito de doses de boro na produção de matéria seca de plantas jovens de pupunheira (Bactris gasipaes). In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 27., 1999, Brasília, DF [Anais]. Brasília, DF: Embrapa Cerrados: UNB, 1999.| Tipo: Resumo em Anais de Congresso |
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