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 | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
30/06/2025 |
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Data da última atualização: |
30/06/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LYSAK, D. H.; MICHAL, C. A.; MARABLE, K.; GRISI, M.; FARSI, R.; COSTA, P. M.; PELLIZZARI, J.; MOXLEY-PAQUETE, V.; DOWNEY, K.; RONDA, K.; MEULEN, O. V.; WOLF, W. W.; STEINER, K.; KOCK, F. V. C.; MORAES, T. B.; COLNAGO, L. A.; SCHUDERER, F.; WIRTH, T.; SIMPSON, M.; ANDERS, J. |
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Afiliação: |
UNIVERSITY OF TORONTO SCARBOROUGH; DEPARTMENT OF PHYSICS AND ASTRONOMY, UNIVERSITY OF BRITISH COLUMBIA; ANNAIDA TECHNOLOGIES; ANNAIDA TECHNOLOGIES; ANNAIDA TECHNOLOGIES; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH, TORONTO; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH; UNIVERSIDADE DE SÃO PAULO; LUIZ ALBERTO COLNAGO, CNPDIA; UNIVERSITY OF STUTTGART; UNIVERSITY OF STUTTGART; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH; UNIVERSITY OF STUTTGART. |
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Título: |
Toward “Receive-Only” Nuclear Magnetic Resonance Complementary Metal-Oxide-Semiconductor Microcoil Arrays for High-Throughput Analysis of Environmental Samples. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Analitical Chemistry, v. 97, 2025. |
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Páginas: |
11643−11652 |
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DOI: |
https://doi.org/10.1021/acs.analchem.5c00955 |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT: Daphnia magna neonates and eggs are critical life stages that show different susceptibilities to toxins and stressors compared to adults. Nuclear magnetic resonance (NMR) spectroscopy has unique potential to uncover the underlying biochemical causes, but such very small, masslimited samples are challenging to study. While the enhanced mass sensitivity of microcoils leads to markedly improved NMR analysis, experiments are often still long, leading to low throughput. Microcoil arrays can improve throughput by allowing concurrent analysis of multiple samples, but practical and economical challenges, including expensive receivers and lack of physical space, are prohibitive. Here, approaches to overcome these challenges were examined. First, “receive-only” planar complementary metal-oxide-semiconductor (CMOS) microcoils were explored wherein the microcoil was used for detection, but an external volume coil was used for excitation. This resulted in considerably improved nutation and provided a 70% boost to the signal-to-noise ratio (SNR) compared to excitation on the planar CMOS coil. Steady-state free precession experiments further improved the SNR by a factor of at least 2.5, reducing acquisition time and improving throughput. To complete a three-coil “receive-only” array, an inexpensive ($315) software-defined radio board was adapted to act as an NMR receiver with similar performance to commercial receivers. The three-coil array was used to study three D. magna eggs concurrently, potentially opening the door for high-throughput analysis and toxicological studies, where the control and exposed cohorts can be studied concurrently, reducing day-to-day variability. Overall, microcoil arrays show considerable promise for the study of D. magna eggs and other mass-limited samples MenosABSTRACT: Daphnia magna neonates and eggs are critical life stages that show different susceptibilities to toxins and stressors compared to adults. Nuclear magnetic resonance (NMR) spectroscopy has unique potential to uncover the underlying biochemical causes, but such very small, masslimited samples are challenging to study. While the enhanced mass sensitivity of microcoils leads to markedly improved NMR analysis, experiments are often still long, leading to low throughput. Microcoil arrays can improve throughput by allowing concurrent analysis of multiple samples, but practical and economical challenges, including expensive receivers and lack of physical space, are prohibitive. Here, approaches to overcome these challenges were examined. First, “receive-only” planar complementary metal-oxide-semiconductor (CMOS) microcoils were explored wherein the microcoil was used for detection, but an external volume coil was used for excitation. This resulted in considerably improved nutation and provided a 70% boost to the signal-to-noise ratio (SNR) compared to excitation on the planar CMOS coil. Steady-state free precession experiments further improved the SNR by a factor of at least 2.5, reducing acquisition time and improving throughput. To complete a three-coil “receive-only” array, an inexpensive ($315) software-defined radio board was adapted to act as an NMR receiver with similar performance to commercial receivers. The three-coil array was used to study three D. magna eggs c... Mostrar Tudo |
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Palavras-Chave: |
D magna eggs. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02934naa a2200385 a 4500
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008 2025 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1021/acs.analchem.5c00955$2DOI
100 1 $aLYSAK, D. H.
245 $aToward “Receive-Only” Nuclear Magnetic Resonance Complementary Metal-Oxide-Semiconductor Microcoil Arrays for High-Throughput Analysis of Environmental Samples.$h[electronic resource]
260 $c2025
300 $a11643−11652
520 $aABSTRACT: Daphnia magna neonates and eggs are critical life stages that show different susceptibilities to toxins and stressors compared to adults. Nuclear magnetic resonance (NMR) spectroscopy has unique potential to uncover the underlying biochemical causes, but such very small, masslimited samples are challenging to study. While the enhanced mass sensitivity of microcoils leads to markedly improved NMR analysis, experiments are often still long, leading to low throughput. Microcoil arrays can improve throughput by allowing concurrent analysis of multiple samples, but practical and economical challenges, including expensive receivers and lack of physical space, are prohibitive. Here, approaches to overcome these challenges were examined. First, “receive-only” planar complementary metal-oxide-semiconductor (CMOS) microcoils were explored wherein the microcoil was used for detection, but an external volume coil was used for excitation. This resulted in considerably improved nutation and provided a 70% boost to the signal-to-noise ratio (SNR) compared to excitation on the planar CMOS coil. Steady-state free precession experiments further improved the SNR by a factor of at least 2.5, reducing acquisition time and improving throughput. To complete a three-coil “receive-only” array, an inexpensive ($315) software-defined radio board was adapted to act as an NMR receiver with similar performance to commercial receivers. The three-coil array was used to study three D. magna eggs concurrently, potentially opening the door for high-throughput analysis and toxicological studies, where the control and exposed cohorts can be studied concurrently, reducing day-to-day variability. Overall, microcoil arrays show considerable promise for the study of D. magna eggs and other mass-limited samples
653 $aD magna eggs
700 1 $aMICHAL, C. A.
700 1 $aMARABLE, K.
700 1 $aGRISI, M.
700 1 $aFARSI, R.
700 1 $aCOSTA, P. M.
700 1 $aPELLIZZARI, J.
700 1 $aMOXLEY-PAQUETE, V.
700 1 $aDOWNEY, K.
700 1 $aRONDA, K.
700 1 $aMEULEN, O. V.
700 1 $aWOLF, W. W.
700 1 $aSTEINER, K.
700 1 $aKOCK, F. V. C.
700 1 $aMORAES, T. B.
700 1 $aCOLNAGO, L. A.
700 1 $aSCHUDERER, F.
700 1 $aWIRTH, T.
700 1 $aSIMPSON, M.
700 1 $aANDERS, J.
773 $tAnalitical Chemistry$gv. 97, 2025.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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| Registros recuperados : 16 | |
| 11. |  | LASCA, C. C.; BARROS, B. C.; VALARINI, P. J.; CASTRO, J. L. de; CHIBA, S. Acao de fungicidas em tratamento de sementes de trigo (Triticum aestivum L.) no controle de Helminthosporium sativum Pammel, King e Bakke. O Biologico, Sao Paulo, v.51, n.9, p.225-231, set. 1985.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 12. | | LASCA, C. C.; BARROS, B. C.; VALARINI, P. J.; FREGONEZI, L. E.; CHIBA, S. Eficiencia de fungicidas em tratamento de sementes de trigo (Triticum aestivum L.) para controle de Helminthosporium sativum Pammel, King & Bakke. Biologico, Sao Paulo, v.50, n.6, p.125-130, jun. 1984.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 15. | | VALARINI, P. J.; LASCA, C. C.; VECHIATO, M. H.; SCHMIDT, J. R.; DION, P.; CHIBA, S. Tratamento de sementes de sorgo (Sorghum sp.) com fungicidas visando controle de Colletotrichum graminicola e outros fungos associados a sementes. Fitopatologia Brasileira, Brasília, v.13, n.3, p.238-243, out. 1988.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 16. | | PINTO, N. F. J. A.; MENTEN, J. O. M.; LASCA, C. C.; PEREIRA, O. P.; MORAES, M. H. D.; PEREIRA, E. S. Seleção de fungicidas para o tratamento de sementes de milho. In: CONGRESSO NACIONAL DE MILHO E SORGO, 19.; REUNIÃO TÉCNICA ANUAL DE MILHO, 37.; REUNIÃO TÉCNICA ANUAL DO SORGO, 21., 1992, Porto Alegre. Resumos. Porto Alegre: SAA, 1992. p. 98.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 16 | |
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