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 | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
30/06/2025 |
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Data da última atualização: |
30/06/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LYSAK, D. H.; MICHAL, C. A.; MARABLE, K.; GRISI, M.; FARSI, R.; COSTA, P. M.; PELLIZZARI, J.; MOXLEY-PAQUETE, V.; DOWNEY, K.; RONDA, K.; MEULEN, O. V.; WOLF, W. W.; STEINER, K.; KOCK, F. V. C.; MORAES, T. B.; COLNAGO, L. A.; SCHUDERER, F.; WIRTH, T.; SIMPSON, M.; ANDERS, J. |
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Afiliação: |
UNIVERSITY OF TORONTO SCARBOROUGH; DEPARTMENT OF PHYSICS AND ASTRONOMY, UNIVERSITY OF BRITISH COLUMBIA; ANNAIDA TECHNOLOGIES; ANNAIDA TECHNOLOGIES; ANNAIDA TECHNOLOGIES; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH, TORONTO; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH; UNIVERSIDADE DE SÃO PAULO; LUIZ ALBERTO COLNAGO, CNPDIA; UNIVERSITY OF STUTTGART; UNIVERSITY OF STUTTGART; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH; UNIVERSITY OF STUTTGART. |
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Título: |
Toward “Receive-Only” Nuclear Magnetic Resonance Complementary Metal-Oxide-Semiconductor Microcoil Arrays for High-Throughput Analysis of Environmental Samples. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Analitical Chemistry, v. 97, 2025. |
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Páginas: |
11643−11652 |
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DOI: |
https://doi.org/10.1021/acs.analchem.5c00955 |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT: Daphnia magna neonates and eggs are critical life stages that show different susceptibilities to toxins and stressors compared to adults. Nuclear magnetic resonance (NMR) spectroscopy has unique potential to uncover the underlying biochemical causes, but such very small, masslimited samples are challenging to study. While the enhanced mass sensitivity of microcoils leads to markedly improved NMR analysis, experiments are often still long, leading to low throughput. Microcoil arrays can improve throughput by allowing concurrent analysis of multiple samples, but practical and economical challenges, including expensive receivers and lack of physical space, are prohibitive. Here, approaches to overcome these challenges were examined. First, “receive-only” planar complementary metal-oxide-semiconductor (CMOS) microcoils were explored wherein the microcoil was used for detection, but an external volume coil was used for excitation. This resulted in considerably improved nutation and provided a 70% boost to the signal-to-noise ratio (SNR) compared to excitation on the planar CMOS coil. Steady-state free precession experiments further improved the SNR by a factor of at least 2.5, reducing acquisition time and improving throughput. To complete a three-coil “receive-only” array, an inexpensive ($315) software-defined radio board was adapted to act as an NMR receiver with similar performance to commercial receivers. The three-coil array was used to study three D. magna eggs concurrently, potentially opening the door for high-throughput analysis and toxicological studies, where the control and exposed cohorts can be studied concurrently, reducing day-to-day variability. Overall, microcoil arrays show considerable promise for the study of D. magna eggs and other mass-limited samples MenosABSTRACT: Daphnia magna neonates and eggs are critical life stages that show different susceptibilities to toxins and stressors compared to adults. Nuclear magnetic resonance (NMR) spectroscopy has unique potential to uncover the underlying biochemical causes, but such very small, masslimited samples are challenging to study. While the enhanced mass sensitivity of microcoils leads to markedly improved NMR analysis, experiments are often still long, leading to low throughput. Microcoil arrays can improve throughput by allowing concurrent analysis of multiple samples, but practical and economical challenges, including expensive receivers and lack of physical space, are prohibitive. Here, approaches to overcome these challenges were examined. First, “receive-only” planar complementary metal-oxide-semiconductor (CMOS) microcoils were explored wherein the microcoil was used for detection, but an external volume coil was used for excitation. This resulted in considerably improved nutation and provided a 70% boost to the signal-to-noise ratio (SNR) compared to excitation on the planar CMOS coil. Steady-state free precession experiments further improved the SNR by a factor of at least 2.5, reducing acquisition time and improving throughput. To complete a three-coil “receive-only” array, an inexpensive ($315) software-defined radio board was adapted to act as an NMR receiver with similar performance to commercial receivers. The three-coil array was used to study three D. magna eggs c... Mostrar Tudo |
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Palavras-Chave: |
D magna eggs. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02934naa a2200385 a 4500
001 2176909
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008 2025 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1021/acs.analchem.5c00955$2DOI
100 1 $aLYSAK, D. H.
245 $aToward “Receive-Only” Nuclear Magnetic Resonance Complementary Metal-Oxide-Semiconductor Microcoil Arrays for High-Throughput Analysis of Environmental Samples.$h[electronic resource]
260 $c2025
300 $a11643−11652
520 $aABSTRACT: Daphnia magna neonates and eggs are critical life stages that show different susceptibilities to toxins and stressors compared to adults. Nuclear magnetic resonance (NMR) spectroscopy has unique potential to uncover the underlying biochemical causes, but such very small, masslimited samples are challenging to study. While the enhanced mass sensitivity of microcoils leads to markedly improved NMR analysis, experiments are often still long, leading to low throughput. Microcoil arrays can improve throughput by allowing concurrent analysis of multiple samples, but practical and economical challenges, including expensive receivers and lack of physical space, are prohibitive. Here, approaches to overcome these challenges were examined. First, “receive-only” planar complementary metal-oxide-semiconductor (CMOS) microcoils were explored wherein the microcoil was used for detection, but an external volume coil was used for excitation. This resulted in considerably improved nutation and provided a 70% boost to the signal-to-noise ratio (SNR) compared to excitation on the planar CMOS coil. Steady-state free precession experiments further improved the SNR by a factor of at least 2.5, reducing acquisition time and improving throughput. To complete a three-coil “receive-only” array, an inexpensive ($315) software-defined radio board was adapted to act as an NMR receiver with similar performance to commercial receivers. The three-coil array was used to study three D. magna eggs concurrently, potentially opening the door for high-throughput analysis and toxicological studies, where the control and exposed cohorts can be studied concurrently, reducing day-to-day variability. Overall, microcoil arrays show considerable promise for the study of D. magna eggs and other mass-limited samples
653 $aD magna eggs
700 1 $aMICHAL, C. A.
700 1 $aMARABLE, K.
700 1 $aGRISI, M.
700 1 $aFARSI, R.
700 1 $aCOSTA, P. M.
700 1 $aPELLIZZARI, J.
700 1 $aMOXLEY-PAQUETE, V.
700 1 $aDOWNEY, K.
700 1 $aRONDA, K.
700 1 $aMEULEN, O. V.
700 1 $aWOLF, W. W.
700 1 $aSTEINER, K.
700 1 $aKOCK, F. V. C.
700 1 $aMORAES, T. B.
700 1 $aCOLNAGO, L. A.
700 1 $aSCHUDERER, F.
700 1 $aWIRTH, T.
700 1 $aSIMPSON, M.
700 1 $aANDERS, J.
773 $tAnalitical Chemistry$gv. 97, 2025.
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Embrapa Instrumentação (CNPDIA) |
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| 1. |  | SILVA, F. N. R.; OLIVEIRA SOBRINHO, S. R. de; SILVA, J. A. da; OLIVEIRA, M. B. de; CARVALHO, D. A. de; CAMPELO, J. E. G.; VIOLA, T. H.; ARAUJO, A. M. de. Análise de agrupamento aplicado à seleção dos fundadores de populações de galinhas caipiras. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 4., 2016, Curitiba. Recursos genéticos no Brasil: a base para o desenvolvimento sustentável: anais. Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2016. p. 40.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 2. | | SILVA, J. A. da; IBIAPINA NETO, V.; RIBEIRO, A. J. de S.; SILVA JÚNIOR, E. C. da; PEREIRA, E. T. da S.; COSTA, L. M. S. da; CARVALHO, F. L. de A.; SILVA, N. da R.; VIOLA, T. H.; ARAUJO NETO, R. B. de. Desempenho de galinhas caipiras alimentadas com folíolos de angico na ração. In: JORNADA CIENTÍFICA DA EMBRAPA MEIO-NORTE, 2., 2016, Teresina. Anais da II Jornada Científica da Embrapa Meio-Norte. Teresina: Embrapa Meio-Norte, 2016. p. 61-63.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| Registros recuperados : 2 | |
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