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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
11/06/2025 |
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Data da última atualização: |
11/06/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
NICOLÁS, A. C. C. V.; LEME, L. de O.; MOURA, A. O.; KUSSANO, N. R.; SIQUEIRA, E. de; FRANCO, M. M.; DODE, M. A. N. |
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Afiliação: |
ANA CAROLINE CHAVES VALL NICOLÁS, UNIVERSITY OF BRASÍLIA - UNB; LIGIANE DE OLIVEIRA LEME, UNIVERSITY OF SÃO PAULO - USP; AMANDA OLIVEIRA MOURA, FEDERAL UNIVERSITY OF UBERLÂNDIA - UFU; NAYARA RIBEIRO KUSSANO; EMIVALDO DE SIQUEIRA, EMBRIOTEC ANIMAL REPRODUCTION; MAURICIO MACHAIM FRANCO, CENARGEN; MARGOT ALVES NUNES DODE, CENARGEN. |
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Título: |
Colony-stimulating factor 2 (CSF2) does not significantly affect cellular and molecular parameters, or blastocyst rates under in vitro culture conditions with reduced nutrient concentrations. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Theriogenology, v. 243, 117464, 2025. |
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DOI: |
https://doi.org/10.1016/j.theriogenology.2025.117464 |
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Idioma: |
Inglês |
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Conteúdo: |
Colony-stimulating factor 2 (CSF2) is an embryokine used between days 5 and 7 of culture to improve embryonic development. We evaluated its use from Day 4 and throughout the culture period in a reduced nutrient concentrations culture medium. Presumptive zygotes were assigned to three treatments: Control – synthetic oviduct fluid (SOF), CSF2 D1 (SOF + CSF2 from Day 1–7), CSF2 D4 (SOF + CSF2 from Day 4–7). Cleavage rates on Day 4 and blastocyst rates on days 6 and 7 were evaluated. On Day 7, expanded blastocysts were used to assess mitochondrial activity, lipid quantification, total cell number, gene expression and DNA methylation patterns. Embryo development data were analyzed using the chi-square test (P ≤ 0.05). Data normally distributed were analyzed with ANOVA and Tukey's test, while non-normally distributed data were evaluated using the Kruskal-Wallis and Mann-Whitney tests. Cleavage and blastocyst rates on Day 7 were similar (P > 0.05) among treatments. Of 11 genes evaluated, only Placenta-specific 8 presented a lower number of transcripts (P ≤ 0.05) in embryos cultured with CSF2 from Day 4. Assessments of DNA methylation, lipid droplet staining, mitochondrial activity and total cell number showed no significant differences among treatments (P > 0.05). In conclusion, CSF2 had no effect on the assessed parameters when a culture medium with reduced nutrient concentrations was used, and can be used throughout the entire culture period without affecting embryo production and developmental kinetics compared to CSF2 added on Day 4. MenosColony-stimulating factor 2 (CSF2) is an embryokine used between days 5 and 7 of culture to improve embryonic development. We evaluated its use from Day 4 and throughout the culture period in a reduced nutrient concentrations culture medium. Presumptive zygotes were assigned to three treatments: Control – synthetic oviduct fluid (SOF), CSF2 D1 (SOF + CSF2 from Day 1–7), CSF2 D4 (SOF + CSF2 from Day 4–7). Cleavage rates on Day 4 and blastocyst rates on days 6 and 7 were evaluated. On Day 7, expanded blastocysts were used to assess mitochondrial activity, lipid quantification, total cell number, gene expression and DNA methylation patterns. Embryo development data were analyzed using the chi-square test (P ≤ 0.05). Data normally distributed were analyzed with ANOVA and Tukey's test, while non-normally distributed data were evaluated using the Kruskal-Wallis and Mann-Whitney tests. Cleavage and blastocyst rates on Day 7 were similar (P > 0.05) among treatments. Of 11 genes evaluated, only Placenta-specific 8 presented a lower number of transcripts (P ≤ 0.05) in embryos cultured with CSF2 from Day 4. Assessments of DNA methylation, lipid droplet staining, mitochondrial activity and total cell number showed no significant differences among treatments (P > 0.05). In conclusion, CSF2 had no effect on the assessed parameters when a culture medium with reduced nutrient concentrations was used, and can be used throughout the entire culture period without affecting embryo production an... Mostrar Tudo |
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Palavras-Chave: |
Bovine; Embryokines; Preimplantation embryo. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02401naa a2200241 a 4500
001 2176554
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024 7 $ahttps://doi.org/10.1016/j.theriogenology.2025.117464$2DOI
100 1 $aNICOLÁS, A. C. C. V.
245 $aColony-stimulating factor 2 (CSF2) does not significantly affect cellular and molecular parameters, or blastocyst rates under in vitro culture conditions with reduced nutrient concentrations.$h[electronic resource]
260 $c2025
520 $aColony-stimulating factor 2 (CSF2) is an embryokine used between days 5 and 7 of culture to improve embryonic development. We evaluated its use from Day 4 and throughout the culture period in a reduced nutrient concentrations culture medium. Presumptive zygotes were assigned to three treatments: Control – synthetic oviduct fluid (SOF), CSF2 D1 (SOF + CSF2 from Day 1–7), CSF2 D4 (SOF + CSF2 from Day 4–7). Cleavage rates on Day 4 and blastocyst rates on days 6 and 7 were evaluated. On Day 7, expanded blastocysts were used to assess mitochondrial activity, lipid quantification, total cell number, gene expression and DNA methylation patterns. Embryo development data were analyzed using the chi-square test (P ≤ 0.05). Data normally distributed were analyzed with ANOVA and Tukey's test, while non-normally distributed data were evaluated using the Kruskal-Wallis and Mann-Whitney tests. Cleavage and blastocyst rates on Day 7 were similar (P > 0.05) among treatments. Of 11 genes evaluated, only Placenta-specific 8 presented a lower number of transcripts (P ≤ 0.05) in embryos cultured with CSF2 from Day 4. Assessments of DNA methylation, lipid droplet staining, mitochondrial activity and total cell number showed no significant differences among treatments (P > 0.05). In conclusion, CSF2 had no effect on the assessed parameters when a culture medium with reduced nutrient concentrations was used, and can be used throughout the entire culture period without affecting embryo production and developmental kinetics compared to CSF2 added on Day 4.
653 $aBovine
653 $aEmbryokines
653 $aPreimplantation embryo
700 1 $aLEME, L. de O.
700 1 $aMOURA, A. O.
700 1 $aKUSSANO, N. R.
700 1 $aSIQUEIRA, E. de
700 1 $aFRANCO, M. M.
700 1 $aDODE, M. A. N.
773 $tTheriogenology$gv. 243, 117464, 2025.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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