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Registro Completo |
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Biblioteca(s): |
Embrapa Agroindústria Tropical. |
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Data corrente: |
16/05/2025 |
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Data da última atualização: |
16/05/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BUARQUE, F. S.; SILVA, R. L.; BRIGIDA, A. I. S.; AMARAL, P.; COELHO, M. A. Z. |
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Afiliação: |
FILIPE SMITH BUARQUE, FEDERAL UNIVERSITY OF RIO DE JANEIRO; ROSELI LOPES DA SILVA, FEDERAL UNIVERSITY OF RIO DE JANEIRO; ANA IRAIDY SANTA BRIGIDA, CNPAT; PRISCILLA AMARAL, FEDERAL UNIVERSITY OF RIO DE JANEIRO; MARIA ALICE ZARUR COELHO, FEDERAL UNIVERSITY OF RIO DE JANEIRO. |
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Título: |
The Effect of Agitation and the Use of Perfl uorodecalin on Lipase Production by Yarrowia lipolytica in a Bioreactor. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Processes, 13, 865, 2025. |
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DOI: |
https://doi.org/10.3390/pr13030865 |
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Idioma: |
Inglês |
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Conteúdo: |
Lipase production by the strictly aerobic yeast Yarrowia lipolytica is closely related to the content of dissolved oxygen in the culture medium. Some strategies to improve oxygen transfer to microorganisms have already been used, such as the use of perfl uoro- carbons (PFCs). The present work investigates the infl uence of agitation speed and the use of perfl uorodecalin (PFC) on the profi le of the produced lipases. Lipase production increased 2.5-fold with a higher agitation speed (550 to 650 rpm) without PFCs in the medium. The presence of an oxygen carrier led to a signifi cant 91% increase in lipase production at lower shaking speeds compared to the assay without PFC; however, an increase in lipase production was not detected with PFC at 650 rpm. The protein profi les exhibited typical bands for two lipases produced (near 40 and 60 kDa), and these bands became more intense when PFC was added during production, as a result of the large enhancement in lipolytic activity. Additionally, the protein profi les obtained from extracts at 650 rpm were clearer and more selective regardless of the presence of PFC, suggesting an enhancement in specifi c activity associated with increased shaking. These fi ndings high- light the signifi cant impact of oxygen availability on lipase production, offering valuable insights for industrial applications. |
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Palavras-Chave: |
Perfluorodecalin. |
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Thesagro: |
Lípase. |
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Thesaurus Nal: |
Yarrowia lipolytica. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02023naa a2200217 a 4500
001 2175687
005 2025-05-16
008 2025 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/pr13030865$2DOI
100 1 $aBUARQUE, F. S.
245 $aThe Effect of Agitation and the Use of Perfl uorodecalin on Lipase Production by Yarrowia lipolytica in a Bioreactor.$h[electronic resource]
260 $c2025
520 $aLipase production by the strictly aerobic yeast Yarrowia lipolytica is closely related to the content of dissolved oxygen in the culture medium. Some strategies to improve oxygen transfer to microorganisms have already been used, such as the use of perfl uoro- carbons (PFCs). The present work investigates the infl uence of agitation speed and the use of perfl uorodecalin (PFC) on the profi le of the produced lipases. Lipase production increased 2.5-fold with a higher agitation speed (550 to 650 rpm) without PFCs in the medium. The presence of an oxygen carrier led to a signifi cant 91% increase in lipase production at lower shaking speeds compared to the assay without PFC; however, an increase in lipase production was not detected with PFC at 650 rpm. The protein profi les exhibited typical bands for two lipases produced (near 40 and 60 kDa), and these bands became more intense when PFC was added during production, as a result of the large enhancement in lipolytic activity. Additionally, the protein profi les obtained from extracts at 650 rpm were clearer and more selective regardless of the presence of PFC, suggesting an enhancement in specifi c activity associated with increased shaking. These fi ndings high- light the signifi cant impact of oxygen availability on lipase production, offering valuable insights for industrial applications.
650 $aYarrowia lipolytica
650 $aLípase
653 $aPerfluorodecalin
700 1 $aSILVA, R. L.
700 1 $aBRIGIDA, A. I. S.
700 1 $aAMARAL, P.
700 1 $aCOELHO, M. A. Z.
773 $tProcesses, 13, 865, 2025.
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Registro original: |
Embrapa Agroindústria Tropical (CNPAT) |
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