|
Registro Completo |
|
Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Instrumentação. |
|
Data corrente: |
12/05/2025 |
|
Data da última atualização: |
12/05/2025 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
FAYER, L.; VASCONCELLOS, R.; OLIVEIRA, E. R. de; FERREIRA, C. S. A.; SOUZA, N. L. G. D. de; MANHABOSCO, T. M.; OLIVEIRA, L. F. C. de; MARTINS, M. A.; BRANDAO, H. de M.; MUNK, M. |
|
Afiliação: |
FEDERAL UNIVERSITY OF JUIZ DE FORA; FEDERAL UNIVERSITY OF JUIZ DE FORA; FEDERAL UNIVERSITY OF JUIZ DE FORA; FEDERAL UNIVERSITY OF JUIZ DE FORA; FEDERAL UNIVERSITY OF TOCANTIS; FEDERAL UNIVERSITY OF OURO PRETO; FEDERAL UNIVERSITY OF JUIZ DE FORA; MARIA ALICE MARTINS, CNPDIA; HUMBERTO DE MELLO BRANDAO, CNPGL; FEDERAL UNIVERSITY OF JUIZ DE FORA. |
|
Título: |
Cotton cellulose nanofiber/chitosan scaffolds for skin tissueengineering and wound healing applications. |
|
Ano de publicação: |
2025 |
|
Fonte/Imprenta: |
BiomedIcal Materials, v. 20, 015024, 2025. |
|
Páginas: |
18 p. |
|
DOI: |
https://doi.org/10.1088/1748-605X/ad9da4 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Chitosan (CS) is a promising polymeric biomaterial for use in scaffolds for in vitro skin models and wound dressings, owing to its non-antigenic and antimicrobial properties. However, CS often exhibits insufficient physicochemical properties, mechanical strength, and bioactivity, limiting its efficacy in demanding applications. To address these challenges, cotton cellulose nanofibers (CNFs) represent a promising nanomaterial for enhancing CS-based scaffolds in tissue engineering. CNF offers superior stiffness, and mechanical properties that enhance cellular adhesion and proliferation, both crucial for effective tissue regeneration and healing. This study aimed to develop and characterize a scaffold combining cotton CNF and CS, focusing on its cytocompatibility with human fibroblasts and keratinocytes. The cotton CNF/CS scaffold was fabricated using the casting technique, and its physicochemical properties and cellular compatibility were assessed in vitro. The results demonstrated that incorporating cotton CNF significantly enhanced the stability of the CS matrix. The CS scaffold with 1000 µg ml−1 of cotton CNF exhibited increased roughness and reduced rupture strain compared to the pure CS scaffold. The cotton CNF/CS scaffold effectively promoted the adhesion, viability, proliferation, migration, and collagen synthesis of skin cells. Notably, increased cell viability was observed in human fibroblasts cultured on scaffolds with higher concentrations of cotton CNF (100 and 1000 µg ml−1 ). Based on the findings, the cotton CNF/CS scaffold demonstrates enhanced physicochemical properties and bioactivity, making it a promising candidate for the development of in vitro human skin models and wound healing dressings. MenosChitosan (CS) is a promising polymeric biomaterial for use in scaffolds for in vitro skin models and wound dressings, owing to its non-antigenic and antimicrobial properties. However, CS often exhibits insufficient physicochemical properties, mechanical strength, and bioactivity, limiting its efficacy in demanding applications. To address these challenges, cotton cellulose nanofibers (CNFs) represent a promising nanomaterial for enhancing CS-based scaffolds in tissue engineering. CNF offers superior stiffness, and mechanical properties that enhance cellular adhesion and proliferation, both crucial for effective tissue regeneration and healing. This study aimed to develop and characterize a scaffold combining cotton CNF and CS, focusing on its cytocompatibility with human fibroblasts and keratinocytes. The cotton CNF/CS scaffold was fabricated using the casting technique, and its physicochemical properties and cellular compatibility were assessed in vitro. The results demonstrated that incorporating cotton CNF significantly enhanced the stability of the CS matrix. The CS scaffold with 1000 µg ml−1 of cotton CNF exhibited increased roughness and reduced rupture strain compared to the pure CS scaffold. The cotton CNF/CS scaffold effectively promoted the adhesion, viability, proliferation, migration, and collagen synthesis of skin cells. Notably, increased cell viability was observed in human fibroblasts cultured on scaffolds with higher concentrations of cotton CNF (100 and 100... Mostrar Tudo |
|
Palavras-Chave: |
Biomedical nanomaterials; Biomimetic. |
|
Categoria do assunto: |
--
F Plantas e Produtos de Origem Vegetal |
|
Marc: |
LEADER 02588naa a2200277 a 4500
001 2175500
005 2025-05-12
008 2025 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1088/1748-605X/ad9da4$2DOI
100 1 $aFAYER, L.
245 $aCotton cellulose nanofiber/chitosan scaffolds for skin tissueengineering and wound healing applications.$h[electronic resource]
260 $c2025
300 $a18 p.
520 $aChitosan (CS) is a promising polymeric biomaterial for use in scaffolds for in vitro skin models and wound dressings, owing to its non-antigenic and antimicrobial properties. However, CS often exhibits insufficient physicochemical properties, mechanical strength, and bioactivity, limiting its efficacy in demanding applications. To address these challenges, cotton cellulose nanofibers (CNFs) represent a promising nanomaterial for enhancing CS-based scaffolds in tissue engineering. CNF offers superior stiffness, and mechanical properties that enhance cellular adhesion and proliferation, both crucial for effective tissue regeneration and healing. This study aimed to develop and characterize a scaffold combining cotton CNF and CS, focusing on its cytocompatibility with human fibroblasts and keratinocytes. The cotton CNF/CS scaffold was fabricated using the casting technique, and its physicochemical properties and cellular compatibility were assessed in vitro. The results demonstrated that incorporating cotton CNF significantly enhanced the stability of the CS matrix. The CS scaffold with 1000 µg ml−1 of cotton CNF exhibited increased roughness and reduced rupture strain compared to the pure CS scaffold. The cotton CNF/CS scaffold effectively promoted the adhesion, viability, proliferation, migration, and collagen synthesis of skin cells. Notably, increased cell viability was observed in human fibroblasts cultured on scaffolds with higher concentrations of cotton CNF (100 and 1000 µg ml−1 ). Based on the findings, the cotton CNF/CS scaffold demonstrates enhanced physicochemical properties and bioactivity, making it a promising candidate for the development of in vitro human skin models and wound healing dressings.
653 $aBiomedical nanomaterials
653 $aBiomimetic
700 1 $aVASCONCELLOS, R.
700 1 $aOLIVEIRA, E. R. de
700 1 $aFERREIRA, C. S. A.
700 1 $aSOUZA, N. L. G. D. de
700 1 $aMANHABOSCO, T. M.
700 1 $aOLIVEIRA, L. F. C. de
700 1 $aMARTINS, M. A.
700 1 $aBRANDAO, H. de M.
700 1 $aMUNK, M.
773 $tBiomedIcal Materials$gv. 20, 015024, 2025.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Instrumentação (CNPDIA) |
|
