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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
24/03/2025 |
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Data da última atualização: |
31/03/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MOURA, A. O.; SILVA, T. C. F.; CAETANO, A. R.; KUSSANO, N. R.; DODE, M. A. N.; FRANCO, M. M. |
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Afiliação: |
AMANDA OLIVEIRA MOURA, FEDERAL UNIVERSITY OF UBERLÂNDIA; THAINARA CHRISTIE FERREIRA SILVA; ALEXANDRE RODRIGUES CAETANO, CENARGEN; NAYARA RIBEIRO KUSSANO; MARGOT ALVES NUNES DODE, CENARGEN; MAURICIO MACHAIM FRANCO, CENARGEN. |
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Título: |
Characterization of DNA methylation profile of the entire CpG island spanning the 5′ untranslated region to intron 1 of the Oct4/POU5F1 gene in bovine gametes, embryos, and somatic cells. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Animal Genetics, v. 56, e70002, 2025. |
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DOI: |
https://doi.org/10.1111/age.70002 |
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Idioma: |
Inglês |
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Conteúdo: |
Stem cells are undifferentiated cells that exhibit a bivalent chromatin state that determines their fate. These cells have potential applications in human and animal health and livestock production. Somatic cell nuclear transfer or cloning is currently being used to produce genetically edited animals. A highly differentiated genome is the main obstacle to correcting epigenetic reprogramming by enucleated oocytes during cloning. Activation of pluripotency genes in the somatic genome is a promising strategy to contribute to more efficient epigenetic reprogramming, improving this technique. Recently, epigenome editing has emerged as a new generation of clustered regularly interspaced short palindromic repeats–clustered regularly interspaced short palindromic repeats-associated protein 9 technology with the aim of modifying the cellular epigenome to turn genes on or off without modifying DNA. Here, we characterize the DNA methylation profile of the CpG island spanning the 5′ untranslated region to intron 1 of the bovine octamer-binding transcription factor (Oct4) gene in gametes, embryos, and fibroblasts. DNA methylation patterns were categorized into three levels: low (0%–20%), moderate (21%–50%), and high (51%–100%). Sperm and embryos showed a hypomethylation pattern, whereas oocytes exhibited a hypo- to moderate methylation pattern. Fetal and adult skin fibroblasts were hypomethylated and moderately methylated, respectively. These results are essential for future studies aimed at manipulating the expression of Oct4. Thus, epigenome editing can be used to turn on the Oct4 in somatic cells to generate induced pluripotent stem cells. This strategy could potentially convert a fully differentiated cell into a cell with certain degree of pluripotency, facilitating nuclear reprogramming by the enucleated oocyte and improving cloning success rates. MenosStem cells are undifferentiated cells that exhibit a bivalent chromatin state that determines their fate. These cells have potential applications in human and animal health and livestock production. Somatic cell nuclear transfer or cloning is currently being used to produce genetically edited animals. A highly differentiated genome is the main obstacle to correcting epigenetic reprogramming by enucleated oocytes during cloning. Activation of pluripotency genes in the somatic genome is a promising strategy to contribute to more efficient epigenetic reprogramming, improving this technique. Recently, epigenome editing has emerged as a new generation of clustered regularly interspaced short palindromic repeats–clustered regularly interspaced short palindromic repeats-associated protein 9 technology with the aim of modifying the cellular epigenome to turn genes on or off without modifying DNA. Here, we characterize the DNA methylation profile of the CpG island spanning the 5′ untranslated region to intron 1 of the bovine octamer-binding transcription factor (Oct4) gene in gametes, embryos, and fibroblasts. DNA methylation patterns were categorized into three levels: low (0%–20%), moderate (21%–50%), and high (51%–100%). Sperm and embryos showed a hypomethylation pattern, whereas oocytes exhibited a hypo- to moderate methylation pattern. Fetal and adult skin fibroblasts were hypomethylated and moderately methylated, respectively. These results are essential for future studies aime... Mostrar Tudo |
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Palavras-Chave: |
Bovine; Oct4. |
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Thesaurus Nal: |
DNA methylation; Epigenetics. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02667naa a2200241 a 4500
001 2174127
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024 7 $ahttps://doi.org/10.1111/age.70002$2DOI
100 1 $aMOURA, A. O.
245 $aCharacterization of DNA methylation profile of the entire CpG island spanning the 5′ untranslated region to intron 1 of the Oct4/POU5F1 gene in bovine gametes, embryos, and somatic cells.$h[electronic resource]
260 $c2025
520 $aStem cells are undifferentiated cells that exhibit a bivalent chromatin state that determines their fate. These cells have potential applications in human and animal health and livestock production. Somatic cell nuclear transfer or cloning is currently being used to produce genetically edited animals. A highly differentiated genome is the main obstacle to correcting epigenetic reprogramming by enucleated oocytes during cloning. Activation of pluripotency genes in the somatic genome is a promising strategy to contribute to more efficient epigenetic reprogramming, improving this technique. Recently, epigenome editing has emerged as a new generation of clustered regularly interspaced short palindromic repeats–clustered regularly interspaced short palindromic repeats-associated protein 9 technology with the aim of modifying the cellular epigenome to turn genes on or off without modifying DNA. Here, we characterize the DNA methylation profile of the CpG island spanning the 5′ untranslated region to intron 1 of the bovine octamer-binding transcription factor (Oct4) gene in gametes, embryos, and fibroblasts. DNA methylation patterns were categorized into three levels: low (0%–20%), moderate (21%–50%), and high (51%–100%). Sperm and embryos showed a hypomethylation pattern, whereas oocytes exhibited a hypo- to moderate methylation pattern. Fetal and adult skin fibroblasts were hypomethylated and moderately methylated, respectively. These results are essential for future studies aimed at manipulating the expression of Oct4. Thus, epigenome editing can be used to turn on the Oct4 in somatic cells to generate induced pluripotent stem cells. This strategy could potentially convert a fully differentiated cell into a cell with certain degree of pluripotency, facilitating nuclear reprogramming by the enucleated oocyte and improving cloning success rates.
650 $aDNA methylation
650 $aEpigenetics
653 $aBovine
653 $aOct4
700 1 $aSILVA, T. C. F.
700 1 $aCAETANO, A. R.
700 1 $aKUSSANO, N. R.
700 1 $aDODE, M. A. N.
700 1 $aFRANCO, M. M.
773 $tAnimal Genetics$gv. 56, e70002, 2025.
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