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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
24/03/2025 |
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Data da última atualização: |
31/03/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MOURA, A. O.; SILVA, T. C. F.; CAETANO, A. R.; KUSSANO, N. R.; DODE, M. A. N.; FRANCO, M. M. |
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Afiliação: |
AMANDA OLIVEIRA MOURA, FEDERAL UNIVERSITY OF UBERLÂNDIA; THAINARA CHRISTIE FERREIRA SILVA; ALEXANDRE RODRIGUES CAETANO, CENARGEN; NAYARA RIBEIRO KUSSANO; MARGOT ALVES NUNES DODE, CENARGEN; MAURICIO MACHAIM FRANCO, CENARGEN. |
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Título: |
Characterization of DNA methylation profile of the entire CpG island spanning the 5′ untranslated region to intron 1 of the Oct4/POU5F1 gene in bovine gametes, embryos, and somatic cells. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Animal Genetics, v. 56, e70002, 2025. |
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DOI: |
https://doi.org/10.1111/age.70002 |
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Idioma: |
Inglês |
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Conteúdo: |
Stem cells are undifferentiated cells that exhibit a bivalent chromatin state that determines their fate. These cells have potential applications in human and animal health and livestock production. Somatic cell nuclear transfer or cloning is currently being used to produce genetically edited animals. A highly differentiated genome is the main obstacle to correcting epigenetic reprogramming by enucleated oocytes during cloning. Activation of pluripotency genes in the somatic genome is a promising strategy to contribute to more efficient epigenetic reprogramming, improving this technique. Recently, epigenome editing has emerged as a new generation of clustered regularly interspaced short palindromic repeats–clustered regularly interspaced short palindromic repeats-associated protein 9 technology with the aim of modifying the cellular epigenome to turn genes on or off without modifying DNA. Here, we characterize the DNA methylation profile of the CpG island spanning the 5′ untranslated region to intron 1 of the bovine octamer-binding transcription factor (Oct4) gene in gametes, embryos, and fibroblasts. DNA methylation patterns were categorized into three levels: low (0%–20%), moderate (21%–50%), and high (51%–100%). Sperm and embryos showed a hypomethylation pattern, whereas oocytes exhibited a hypo- to moderate methylation pattern. Fetal and adult skin fibroblasts were hypomethylated and moderately methylated, respectively. These results are essential for future studies aimed at manipulating the expression of Oct4. Thus, epigenome editing can be used to turn on the Oct4 in somatic cells to generate induced pluripotent stem cells. This strategy could potentially convert a fully differentiated cell into a cell with certain degree of pluripotency, facilitating nuclear reprogramming by the enucleated oocyte and improving cloning success rates. MenosStem cells are undifferentiated cells that exhibit a bivalent chromatin state that determines their fate. These cells have potential applications in human and animal health and livestock production. Somatic cell nuclear transfer or cloning is currently being used to produce genetically edited animals. A highly differentiated genome is the main obstacle to correcting epigenetic reprogramming by enucleated oocytes during cloning. Activation of pluripotency genes in the somatic genome is a promising strategy to contribute to more efficient epigenetic reprogramming, improving this technique. Recently, epigenome editing has emerged as a new generation of clustered regularly interspaced short palindromic repeats–clustered regularly interspaced short palindromic repeats-associated protein 9 technology with the aim of modifying the cellular epigenome to turn genes on or off without modifying DNA. Here, we characterize the DNA methylation profile of the CpG island spanning the 5′ untranslated region to intron 1 of the bovine octamer-binding transcription factor (Oct4) gene in gametes, embryos, and fibroblasts. DNA methylation patterns were categorized into three levels: low (0%–20%), moderate (21%–50%), and high (51%–100%). Sperm and embryos showed a hypomethylation pattern, whereas oocytes exhibited a hypo- to moderate methylation pattern. Fetal and adult skin fibroblasts were hypomethylated and moderately methylated, respectively. These results are essential for future studies aime... Mostrar Tudo |
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Palavras-Chave: |
Bovine; Oct4. |
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Thesaurus Nal: |
DNA methylation; Epigenetics. |
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Categoria do assunto: |
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Marc: |
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| 1. |  | LEITE, A. N.; NASCIMENTO, L. da L.; SILVA, V. R. C. da; CRUZ, A. J. F.; LEAO, P. C. de S. Caracterização morfoagronômica de genótipos de uvas de mesa do Banco Ativo de Germoplasma de videira da Embrapa Semiárido. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 17., 2023, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2023. p. 42. (Embrapa Semiárido. Eventos Técnicos & Científicos, 1).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 2. | | OLIVEIRA, C. R. S. de; NASCIMENTO, L. da L.; CUNHA, M. A. C. da; MENDONÇA JÚNIOR, A. F. de; LEAO, P. C. de S. Influência de porta-enxertos no vigor, produtividade e qualidade de uvas ‘BRS Melodia’ no Vale do São Francisco. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 18., 2024, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2024. p. 11-12. (Embrapa Semiárido. Eventos Técnicos & Científicos, 3)| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 3. | | OLIVEIRA, C. R. S. de; SILVA, F. B. da; SILVA, V. R. C. da; CUNHA, M. A. C. da; NASCIMENTO, L. da L.; MENDONÇA JÚNIOR, A. F. de; LEAO, P. C. de S. Vitivinicultura: aspectos gerais e importância econômica. In: REDIN, E. (Org.). Ciências rurais no século XXI. Belo Horizonte: Poisson, 2024. v.3 Cap. 1, p. 6-13.| Tipo: Capítulo em Livro Técnico-Científico |
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| Registros recuperados : 3 | |
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