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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria Tropical. Para informações adicionais entre em contato com cnpat.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroindústria Tropical; Embrapa Gado de Leite. |
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Data corrente: |
20/03/2025 |
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Data da última atualização: |
26/03/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, J. F. M.; ALONSO, B. V.; ALMEIDA, P. A. A.; BARBOSA, I. V.; PAULA, O. A. B. de; BARBOSA, L. R.; BRUNO, L. M.; MENEZES, L. D. M.; SILVA, M. R.; COSTA, G. M. da; RODARTE, M. P.; RIBEIRO, J. B. |
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Afiliação: |
JOICE FATIMA MOREIRA SILVA, UNIVERSIDADE FEDERAL DE LAVRAS; BRUNA VIEIRA ALONSO, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; PAULA APARECIDA AZEVEDO ALMEIDA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; ISABELA VIEIRA BARBOSA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; OTAVIO AUGUSTO BRAGA DE PAULA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; LETÍCIA RIBEIRO BARBOSA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; LAURA MARIA BRUNO, CNPAT; LILIANE DENIZE MIRANDA MENEZES, INSTITUTO AGROPECUÁRIO DE MINAS GERAIS; MARCIO ROBERTO SILVA, CNPGL; GERALDO MARCIO DA COSTA, UNIVERSIDADE FEDERAL DE LAVRAS; MIRIAN PEREIRA RODARTE, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; JOAO BATISTA RIBEIRO, CNPGL. |
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Título: |
Searching for antibiotic-susceptible bioprotective lactic acid bacteria to control dangerous biological agents in artisanal cheese. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Food Microbiology, v. 130, 104762, 2025. |
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DOI: |
https://doi.org/10.1016/j.fm.2025.104762 |
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Idioma: |
Inglês |
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Conteúdo: |
Minas artisanal cheese (MAC) samples (n = 59) from 16 municipalities across five traditional MAC-producing regions in Brazil were used to prospect antibiotic-susceptible protective lactic acid bacteria (LAB) against three pathogenic bacteria found in the MAC. From 291 LAB isolates, 84 genetically diverse strains were selected via rep-PCR. MALDI-TOF identification revealed multiple species, predominantly Enterococcus faecalis (n = 37), Enterococcus faecium (n = 21), Lactiplantibacillus plantarum (n = 5) and Lacticaseibacillus rhamnosus (n = 3). The antagonistic activity of these strains was evaluated against Enterococcus faecalis ATCC 29212, Listeria monocytogenes ATCC 5779, and Escherichia coli O157:H7 using spot-on-lawn assays. Several strains showed strong inhibitory effects against E. coli and L. monocytogenes, with halo/colony ratios reaching 4.86 and 4.47, respectively. No antimicrobial peptide producing strain was observed. Antibiotic susceptibility was tested against nine antibiotics, and five strains were susceptible to all antibiotics, while 53 strains were susceptible to 5–8 antibiotics. However, five strains were resistant to all antibiotics, showing the highest resistance to gentamicin (66.7%), cotrimoxazole (58.3%), and streptomycin (57.2%). Resistance genes (aacA-aphD, ermA/B, tetM/O/K/L/ S, blaZ, and vanA/B) were screened, and 40 strains harbored at least one gene. Taken together, these results revealed three antibiotic-susceptible bioprotective lactobacilli (L. rhamnosus 52, L. plantarum 177, and L. plantarum 272G) as superior strains, whose efficacy in eliminating E. coli O157 and Listeria monocytogenes in the milk matrix between 7- and 21-days post-inoculation was confirmed. These findings confirm the potential of these autochthonous lactobacilli to improve the safety of dairy, paving the way for their applications in product development in future projects. MenosMinas artisanal cheese (MAC) samples (n = 59) from 16 municipalities across five traditional MAC-producing regions in Brazil were used to prospect antibiotic-susceptible protective lactic acid bacteria (LAB) against three pathogenic bacteria found in the MAC. From 291 LAB isolates, 84 genetically diverse strains were selected via rep-PCR. MALDI-TOF identification revealed multiple species, predominantly Enterococcus faecalis (n = 37), Enterococcus faecium (n = 21), Lactiplantibacillus plantarum (n = 5) and Lacticaseibacillus rhamnosus (n = 3). The antagonistic activity of these strains was evaluated against Enterococcus faecalis ATCC 29212, Listeria monocytogenes ATCC 5779, and Escherichia coli O157:H7 using spot-on-lawn assays. Several strains showed strong inhibitory effects against E. coli and L. monocytogenes, with halo/colony ratios reaching 4.86 and 4.47, respectively. No antimicrobial peptide producing strain was observed. Antibiotic susceptibility was tested against nine antibiotics, and five strains were susceptible to all antibiotics, while 53 strains were susceptible to 5–8 antibiotics. However, five strains were resistant to all antibiotics, showing the highest resistance to gentamicin (66.7%), cotrimoxazole (58.3%), and streptomycin (57.2%). Resistance genes (aacA-aphD, ermA/B, tetM/O/K/L/ S, blaZ, and vanA/B) were screened, and 40 strains harbored at least one gene. Taken together, these results revealed three antibiotic-susceptible bioprotective lactobacilli (... Mostrar Tudo |
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Palavras-Chave: |
Atividade antimicrobiana; Enterococci; Lactobacilo. |
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Thesagro: |
Antibiótico; Bactéria; Listeria Monocytogenes; Queijo; Resistência. |
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Thesaurus Nal: |
Escherichia coli O157. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03019naa a2200373 a 4500
001 2174091
005 2025-03-26
008 2025 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.fm.2025.104762$2DOI
100 1 $aSILVA, J. F. M.
245 $aSearching for antibiotic-susceptible bioprotective lactic acid bacteria to control dangerous biological agents in artisanal cheese.$h[electronic resource]
260 $c2025
520 $aMinas artisanal cheese (MAC) samples (n = 59) from 16 municipalities across five traditional MAC-producing regions in Brazil were used to prospect antibiotic-susceptible protective lactic acid bacteria (LAB) against three pathogenic bacteria found in the MAC. From 291 LAB isolates, 84 genetically diverse strains were selected via rep-PCR. MALDI-TOF identification revealed multiple species, predominantly Enterococcus faecalis (n = 37), Enterococcus faecium (n = 21), Lactiplantibacillus plantarum (n = 5) and Lacticaseibacillus rhamnosus (n = 3). The antagonistic activity of these strains was evaluated against Enterococcus faecalis ATCC 29212, Listeria monocytogenes ATCC 5779, and Escherichia coli O157:H7 using spot-on-lawn assays. Several strains showed strong inhibitory effects against E. coli and L. monocytogenes, with halo/colony ratios reaching 4.86 and 4.47, respectively. No antimicrobial peptide producing strain was observed. Antibiotic susceptibility was tested against nine antibiotics, and five strains were susceptible to all antibiotics, while 53 strains were susceptible to 5–8 antibiotics. However, five strains were resistant to all antibiotics, showing the highest resistance to gentamicin (66.7%), cotrimoxazole (58.3%), and streptomycin (57.2%). Resistance genes (aacA-aphD, ermA/B, tetM/O/K/L/ S, blaZ, and vanA/B) were screened, and 40 strains harbored at least one gene. Taken together, these results revealed three antibiotic-susceptible bioprotective lactobacilli (L. rhamnosus 52, L. plantarum 177, and L. plantarum 272G) as superior strains, whose efficacy in eliminating E. coli O157 and Listeria monocytogenes in the milk matrix between 7- and 21-days post-inoculation was confirmed. These findings confirm the potential of these autochthonous lactobacilli to improve the safety of dairy, paving the way for their applications in product development in future projects.
650 $aEscherichia coli O157
650 $aAntibiótico
650 $aBactéria
650 $aListeria Monocytogenes
650 $aQueijo
650 $aResistência
653 $aAtividade antimicrobiana
653 $aEnterococci
653 $aLactobacilo
700 1 $aALONSO, B. V.
700 1 $aALMEIDA, P. A. A.
700 1 $aBARBOSA, I. V.
700 1 $aPAULA, O. A. B. de
700 1 $aBARBOSA, L. R.
700 1 $aBRUNO, L. M.
700 1 $aMENEZES, L. D. M.
700 1 $aSILVA, M. R.
700 1 $aCOSTA, G. M. da
700 1 $aRODARTE, M. P.
700 1 $aRIBEIRO, J. B.
773 $tFood Microbiology$gv. 130, 104762, 2025.
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