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Registro Completo |
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
14/02/2025 |
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Data da última atualização: |
14/02/2025 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
MORAIS, I. J.; FERREIRA, Y. F. M.; NAKASU, E. Y. T.; NAGATA, T.; INOUE-NAGATA, A. K. |
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Afiliação: |
IVAIR J. MORAIS, UNIVERSIDADE DE BRASÍLIA; YANCA F. M. FERREIRA; ERICH YUKIO TEMPEL NAKASU, CNPH; TATSUYA NAGATA, UNIVERSIDADE DE BRASÍLIA; ALICE KAZUKO INOUE NAGATA, CNPH. |
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Título: |
Agroinoculation of geminiviral infectious clones into plants. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
In: FONTES, E. P. B.; MÄKINEN, K.(ed.). Plant-virus interactions. London: Humana Press, 2024. |
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Páginas: |
p. 65-70 |
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Série: |
(Methods in molecular biology, 2724). |
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ISBN: |
978-1-0716-3485-1 |
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DOI: |
10.1007/978-1-0716-3485-1_5 |
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Idioma: |
Inglês |
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Conteúdo: |
Most geminiviruses are not transmitted by mechanical inoculation. Therefore, pathogenicity and plant–pathogen interaction studies rely on agroinoculation using infectious clones, which involves cloning the geminiviral genome in a binary vector (see previous chapter for details). A suspension containing the infectious clone inserted into Agrobacterium tumefaciens cells is then inoculated into plants, i.e., agroinoculated. Below is a simple protocol for agroinoculation of an infectious geminivirus clone into plants. |
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Palavras-Chave: |
Geminivirus; Interação planta patógeno. |
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Thesagro: |
Agrobacterium Tumefaciens; Patogenicidade; Patógeno; Planta; Vírus. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
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490 $a(Methods in molecular biology, 2724).
520 $aMost geminiviruses are not transmitted by mechanical inoculation. Therefore, pathogenicity and plant–pathogen interaction studies rely on agroinoculation using infectious clones, which involves cloning the geminiviral genome in a binary vector (see previous chapter for details). A suspension containing the infectious clone inserted into Agrobacterium tumefaciens cells is then inoculated into plants, i.e., agroinoculated. Below is a simple protocol for agroinoculation of an infectious geminivirus clone into plants.
650 $aAgrobacterium Tumefaciens
650 $aPatogenicidade
650 $aPatógeno
650 $aPlanta
650 $aVírus
653 $aGeminivirus
653 $aInteração planta patógeno
700 1 $aFERREIRA, Y. F. M.
700 1 $aNAKASU, E. Y. T.
700 1 $aNAGATA, T.
700 1 $aINOUE-NAGATA, A. K.
773 $tIn: FONTES, E. P. B.; MÄKINEN, K.(ed.). Plant-virus interactions. London: Humana Press, 2024.
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Registro original: |
Embrapa Hortaliças (CNPH) |
