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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
29/01/2025 |
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Data da última atualização: |
10/02/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FERRARI, V. B.; LIMA, L. M. dos S.; MARQUES, K. de M.; GUTIERRES, F. C.; GUERINI, G. G.; SILVEIRA, M. A. V.; FIGUEIREDO, G. M. de; VITAL, V. G.; ROSWELL, M. R.; MELO, I. S. de; OKAMOTO, D. N.; VASCONCELLOS, S. P. de. |
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Afiliação: |
VITOR BAPTISTA FERRARI, UNIVERSIDADE FEDERAL DE SÃO PAULO; LIDIANE MARIA DOS SANTOS LIMA, UNIVERSIDADE FEDERAL DE SÃO PAULO; KELLY DE MATOS MARQUES, UNIVERSIDADE FEDERAL DE SÃO PAULO; FERNANDA CAMILA GUTIERRES, UNIVERSIDADE FEDERAL DE SÃO PAULO; GUILHERME GONÇALVES GUERINI, UNIVERSIDADE FEDERAL DE SÃO PAULO; MARGHUEL APARECIDA VIEIRA SILVEIRA, UNIVERSIDADE FEDERAL DE SÃO PAULO; GUILHERME MASCALCHI DE FIGUEIREDO, UNIVERSIDADE FEDERAL DE SÃO PAULO; VITOR GONÇALVES VITAL, UNIVERSIDADE FEDERAL DE SÃO PAULO; MARIANA ROCHA ROSWELL, UNIVERSIDADE FEDERAL DE SÃO PAULO; ITAMAR SOARES DE MELO, CNPMA; DEBORA NOMA OKAMOTO, UNIVERSIDADE FEDERAL DE SÃO PAULO; SUZAN PANTAROTO DE VASCONCELLOS, UNIVERSIDADE FEDERAL DE SÃO PAULO. |
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Título: |
Caatinga, Amazon and Atlantic Forest as natural sources for microbial lignocellulolytic enzymes. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Archives of Microbiology, v. 206, n. 4, article 161, 2024. |
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ISSN: |
0302-8933 |
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DOI: |
https://doi.org/10.1007/s00203-024-03883-y |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Brazilian biomes are important sources for environmental microorganisms, including efficient metabolic machineries, like actinomycetes. These bacteria are known for their abilities to produce many bioactive compounds, including enzymes with multiple industrial applications. The present work aimed to evaluate lignocellulolytic abilities of actinomycetes isolated from soil and rhizosphere samples collected at Caatinga, Atlantic and Amazon Forest. Laccase (Lac), lignin peroxidase (LiP), manganese peroxidase (MnP) and cellulase were evaluated for their efficiency. These enzymes have an essential role in lignin decomposition, through oxidation of phenolic and non-phenolic compounds, as well as enzymatic hydrolysis of vegetal biomass. In this sense, a total of 173 actinomycetes were investigated. Eleven (11) of them were selected by their enzymatic performance. The actinomycete AC166 displayed some activity in all analysed scenarios in terms of Lac, MnP and LiP activity, while AC171 was selected as the most promising strain, showing the following activities: 29.7 U.L-1 for Lac; 2.5 U.L-1 for LiP and 23 U.L-1 for MnP. Cellulolytic activities were evaluated at two pH conditions, 4.8 and 7.4, obtaining the following results: 25 U.L-1 and 71 U.L-1, respectively. Thermostability (4, 30 and 60 o C) and salinity concentrations (0 to 4 M) and pH variation (2.0 to 9.0) stabilities of the obtained LiP and Lac enzymatic extracts were also verified. The actinomycete strain AC171 displayed an adaptable response in distinct pH and salt profiles, indicating that bacterial LiP was some halophilic type. Additionally, the strain AC149 produced an alkali and extreme halophilic lignin peroxidase, which are promising profiles for their future application under lignocellulosic biomass at bioethanol biorefineries. MenosAbstract: Brazilian biomes are important sources for environmental microorganisms, including efficient metabolic machineries, like actinomycetes. These bacteria are known for their abilities to produce many bioactive compounds, including enzymes with multiple industrial applications. The present work aimed to evaluate lignocellulolytic abilities of actinomycetes isolated from soil and rhizosphere samples collected at Caatinga, Atlantic and Amazon Forest. Laccase (Lac), lignin peroxidase (LiP), manganese peroxidase (MnP) and cellulase were evaluated for their efficiency. These enzymes have an essential role in lignin decomposition, through oxidation of phenolic and non-phenolic compounds, as well as enzymatic hydrolysis of vegetal biomass. In this sense, a total of 173 actinomycetes were investigated. Eleven (11) of them were selected by their enzymatic performance. The actinomycete AC166 displayed some activity in all analysed scenarios in terms of Lac, MnP and LiP activity, while AC171 was selected as the most promising strain, showing the following activities: 29.7 U.L-1 for Lac; 2.5 U.L-1 for LiP and 23 U.L-1 for MnP. Cellulolytic activities were evaluated at two pH conditions, 4.8 and 7.4, obtaining the following results: 25 U.L-1 and 71 U.L-1, respectively. Thermostability (4, 30 and 60 o C) and salinity concentrations (0 to 4 M) and pH variation (2.0 to 9.0) stabilities of the obtained LiP and Lac enzymatic extracts were also verified. The actinomycete strain AC171 dis... Mostrar Tudo |
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Thesagro: |
Actinomiceto; Celulase; Peroxidase. |
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Thesaurus Nal: |
Actinomyces; Cellulases; Laccase; Lignin peroxidase; Manganese peroxidase. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
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001 2172061
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022 $a0302-8933
024 7 $ahttps://doi.org/10.1007/s00203-024-03883-y$2DOI
100 1 $aFERRARI, V. B.
245 $aCaatinga, Amazon and Atlantic Forest as natural sources for microbial lignocellulolytic enzymes.$h[electronic resource]
260 $c2024
520 $aAbstract: Brazilian biomes are important sources for environmental microorganisms, including efficient metabolic machineries, like actinomycetes. These bacteria are known for their abilities to produce many bioactive compounds, including enzymes with multiple industrial applications. The present work aimed to evaluate lignocellulolytic abilities of actinomycetes isolated from soil and rhizosphere samples collected at Caatinga, Atlantic and Amazon Forest. Laccase (Lac), lignin peroxidase (LiP), manganese peroxidase (MnP) and cellulase were evaluated for their efficiency. These enzymes have an essential role in lignin decomposition, through oxidation of phenolic and non-phenolic compounds, as well as enzymatic hydrolysis of vegetal biomass. In this sense, a total of 173 actinomycetes were investigated. Eleven (11) of them were selected by their enzymatic performance. The actinomycete AC166 displayed some activity in all analysed scenarios in terms of Lac, MnP and LiP activity, while AC171 was selected as the most promising strain, showing the following activities: 29.7 U.L-1 for Lac; 2.5 U.L-1 for LiP and 23 U.L-1 for MnP. Cellulolytic activities were evaluated at two pH conditions, 4.8 and 7.4, obtaining the following results: 25 U.L-1 and 71 U.L-1, respectively. Thermostability (4, 30 and 60 o C) and salinity concentrations (0 to 4 M) and pH variation (2.0 to 9.0) stabilities of the obtained LiP and Lac enzymatic extracts were also verified. The actinomycete strain AC171 displayed an adaptable response in distinct pH and salt profiles, indicating that bacterial LiP was some halophilic type. Additionally, the strain AC149 produced an alkali and extreme halophilic lignin peroxidase, which are promising profiles for their future application under lignocellulosic biomass at bioethanol biorefineries.
650 $aActinomyces
650 $aCellulases
650 $aLaccase
650 $aLignin peroxidase
650 $aManganese peroxidase
650 $aActinomiceto
650 $aCelulase
650 $aPeroxidase
700 1 $aLIMA, L. M. dos S.
700 1 $aMARQUES, K. de M.
700 1 $aGUTIERRES, F. C.
700 1 $aGUERINI, G. G.
700 1 $aSILVEIRA, M. A. V.
700 1 $aFIGUEIREDO, G. M. de
700 1 $aVITAL, V. G.
700 1 $aROSWELL, M. R.
700 1 $aMELO, I. S. de
700 1 $aOKAMOTO, D. N.
700 1 $aVASCONCELLOS, S. P. de
773 $tArchives of Microbiology$gv. 206, n. 4, article 161, 2024.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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