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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
30/12/2024 |
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Data da última atualização: |
30/12/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PRADO, C. M. do; RODRIGUES, J. F. V.; FROTA, G. A.; VIEIRA, D. L.; MONTEIRO, J. P.; MOLENTO, M. B. |
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Afiliação: |
C. MELCHIOR DO PRADO, FEDERAL UNIVERSITY OF PARANA; J. FERREIRA VASCONCELOS RODRIGUES, FEDERAL UNIVERSITY OF PARANA; G. A. FROTA, STATE UNIVERSITY OF ACARAÚ VALLEY; D. L. VIEIRA, FEDERAL UNIVERSITY OF PARANA; JOMAR PATRICIO MONTEIRO, CNPC; M. BELTRÃO MOLENTO, FEDERAL UNIVERSITY OF PARANA. |
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Título: |
Genotyping of benzimidazole resistance using β-tubulin isotype 1 marker in Haemonchus contortus of sheep and goats in Paraná, Southern Brazil. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Journal of Helminthology, v. 98, e77, p. 1–8, 2024. |
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DOI: |
10.1017/S0022149X24000555 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract - Haemonchus contortus is one of the most pathogenic gastrointestinal parasites that infect small ruminants. The indiscriminate use of anthelmintics (i.e., benzimidazole class, BZ) to control infections has led to the reduction of drug efficacy in H. contortus populations worldwide. Resistance to BZ is associated with high frequencies of single nucleotide polymorphisms at F200Y, F167Y, and E198A positions of the β-tubulin isotype 1 gene. This study aimed to determine the frequency of single nucleotide polymorphisms associated with BZ resistance in H. contortus from 18 farms (545 sheep and 124 goats) in Paraná, Southern Brazil. Health management practices were identified as risk factors from individual farms. Genomic DNA was extracted from 20,000 larvae/farm and used in quantitative polymerase chain reaction assays for the three mutations. We ran a correlation analysis between flock health and quantitative polymerase chain reaction data. H. contortus was the most prevalent parasite in 67% (12/18) of the farms. Resistant allele frequencies were detected for F200Y (var. 46.4 to 72.0%) and F167Y (var. 15.7 to 23.8%). Only (100.0%) susceptible alleles were detected for the E198A. High treatment frequency (15/18), visual weight estimations for anthelmintic dose (15/18), no integration with other farm practices (14/18), treatment of all animals (14/18), and no quarantine period for newly acquired animals (10/18) were considered the most critical risk factors associated with BZ resistance. This is the first systematic prevalence study linking management practices on smallholder farms and the molecular data of BZ resistance of H. contortus in Southern Brazil. MenosAbstract - Haemonchus contortus is one of the most pathogenic gastrointestinal parasites that infect small ruminants. The indiscriminate use of anthelmintics (i.e., benzimidazole class, BZ) to control infections has led to the reduction of drug efficacy in H. contortus populations worldwide. Resistance to BZ is associated with high frequencies of single nucleotide polymorphisms at F200Y, F167Y, and E198A positions of the β-tubulin isotype 1 gene. This study aimed to determine the frequency of single nucleotide polymorphisms associated with BZ resistance in H. contortus from 18 farms (545 sheep and 124 goats) in Paraná, Southern Brazil. Health management practices were identified as risk factors from individual farms. Genomic DNA was extracted from 20,000 larvae/farm and used in quantitative polymerase chain reaction assays for the three mutations. We ran a correlation analysis between flock health and quantitative polymerase chain reaction data. H. contortus was the most prevalent parasite in 67% (12/18) of the farms. Resistant allele frequencies were detected for F200Y (var. 46.4 to 72.0%) and F167Y (var. 15.7 to 23.8%). Only (100.0%) susceptible alleles were detected for the E198A. High treatment frequency (15/18), visual weight estimations for anthelmintic dose (15/18), no integration with other farm practices (14/18), treatment of all animals (14/18), and no quarantine period for newly acquired animals (10/18) were considered the most critical risk factors associated wit... Mostrar Tudo |
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Palavras-Chave: |
Allelic mutations; Control strategies; Parasite infection. |
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Thesagro: |
Caprino; Haemonchus Contortus; Helminto Gastrintestinal; Ovino; Parasitologia; Parasitose. |
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Thesaurus Nal: |
Anthelmintics; Brazil; Drug resistance; Gastrointestinal nematodes; Genotyping; Goats; Helminths; Nematode infections; Parasites; Risk factors; Sheep; Small ruminants. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02973naa a2200445 a 4500
001 2171016
005 2024-12-30
008 2024 bl uuuu u00u1 u #d
024 7 $a10.1017/S0022149X24000555$2DOI
100 1 $aPRADO, C. M. do
245 $aGenotyping of benzimidazole resistance using β-tubulin isotype 1 marker in Haemonchus contortus of sheep and goats in Paraná, Southern Brazil.$h[electronic resource]
260 $c2024
520 $aAbstract - Haemonchus contortus is one of the most pathogenic gastrointestinal parasites that infect small ruminants. The indiscriminate use of anthelmintics (i.e., benzimidazole class, BZ) to control infections has led to the reduction of drug efficacy in H. contortus populations worldwide. Resistance to BZ is associated with high frequencies of single nucleotide polymorphisms at F200Y, F167Y, and E198A positions of the β-tubulin isotype 1 gene. This study aimed to determine the frequency of single nucleotide polymorphisms associated with BZ resistance in H. contortus from 18 farms (545 sheep and 124 goats) in Paraná, Southern Brazil. Health management practices were identified as risk factors from individual farms. Genomic DNA was extracted from 20,000 larvae/farm and used in quantitative polymerase chain reaction assays for the three mutations. We ran a correlation analysis between flock health and quantitative polymerase chain reaction data. H. contortus was the most prevalent parasite in 67% (12/18) of the farms. Resistant allele frequencies were detected for F200Y (var. 46.4 to 72.0%) and F167Y (var. 15.7 to 23.8%). Only (100.0%) susceptible alleles were detected for the E198A. High treatment frequency (15/18), visual weight estimations for anthelmintic dose (15/18), no integration with other farm practices (14/18), treatment of all animals (14/18), and no quarantine period for newly acquired animals (10/18) were considered the most critical risk factors associated with BZ resistance. This is the first systematic prevalence study linking management practices on smallholder farms and the molecular data of BZ resistance of H. contortus in Southern Brazil.
650 $aAnthelmintics
650 $aBrazil
650 $aDrug resistance
650 $aGastrointestinal nematodes
650 $aGenotyping
650 $aGoats
650 $aHelminths
650 $aNematode infections
650 $aParasites
650 $aRisk factors
650 $aSheep
650 $aSmall ruminants
650 $aCaprino
650 $aHaemonchus Contortus
650 $aHelminto Gastrintestinal
650 $aOvino
650 $aParasitologia
650 $aParasitose
653 $aAllelic mutations
653 $aControl strategies
653 $aParasite infection
700 1 $aRODRIGUES, J. F. V.
700 1 $aFROTA, G. A.
700 1 $aVIEIRA, D. L.
700 1 $aMONTEIRO, J. P.
700 1 $aMOLENTO, M. B.
773 $tJournal of Helminthology$gv. 98, e77, p. 1–8, 2024.
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