|
Registro Completo |
|
Biblioteca(s): |
Embrapa Gado de Leite. |
|
Data corrente: |
24/12/2024 |
|
Data da última atualização: |
24/12/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
NOGUEIRA, B. C. F.; HONORIO, N. T. de B. S.; SOUZA, P. E. de A.; SOARES, G. O.; CAMPOS, A. K.; MARTINS, M. F.; CARVALHO, W. A.; GASPAR, E. B. |
|
Afiliação: |
BARBARA CRISTINA FELIX NOGUEIRA, UNIVERSIDADE FEDERAL DE VIÇOSA; NICOLE TAFNES DE BRITO SILVA HONORIO, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; PAOLA EDUARDA DE ALMEIDA SOUZA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; GABRIELLE OLIVEIRA SOARES, UNIVERSIDADE FEDERAL DE VIÇOSA; ARTUR KANADANI CAMPOS, UNIVERSIDADE FEDERAL DE VIÇOSA; MARTA FONSECA MARTINS, CNPGL; WANESSA ARAUJO CARVALHO, CNPGL; EMANUELLE BALDO GASPAR, CNPGL. |
|
Título: |
Evidence of the efficiency of reverse vaccinology against bovine parasites: a systematic review. |
|
Ano de publicação: |
2024 |
|
Fonte/Imprenta: |
Acta Tropica, v. 260, 107478, 2024. |
|
DOI: |
https://doi.org/10.1016/j.actatropica.2024.107478 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Reverse vaccinology is a novel vaccine development technology that uses genome and proteome analyses through bioinformatics to select antigenic epitopes capable of eliciting an immunological and protective response through a quick and cheap methodology. However, data on its use in animal health are scant and further research is advocated. Therefore, this systematic review aimed to evaluate the evidence of the efficiency of reverse vaccinology in the search for antigens against bovine parasites, as well as its perspectives and limitations. One hundred seventy-four studies were found, of which 95 were selected for full reading following the PRISMA guidelines and considering all databases. After the last evaluation and reading of the references, only 19 studies were included and evaluated for methodological quality and biases. The studies applied reverse vaccinology to bacteria, protozoa, and ectoparasites that affect cattle, emphasizing on the tick species Rhipicephalus microplus and the protozoa of the genus Babesia that use it as a vector. Most studies evaluated the acquisition of an immune response through ELISA, WB and IFAT analyses to measure predominantly IgG. In addition, many studies did not examine the complete proteome of the parasites and are carried out only in silico, in vitro, or even with unrelated animals, the reason why they were excluded from our systematic review. Due to lack of studies that met the eligibility criteria, in this systematic review we also included studies carried out with different groups and species of parasites, providing a broad overview of the application of this technique in cattle farming. Conversely, this also resulted in variable methodologies, which makes comparison among studies difficult. Despite that, the application of reverse vaccinology in cattle farming has shown promising results in the development of immunological and protective responses in cattle. However, research methodologies need to be improved to reduce biases and obtain reliable results, in addition to clarity of data and methodologies to enable reproducibility. MenosReverse vaccinology is a novel vaccine development technology that uses genome and proteome analyses through bioinformatics to select antigenic epitopes capable of eliciting an immunological and protective response through a quick and cheap methodology. However, data on its use in animal health are scant and further research is advocated. Therefore, this systematic review aimed to evaluate the evidence of the efficiency of reverse vaccinology in the search for antigens against bovine parasites, as well as its perspectives and limitations. One hundred seventy-four studies were found, of which 95 were selected for full reading following the PRISMA guidelines and considering all databases. After the last evaluation and reading of the references, only 19 studies were included and evaluated for methodological quality and biases. The studies applied reverse vaccinology to bacteria, protozoa, and ectoparasites that affect cattle, emphasizing on the tick species Rhipicephalus microplus and the protozoa of the genus Babesia that use it as a vector. Most studies evaluated the acquisition of an immune response through ELISA, WB and IFAT analyses to measure predominantly IgG. In addition, many studies did not examine the complete proteome of the parasites and are carried out only in silico, in vitro, or even with unrelated animals, the reason why they were excluded from our systematic review. Due to lack of studies that met the eligibility criteria, in this systematic review we also inc... Mostrar Tudo |
|
Palavras-Chave: |
Bioinformática; Epítopo; Parasita; Resposta imune. |
|
Thesagro: |
Antígeno; Bovino; Vacina. |
|
Thesaurus Nal: |
Antigens; Bioinformatics; Epitopes; Immune response; Vaccines. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 03111naa a2200361 a 4500
001 2170733
005 2024-12-24
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.actatropica.2024.107478$2DOI
100 1 $aNOGUEIRA, B. C. F.
245 $aEvidence of the efficiency of reverse vaccinology against bovine parasites$ba systematic review.$h[electronic resource]
260 $c2024
520 $aReverse vaccinology is a novel vaccine development technology that uses genome and proteome analyses through bioinformatics to select antigenic epitopes capable of eliciting an immunological and protective response through a quick and cheap methodology. However, data on its use in animal health are scant and further research is advocated. Therefore, this systematic review aimed to evaluate the evidence of the efficiency of reverse vaccinology in the search for antigens against bovine parasites, as well as its perspectives and limitations. One hundred seventy-four studies were found, of which 95 were selected for full reading following the PRISMA guidelines and considering all databases. After the last evaluation and reading of the references, only 19 studies were included and evaluated for methodological quality and biases. The studies applied reverse vaccinology to bacteria, protozoa, and ectoparasites that affect cattle, emphasizing on the tick species Rhipicephalus microplus and the protozoa of the genus Babesia that use it as a vector. Most studies evaluated the acquisition of an immune response through ELISA, WB and IFAT analyses to measure predominantly IgG. In addition, many studies did not examine the complete proteome of the parasites and are carried out only in silico, in vitro, or even with unrelated animals, the reason why they were excluded from our systematic review. Due to lack of studies that met the eligibility criteria, in this systematic review we also included studies carried out with different groups and species of parasites, providing a broad overview of the application of this technique in cattle farming. Conversely, this also resulted in variable methodologies, which makes comparison among studies difficult. Despite that, the application of reverse vaccinology in cattle farming has shown promising results in the development of immunological and protective responses in cattle. However, research methodologies need to be improved to reduce biases and obtain reliable results, in addition to clarity of data and methodologies to enable reproducibility.
650 $aAntigens
650 $aBioinformatics
650 $aEpitopes
650 $aImmune response
650 $aVaccines
650 $aAntígeno
650 $aBovino
650 $aVacina
653 $aBioinformática
653 $aEpítopo
653 $aParasita
653 $aResposta imune
700 1 $aHONORIO, N. T. de B. S.
700 1 $aSOUZA, P. E. de A.
700 1 $aSOARES, G. O.
700 1 $aCAMPOS, A. K.
700 1 $aMARTINS, M. F.
700 1 $aCARVALHO, W. A.
700 1 $aGASPAR, E. B.
773 $tActa Tropica$gv. 260, 107478, 2024.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
