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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
12/12/2024 |
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Data da última atualização: |
07/01/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PEREIRA, H. P.; ARCURI, E. F.; OLIVEIRA, F. R. de; CARVALHO, C. V. de; HONORIO, N. T. de B. S.; GASPAR, E. B.; REIS, D. R. de L.; DOMINGUES, R.; BORGES, C. A. V.; SOUZA, G. N. de; LANGE, C. C.; MARTINS, M. F. |
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Afiliação: |
HYAGO PASSE PEREIRA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; EDNA FROEDER ARCURI, CNPGL; FERNANDO RODRIGUES DE OLIVEIRA; CLARISSA VIDAL DE CARVALHO, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; NICOLE TAFNES DE BRITO SILVA HONORIO, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; EMANUELLE BALDO GASPAR, CNPGL; DANIELE RIBEIRO DE LIMA REIS FAZA, CNPGL; ROBERT DOMINGUES, CNPGL; CRISTIANO AMANCIO VIEIRA BORGES, CNPGL; GUILHERME NUNES DE SOUZA, CNPGL; CARLA CHRISTINE LANGE, CNPGL; MARTA FONSECA MARTINS, CNPGL. |
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Título: |
Evaluation and characterization of lytic phages and their recombinant endolysins for control of Staphylococcus aureus aiming to mitigate bovine mastitis. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Microbial Pathogenesis, v. 199, 107188, 2025. |
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DOI: |
https://doi.org/10.1016/j.micpath.2024.107188 |
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Idioma: |
Inglês |
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Conteúdo: |
As a natural alternative to conventional antimicrobials, bacteriophages are emerging as highly effective biocontrol agents against Staphylococcus aureus and other pathogenic bacteria. Due to the wide diversity of S. aureus types and the emergence of antibiotic-resistant strains, the search for highly lytic and prevalent bac teriophages against S. aureus is justified. In this study, we sought to characterized the lytic Phage 2 and Phage 4 biologically and morphologically and their recombinant endolysins EndF2 and EndF4. Transmission electron microscopy analysis revealed that these phages exhibited a structure with a polyhedral head and non-contractile tail, typical characteristics of the Siphoviridae family. Host spectrum identification showed that Phage 2 lysed 62.2 % (N = 90) of S. aureus strains and Phage 4 lysed 51.1 % (N = 90). In vitro tests with extracorporeal cow teats indicated that Phage 2 reduced the S. aureus load by up to 78.7 %. Furthermore, recombinant endolysins EndF2 and EndF4 have catalytic and recognition/binding domains in their structures related to lytic activity, and both endolysins do not present critical aspects of allergenicity. Furthermore, EndF2 lysed 71.4 % (N = 42) and EndF4 lysed 76.2 % (N = 42) of the S. aureus strains tested. These findings indicate that Phages 2 and 4 and their recombinant endolysins EndF2 and EndF4 could potentially be used as tools for the prevention and control of S. aureus, suggesting they are potentially valuable biocontrol agents to mitigate the spread of S. aureus in the dairy industries and production chain. MenosAs a natural alternative to conventional antimicrobials, bacteriophages are emerging as highly effective biocontrol agents against Staphylococcus aureus and other pathogenic bacteria. Due to the wide diversity of S. aureus types and the emergence of antibiotic-resistant strains, the search for highly lytic and prevalent bac teriophages against S. aureus is justified. In this study, we sought to characterized the lytic Phage 2 and Phage 4 biologically and morphologically and their recombinant endolysins EndF2 and EndF4. Transmission electron microscopy analysis revealed that these phages exhibited a structure with a polyhedral head and non-contractile tail, typical characteristics of the Siphoviridae family. Host spectrum identification showed that Phage 2 lysed 62.2 % (N = 90) of S. aureus strains and Phage 4 lysed 51.1 % (N = 90). In vitro tests with extracorporeal cow teats indicated that Phage 2 reduced the S. aureus load by up to 78.7 %. Furthermore, recombinant endolysins EndF2 and EndF4 have catalytic and recognition/binding domains in their structures related to lytic activity, and both endolysins do not present critical aspects of allergenicity. Furthermore, EndF2 lysed 71.4 % (N = 42) and EndF4 lysed 76.2 % (N = 42) of the S. aureus strains tested. These findings indicate that Phages 2 and 4 and their recombinant endolysins EndF2 and EndF4 could potentially be used as tools for the prevention and control of S. aureus, suggesting they are potentially valuable biocont... Mostrar Tudo |
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Thesagro: |
Bactéria; Bovino; Doença Animal; Proteína. |
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Thesaurus Nal: |
Bacteriophages; Biopharmaceuticals; Pathogens; Phage therapy; Recombinant proteins. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02728naa a2200373 a 4500
001 2170566
005 2025-01-07
008 2025 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.micpath.2024.107188$2DOI
100 1 $aPEREIRA, H. P.
245 $aEvaluation and characterization of lytic phages and their recombinant endolysins for control of Staphylococcus aureus aiming to mitigate bovine mastitis.$h[electronic resource]
260 $c2025
520 $aAs a natural alternative to conventional antimicrobials, bacteriophages are emerging as highly effective biocontrol agents against Staphylococcus aureus and other pathogenic bacteria. Due to the wide diversity of S. aureus types and the emergence of antibiotic-resistant strains, the search for highly lytic and prevalent bac teriophages against S. aureus is justified. In this study, we sought to characterized the lytic Phage 2 and Phage 4 biologically and morphologically and their recombinant endolysins EndF2 and EndF4. Transmission electron microscopy analysis revealed that these phages exhibited a structure with a polyhedral head and non-contractile tail, typical characteristics of the Siphoviridae family. Host spectrum identification showed that Phage 2 lysed 62.2 % (N = 90) of S. aureus strains and Phage 4 lysed 51.1 % (N = 90). In vitro tests with extracorporeal cow teats indicated that Phage 2 reduced the S. aureus load by up to 78.7 %. Furthermore, recombinant endolysins EndF2 and EndF4 have catalytic and recognition/binding domains in their structures related to lytic activity, and both endolysins do not present critical aspects of allergenicity. Furthermore, EndF2 lysed 71.4 % (N = 42) and EndF4 lysed 76.2 % (N = 42) of the S. aureus strains tested. These findings indicate that Phages 2 and 4 and their recombinant endolysins EndF2 and EndF4 could potentially be used as tools for the prevention and control of S. aureus, suggesting they are potentially valuable biocontrol agents to mitigate the spread of S. aureus in the dairy industries and production chain.
650 $aBacteriophages
650 $aBiopharmaceuticals
650 $aPathogens
650 $aPhage therapy
650 $aRecombinant proteins
650 $aBactéria
650 $aBovino
650 $aDoença Animal
650 $aProteína
700 1 $aARCURI, E. F.
700 1 $aOLIVEIRA, F. R. de
700 1 $aCARVALHO, C. V. de
700 1 $aHONORIO, N. T. de B. S.
700 1 $aGASPAR, E. B.
700 1 $aREIS, D. R. de L.
700 1 $aDOMINGUES, R.
700 1 $aBORGES, C. A. V.
700 1 $aSOUZA, G. N. de
700 1 $aLANGE, C. C.
700 1 $aMARTINS, M. F.
773 $tMicrobial Pathogenesis$gv. 199, 107188, 2025.
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Embrapa Gado de Leite (CNPGL) |
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| 1. |  | FARIA, J. C.; VILARINHO, R. M. Detecção, clonagem parcial e sequenciamento de um begomovirus de soja perene. Fitopatologia Brasileira, v. 30, p. S184, ago. 2005. Suplemento, ref. 772. Edição de Resumos do XXXVIII Congresso Brasileiro de Fitopatologia, Brasília, DF, ago. 2005.| Tipo: Resumo em Anais de Congresso |
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