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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
18/11/2024 |
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Data da última atualização: |
18/11/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FREITAS, M. S. e; GARCÍA, H. D. M.; FIALA-RECHSTEINER, S. M. da E.; FONTES, C. S.; TEIXEIRA, C. S.; VIANA, J. H. M.; ALBINO, M. V.; COSTA, L. M. L. da; CARNEIRO, G. F.; RIBEIRO, G. C.; SANCLER-SILVA, Y. F. R. |
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Afiliação: |
MARCELA SOUZA E FREITAS, UNIVERSIDADE FEDERAL DE VIÇOSA; HENRY DAVID MOGOLLÓN GARCÍA, UNIVERSIDADE ESTADUAL DE CAMPINAS; SANDRA MARA DA ENCARNAÇÃO FIALA-RECHSTEINER, UNIVERSIDADE FEDERAL DE PELOTAS; CAROLINA SILVEIRA FONTES, UNIVERSIDADE FEDERAL DE VIÇOSA; CRISTIAN SILVA TEIXEIRA, UNIVERSIDADE FEDERAL DE VIÇOSA; JOAO HENRIQUE MOREIRA VIANA, CENARGEN; MATHEUS VILELA ALBINO, UNIVERSIDADE FEDERAL DE VIÇOSA; LORRAINE MARCELE LOPES DA COSTA, UNIVERSIDADE FEDERAL DE VIÇOSA; GUSTAVO FERRER CARNEIRO, UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO; GABRIELLA COSTA RIBEIRO, UNIVERSIDADE ESTADUAL PAULISTA; YAME FABRES ROBAINA SANCLER-SILVA, UNIVERSIDADE FEDERAL DE VIÇOSA. |
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Título: |
Is platelet-rich plasma effective in treating uterine inflammation in jennies inseminated with cryopreserved donkey semen? |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Theriogenology, v. 231, p. 144-153, 2025 |
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DOI: |
https://doi.org/10.1016/j.theriogenology.2024.10.009 |
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Idioma: |
Inglês |
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Conteúdo: |
Despite frozen donkey semen demonstrating high quality after thawing and achieving suitable pregnancy rates in mares, it yields unsatisfactory results in jennies, likely due to a stronger uterine inflammatory response. This study assessed the effects of platelet-rich plasma (PRP) on uterine inflammation and pregnancy rates in jennies inseminated with frozen donkey semen. Estrous cycles from 11 jennies were assigned to three groups: Control (CTR, n = 22) with no treatment; Single PRP infusion (S-PRP, n = 22) administered 30 h after ovulation induction, prior to artificial insemination (AI); and Double PRP infusion (D-PRP, n = 21) with the first infusion at 30 h after ovulation induction and the second 4 h after AI. Insemination was performed with frozen donkey semen (1 billion sperm) deposited deeply in the uterine horn immediately after ovulation. Endometrial edema, intrauterine fluid (IUF), uterine vascularization, and endometrial cytology were evaluated pre-AI (TCt) and post-AI (6, 24, and 48 h). Uterine biopsies were taken at T48 for histopathological and collagen evaluation. Peripheral blood samples were collected on D5 for serum progesterone measurement, and pregnancy was evaluated via ultrasonography on D14. Data were analyzed using GLMMs, ANOVA, Friedman, and Kruskal-Wallis tests in SAS and GraphPad Prism, with significance set at p < 0.05. The S-PRP group showed less IUF accumulation than the CTR group at T6. Other parameters showed no significant differences among the groups. Cytology revealed a high percentage of inflammatory cells at T6 in all groups, which decreased in subsequent evaluations. In the CTR group, neutrophil percentages were similar to TCt at T24, while treated groups reached this similarity only by T48. Eosinophil percentages increased over time only in the treated groups. Pregnancy rates showed no differences among the groups (CTR: 0 %, S-PRP: 0 %, D-PRP: 10 %). Results indicate that PRP treatments were ineffective in modulating uterine inflammation and did not enhance pregnancy rates in jennies inseminated with frozen donkey semen. MenosDespite frozen donkey semen demonstrating high quality after thawing and achieving suitable pregnancy rates in mares, it yields unsatisfactory results in jennies, likely due to a stronger uterine inflammatory response. This study assessed the effects of platelet-rich plasma (PRP) on uterine inflammation and pregnancy rates in jennies inseminated with frozen donkey semen. Estrous cycles from 11 jennies were assigned to three groups: Control (CTR, n = 22) with no treatment; Single PRP infusion (S-PRP, n = 22) administered 30 h after ovulation induction, prior to artificial insemination (AI); and Double PRP infusion (D-PRP, n = 21) with the first infusion at 30 h after ovulation induction and the second 4 h after AI. Insemination was performed with frozen donkey semen (1 billion sperm) deposited deeply in the uterine horn immediately after ovulation. Endometrial edema, intrauterine fluid (IUF), uterine vascularization, and endometrial cytology were evaluated pre-AI (TCt) and post-AI (6, 24, and 48 h). Uterine biopsies were taken at T48 for histopathological and collagen evaluation. Peripheral blood samples were collected on D5 for serum progesterone measurement, and pregnancy was evaluated via ultrasonography on D14. Data were analyzed using GLMMs, ANOVA, Friedman, and Kruskal-Wallis tests in SAS and GraphPad Prism, with significance set at p < 0.05. The S-PRP group showed less IUF accumulation than the CTR group at T6. Other parameters showed no significant differences among t... Mostrar Tudo |
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Palavras-Chave: |
Fertility; Frozen semen; Jackass; PRP. |
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Thesaurus Nal: |
Endometritis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03054naa a2200313 a 4500
001 2169227
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024 7 $ahttps://doi.org/10.1016/j.theriogenology.2024.10.009$2DOI
100 1 $aFREITAS, M. S. e
245 $aIs platelet-rich plasma effective in treating uterine inflammation in jennies inseminated with cryopreserved donkey semen?$h[electronic resource]
260 $c2025
520 $aDespite frozen donkey semen demonstrating high quality after thawing and achieving suitable pregnancy rates in mares, it yields unsatisfactory results in jennies, likely due to a stronger uterine inflammatory response. This study assessed the effects of platelet-rich plasma (PRP) on uterine inflammation and pregnancy rates in jennies inseminated with frozen donkey semen. Estrous cycles from 11 jennies were assigned to three groups: Control (CTR, n = 22) with no treatment; Single PRP infusion (S-PRP, n = 22) administered 30 h after ovulation induction, prior to artificial insemination (AI); and Double PRP infusion (D-PRP, n = 21) with the first infusion at 30 h after ovulation induction and the second 4 h after AI. Insemination was performed with frozen donkey semen (1 billion sperm) deposited deeply in the uterine horn immediately after ovulation. Endometrial edema, intrauterine fluid (IUF), uterine vascularization, and endometrial cytology were evaluated pre-AI (TCt) and post-AI (6, 24, and 48 h). Uterine biopsies were taken at T48 for histopathological and collagen evaluation. Peripheral blood samples were collected on D5 for serum progesterone measurement, and pregnancy was evaluated via ultrasonography on D14. Data were analyzed using GLMMs, ANOVA, Friedman, and Kruskal-Wallis tests in SAS and GraphPad Prism, with significance set at p < 0.05. The S-PRP group showed less IUF accumulation than the CTR group at T6. Other parameters showed no significant differences among the groups. Cytology revealed a high percentage of inflammatory cells at T6 in all groups, which decreased in subsequent evaluations. In the CTR group, neutrophil percentages were similar to TCt at T24, while treated groups reached this similarity only by T48. Eosinophil percentages increased over time only in the treated groups. Pregnancy rates showed no differences among the groups (CTR: 0 %, S-PRP: 0 %, D-PRP: 10 %). Results indicate that PRP treatments were ineffective in modulating uterine inflammation and did not enhance pregnancy rates in jennies inseminated with frozen donkey semen.
650 $aEndometritis
653 $aFertility
653 $aFrozen semen
653 $aJackass
653 $aPRP
700 1 $aGARCÍA, H. D. M.
700 1 $aFIALA-RECHSTEINER, S. M. da E.
700 1 $aFONTES, C. S.
700 1 $aTEIXEIRA, C. S.
700 1 $aVIANA, J. H. M.
700 1 $aALBINO, M. V.
700 1 $aCOSTA, L. M. L. da
700 1 $aCARNEIRO, G. F.
700 1 $aRIBEIRO, G. C.
700 1 $aSANCLER-SILVA, Y. F. R.
773 $tTheriogenology$gv. 231, p. 144-153, 2025
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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