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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
22/10/2024 |
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Data da última atualização: |
11/11/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
AYMÉE, L.; BORGES, A. L. dos S. B.; SOUZA, G. N. de; LILENBAUM, W. |
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Afiliação: |
LUIZA AYMÉE, UNIVERSIDADE FEDERAL FLUMINENSE; ANA LUIZA DOS SANTOS BAPTISTA BORGES, UNIVERSIDADE FEDERAL FLUMINENSE; GUILHERME NUNES DE SOUZA, CNPGL; WALTER LILENBAUM, UNIVERSIDADE FEDERAL FLUMINENSE. |
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Título: |
Is microscopic agglutination test a reliable method for diagnosing the bovine genital leptospirosis syndrome? |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Veterinary Research Communications, v. 48, p. 4185-4189, 2024. |
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DOI: |
https://doi.org/10.1007/s11259-024-10560-4 |
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Idioma: |
Inglês |
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Conteúdo: |
Bovine Genital Leptospirosis (BGL) is a chronic reproductive syndrome characterized by genital infection by Leptospira spp. An accurate diagnosis of BGL is crucial to implementing proper control measures in field conditions. This study aimed to evaluate the reliability of serology by Microscopic Agglutination Test (MAT) for diagnosing leptospirosis in subfertile cows with genital infection. Of three herds, 93 non-pregnant cows with reproductive failures were submitted to the blood sampling (serology by MAT) and genital samples (lipL32-PCR). A total of 62/93 (66.6%) cows presented seroreactive to cutoff 100, while 45/93 (48.4%) cows were positive to cutoff 200, mainly against the Sejroe serogroup. In PCR analysis, 55/93 (59.1%) were positive. MAT results were compared with PCR (considered the standard), and test parameters and Cohen’s kappa (ƙ) were calculated for the cut-offs 100 and 200. A ROC curve was performed for each cut-off of titers 100 to 1,600. The sensitivity and specificity of MAT100 were calculated at 66.6% and 33.3%, while for MAT200 the sensitivity was estimated as 35% and specificity as 54.5%. The accuracy of MAT was poor, being 54.8% in MAT100 and 42% in MAT200. Furthermore, the area under the curve of ROC analysis was low for all titers, and the correlation was poor for MAT100 and MAT200 (ƙ < 0). The results demonstrated that MAT is a limited technique to diagnose bovine genital carriers individually, and if only MAT is applied, genital carriers may pass undetected, impairing the control programs MenosBovine Genital Leptospirosis (BGL) is a chronic reproductive syndrome characterized by genital infection by Leptospira spp. An accurate diagnosis of BGL is crucial to implementing proper control measures in field conditions. This study aimed to evaluate the reliability of serology by Microscopic Agglutination Test (MAT) for diagnosing leptospirosis in subfertile cows with genital infection. Of three herds, 93 non-pregnant cows with reproductive failures were submitted to the blood sampling (serology by MAT) and genital samples (lipL32-PCR). A total of 62/93 (66.6%) cows presented seroreactive to cutoff 100, while 45/93 (48.4%) cows were positive to cutoff 200, mainly against the Sejroe serogroup. In PCR analysis, 55/93 (59.1%) were positive. MAT results were compared with PCR (considered the standard), and test parameters and Cohen’s kappa (ƙ) were calculated for the cut-offs 100 and 200. A ROC curve was performed for each cut-off of titers 100 to 1,600. The sensitivity and specificity of MAT100 were calculated at 66.6% and 33.3%, while for MAT200 the sensitivity was estimated as 35% and specificity as 54.5%. The accuracy of MAT was poor, being 54.8% in MAT100 and 42% in MAT200. Furthermore, the area under the curve of ROC analysis was low for all titers, and the correlation was poor for MAT100 and MAT200 (ƙ < 0). The results demonstrated that MAT is a limited technique to diagnose bovine genital carriers individually, and if only MAT is applied, genital carriers may pass un... Mostrar Tudo |
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Palavras-Chave: |
Especificidade; PCR; Sensibilidade; Sensitivity; Specificity. |
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Thesagro: |
Bovino; Doença Animal; Leptospirose. |
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Thesaurus Nal: |
Dairy cattle; Leptospira. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02402naa a2200289 a 4500
001 2168361
005 2024-11-11
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s11259-024-10560-4$2DOI
100 1 $aAYMÉE, L.
245 $aIs microscopic agglutination test a reliable method for diagnosing the bovine genital leptospirosis syndrome?$h[electronic resource]
260 $c2024
520 $aBovine Genital Leptospirosis (BGL) is a chronic reproductive syndrome characterized by genital infection by Leptospira spp. An accurate diagnosis of BGL is crucial to implementing proper control measures in field conditions. This study aimed to evaluate the reliability of serology by Microscopic Agglutination Test (MAT) for diagnosing leptospirosis in subfertile cows with genital infection. Of three herds, 93 non-pregnant cows with reproductive failures were submitted to the blood sampling (serology by MAT) and genital samples (lipL32-PCR). A total of 62/93 (66.6%) cows presented seroreactive to cutoff 100, while 45/93 (48.4%) cows were positive to cutoff 200, mainly against the Sejroe serogroup. In PCR analysis, 55/93 (59.1%) were positive. MAT results were compared with PCR (considered the standard), and test parameters and Cohen’s kappa (ƙ) were calculated for the cut-offs 100 and 200. A ROC curve was performed for each cut-off of titers 100 to 1,600. The sensitivity and specificity of MAT100 were calculated at 66.6% and 33.3%, while for MAT200 the sensitivity was estimated as 35% and specificity as 54.5%. The accuracy of MAT was poor, being 54.8% in MAT100 and 42% in MAT200. Furthermore, the area under the curve of ROC analysis was low for all titers, and the correlation was poor for MAT100 and MAT200 (ƙ < 0). The results demonstrated that MAT is a limited technique to diagnose bovine genital carriers individually, and if only MAT is applied, genital carriers may pass undetected, impairing the control programs
650 $aDairy cattle
650 $aLeptospira
650 $aBovino
650 $aDoença Animal
650 $aLeptospirose
653 $aEspecificidade
653 $aPCR
653 $aSensibilidade
653 $aSensitivity
653 $aSpecificity
700 1 $aBORGES, A. L. dos S. B.
700 1 $aSOUZA, G. N. de
700 1 $aLILENBAUM, W.
773 $tVeterinary Research Communications$gv. 48, p. 4185-4189, 2024.
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| 4. |  | LUNZ, A. M.; REIS, C. A. F. Insetos. In: REIS, C. F.; OLIVEIRA, E. B. de; SANTOS, A. M. (Ed.). Mogno-africano (Khaya spp.): atualidades e perspectivas do cultivo no Brasil. Brasília, DF: Embrapa, 2019. Cap. 10, p. 252-265.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Florestas. |
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| 13. | | AGUIAR, N. S. de; HANSEL, F. A.; REIS, C. A. F.; LAZZAROTTO, M.; WENDLING, I. Optimizing the Vanillin‐Acid Sulfuric method to total saponin content in leaves of yerba mate clones. Chemistry & Biodiversity, v. 21, n. 4, e202301883, p. 1-10, 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
| Biblioteca(s): Embrapa Florestas; Embrapa Uva e Vinho. |
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| Registros recuperados : 61 | |
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