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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
20/08/2024 |
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Data da última atualização: |
20/08/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, C. M. dos; NASCIMENTO, W. B. A.; CESAR, M. J. S. C.; BALDANI, J. I.; SCHWAB, S. |
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Afiliação: |
CARLOS M. DOS SANTOS, UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO; W. BRUNO A. NASCIMENTO, UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO; M. JOANA S. C. CESAR, UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO; JOSE IVO BALDANI, CNPAB; STEFAN SCHWAB, CNPAB. |
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Título: |
Diversity of bacteria of the genus Sphingomonas associated with sugarcane (Saccharum spp.) culm apoplast fuid and their agrotechnological potential. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
World Journal of Microbiology and Biotechnology, v. 40, n. 304, Published online August, 2024. |
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ISSN: |
0959-3993 |
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DOI: |
10.1007/s11274-024-04111-x |
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Idioma: |
Inglês |
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Conteúdo: |
In sugarcane, sequences related to the genus Sphingomonas have been widely detected by microbiome studies. In this work, the presence of bacteria of this genus was confrmed using culture-dependent and independent techniques. A collection of thirty isolates was obtained using semispecifc cultivation conditions, and a specifc PCR assay was applied to help confrm the isolates as belonging to the genus. A series of laboratory evaluations were carried out to identify potential properties among the isolates in the collection, which consequently allowed the identifcation of some most promising isolates for the development of new agricultural bioinputs. In a separate analysis, the culture-independent fuorescence in situ hybridization (FISH) methodology was applied to demonstrate the natural occurrence of Sphingomonas in diferent organs and tissues of sugarcane. The results showed the presence of bacteria of the genus in the spaces between cells (apoplast) of the culm parenchyma, in vessels in the region of the leaf vein, on the adaxial surface of the leaf blade, and on the root surface, sometimes close to the base of root hairs, which suggests extensive colonization on the host plant. In summary, the present study corroborates previous metagenomic amplicon sequencing results that indicated a high occurrence of Sphingomonas associated with sugarcane. This is the frst study that uses approaches other than amplicon sequencing to confrm the occurrence of the genus in sugarcane and, at the same time, demonstrates potentially benefcial activities to be explored by sugarcane cultivation. MenosIn sugarcane, sequences related to the genus Sphingomonas have been widely detected by microbiome studies. In this work, the presence of bacteria of this genus was confrmed using culture-dependent and independent techniques. A collection of thirty isolates was obtained using semispecifc cultivation conditions, and a specifc PCR assay was applied to help confrm the isolates as belonging to the genus. A series of laboratory evaluations were carried out to identify potential properties among the isolates in the collection, which consequently allowed the identifcation of some most promising isolates for the development of new agricultural bioinputs. In a separate analysis, the culture-independent fuorescence in situ hybridization (FISH) methodology was applied to demonstrate the natural occurrence of Sphingomonas in diferent organs and tissues of sugarcane. The results showed the presence of bacteria of the genus in the spaces between cells (apoplast) of the culm parenchyma, in vessels in the region of the leaf vein, on the adaxial surface of the leaf blade, and on the root surface, sometimes close to the base of root hairs, which suggests extensive colonization on the host plant. In summary, the present study corroborates previous metagenomic amplicon sequencing results that indicated a high occurrence of Sphingomonas associated with sugarcane. This is the frst study that uses approaches other than amplicon sequencing to confrm the occurrence of the genus in sugarcane and, at t... Mostrar Tudo |
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Palavras-Chave: |
Agricultural bioinputs; Plant growth promoting rhizobacteria. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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