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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
20/08/2024 |
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Data da última atualização: |
20/08/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, C. M. dos; NASCIMENTO, W. B. A.; CESAR, M. J. S. C.; BALDANI, J. I.; SCHWAB, S. |
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Afiliação: |
CARLOS M. DOS SANTOS, UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO; W. BRUNO A. NASCIMENTO, UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO; M. JOANA S. C. CESAR, UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO; JOSE IVO BALDANI, CNPAB; STEFAN SCHWAB, CNPAB. |
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Título: |
Diversity of bacteria of the genus Sphingomonas associated with sugarcane (Saccharum spp.) culm apoplast fluid and their agrotechnological potential. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
World Journal of Microbiology and Biotechnology, v. 40, n. 304, Published online August, 2024. |
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ISSN: |
0959-3993 |
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DOI: |
10.1007/s11274-024-04111-x |
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Idioma: |
Inglês |
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Conteúdo: |
In sugarcane, sequences related to the genus Sphingomonas have been widely detected by microbiome studies. In this work, the presence of bacteria of this genus was confrmed using culture-dependent and independent techniques. A collection of thirty isolates was obtained using semispecifc cultivation conditions, and a specifc PCR assay was applied to help confrm the isolates as belonging to the genus. A series of laboratory evaluations were carried out to identify potential properties among the isolates in the collection, which consequently allowed the identifcation of some most promising isolates for the development of new agricultural bioinputs. In a separate analysis, the culture-independent fuorescence in situ hybridization (FISH) methodology was applied to demonstrate the natural occurrence of Sphingomonas in diferent organs and tissues of sugarcane. The results showed the presence of bacteria of the genus in the spaces between cells (apoplast) of the culm parenchyma, in vessels in the region of the leaf vein, on the adaxial surface of the leaf blade, and on the root surface, sometimes close to the base of root hairs, which suggests extensive colonization on the host plant. In summary, the present study corroborates previous metagenomic amplicon sequencing results that indicated a high occurrence of Sphingomonas associated with sugarcane. This is the frst study that uses approaches other than amplicon sequencing to confrm the occurrence of the genus in sugarcane and, at the same time, demonstrates potentially benefcial activities to be explored by sugarcane cultivation. MenosIn sugarcane, sequences related to the genus Sphingomonas have been widely detected by microbiome studies. In this work, the presence of bacteria of this genus was confrmed using culture-dependent and independent techniques. A collection of thirty isolates was obtained using semispecifc cultivation conditions, and a specifc PCR assay was applied to help confrm the isolates as belonging to the genus. A series of laboratory evaluations were carried out to identify potential properties among the isolates in the collection, which consequently allowed the identifcation of some most promising isolates for the development of new agricultural bioinputs. In a separate analysis, the culture-independent fuorescence in situ hybridization (FISH) methodology was applied to demonstrate the natural occurrence of Sphingomonas in diferent organs and tissues of sugarcane. The results showed the presence of bacteria of the genus in the spaces between cells (apoplast) of the culm parenchyma, in vessels in the region of the leaf vein, on the adaxial surface of the leaf blade, and on the root surface, sometimes close to the base of root hairs, which suggests extensive colonization on the host plant. In summary, the present study corroborates previous metagenomic amplicon sequencing results that indicated a high occurrence of Sphingomonas associated with sugarcane. This is the frst study that uses approaches other than amplicon sequencing to confrm the occurrence of the genus in sugarcane and, at t... Mostrar Tudo |
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Palavras-Chave: |
Agricultural bioinputs; Plant growth promoting rhizobacteria. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02389naa a2200217 a 4500
001 2166645
005 2024-08-20
008 2024 bl uuuu u00u1 u #d
022 $a0959-3993
024 7 $a10.1007/s11274-024-04111-x$2DOI
100 1 $aSANTOS, C. M. dos
245 $aDiversity of bacteria of the genus Sphingomonas associated with sugarcane (Saccharum spp.) culm apoplast fluid and their agrotechnological potential.$h[electronic resource]
260 $c2024
520 $aIn sugarcane, sequences related to the genus Sphingomonas have been widely detected by microbiome studies. In this work, the presence of bacteria of this genus was confrmed using culture-dependent and independent techniques. A collection of thirty isolates was obtained using semispecifc cultivation conditions, and a specifc PCR assay was applied to help confrm the isolates as belonging to the genus. A series of laboratory evaluations were carried out to identify potential properties among the isolates in the collection, which consequently allowed the identifcation of some most promising isolates for the development of new agricultural bioinputs. In a separate analysis, the culture-independent fuorescence in situ hybridization (FISH) methodology was applied to demonstrate the natural occurrence of Sphingomonas in diferent organs and tissues of sugarcane. The results showed the presence of bacteria of the genus in the spaces between cells (apoplast) of the culm parenchyma, in vessels in the region of the leaf vein, on the adaxial surface of the leaf blade, and on the root surface, sometimes close to the base of root hairs, which suggests extensive colonization on the host plant. In summary, the present study corroborates previous metagenomic amplicon sequencing results that indicated a high occurrence of Sphingomonas associated with sugarcane. This is the frst study that uses approaches other than amplicon sequencing to confrm the occurrence of the genus in sugarcane and, at the same time, demonstrates potentially benefcial activities to be explored by sugarcane cultivation.
653 $aAgricultural bioinputs
653 $aPlant growth promoting rhizobacteria
700 1 $aNASCIMENTO, W. B. A.
700 1 $aCESAR, M. J. S. C.
700 1 $aBALDANI, J. I.
700 1 $aSCHWAB, S.
773 $tWorld Journal of Microbiology and Biotechnology$gv. 40, n. 304, Published online August, 2024.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Biblioteca |
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| Registros recuperados : 25 | |
| 6. |  | ROSSATO, M.; SANTIAGO, T. R.; ALVES, A. R.; CARVALHO, I. C. B.; LOPES, C. A. Reaction of chickpea cultivars to bacterial wilt, a new disease to a crop under expansion in Brazil. In: INTERNATIONAL BACTERIAL WILT SYMPOSIUM, 7., 2023, Montevideo. Abstract book. Montevideo: Universidad de la República Uruguay : INIA, 2023. p. 104.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
|    |
| 9. | | SANTIAGO, T. R.; DUARTE, K. E.; SOUZA, W. R. de; STEINDORFF, A. S.; FORMIGHIERI, E. F.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Divergence of DNA methylation and gene expression in Setaria viridis accessions under drought stress. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 5., 2018, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2018. p. 31.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 10. | | PINTO, K. L. M.; MENDES, B. L.; RIBEIRO, L. M.; BARROS, S. A. M.; SANTIAGO, T. R.; SILVA, L. P. da. Controle de Pratylenchus zeae e promoção de crescimento vegetal por nanopartículas de cobre obtidas por síntese verde. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 54., 2025, Lavras. Anais do evento. Brasília, DF: Sociedade Brasileira de Fitopatologia, 2025. p. 756 CBFITO 2025. Na publicação: Luciano Paulino Silva.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 11. | | RÊGO-MACHADO, C. de M.; DINIZ, F. de A. dos S.; INOUE-NAGATA, A. K.; ANDRADE, E. C. de; SANTIAGO, T. R. Do cossupressão à tecnologia de RNAí: histórico, mecanismo e exemplos de aplicação tópica de RNA para controle de fitopatógenos. In:RIOS, J. A.; ALMEIDA, L. C.; SOUZA, E. B. de (ed.). Resistência de plantas a patógenos. Recife: Universidade Federal Rural de Pernambuco, 2021.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Hortaliças. |
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| 12. | | SILVA, L. P.; VAZ, G. M. da R.; PUPE, J. M.; CHAFRAN, L. S.; ARAUJO NETO, L. A.; SANTIAGO, T. R.; ARAÚJO, T. F.; POLEZ, V. L. P. Green nanoparticles for biomedical and bioengineering applications. In: SHUKLA, A. (Ed.). Nanoparticles and their biomedical applications. Singapore: Springer, 2020. p. 225-262| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 13. | | SANTIAGO, T. R.; BONATTO, C. C.; ROSSATO, M.; LOPES, C. A. P.; LOPES, C. A.; MIZUBUTI, E. S. G.; SILVA, L. P. Green synthesis of silver nanoparticles using tomato leaf extract and their entrapment in chitosan nanoparticles to control bacterial wilt. Journal of the Science of Food and Agriculture, v. 99, n. 9, p. 4248-4259, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Hortaliças; Embrapa Recursos Genéticos e Biotecnologia. |
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| 14. | | PEREIRA, V. M.; DIAS, B. B. A.; SANTIAGO, T. R.; KOBAYASHI, A. K.; MOLINARI, H. B. C.; FAVARO, L. C. de L. Identification and expression of sugarcane culm-specific expansin genes. In: BRAZILIAN BIOENERGY AND TECNOLOGY CONFERENCE - BBEST, 2017, Campos do Jordão, SP. [Resumos ...]. Campinas: SBE, 2017. Não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 15. | | ROLIM, L.; SANTIAGO, T. R.; REIS JUNIOR, F. B. dos; MENDES, I. de C.; DO VALE, H. M. M.; HUNGRIA, M.; SILVA, L. P. Identification of soybean Bradyrhizobium strains used in commercial inoculants in Brazil by MALDI-TOF mass spectrometry. Brazilian Journal of Microbiology, v. 50, n. 4, p. 905-914, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
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| 16. | | SANTIAGO, T. R.; PEREIRA, V. M.; SOUZA, W. R.; STEINDORFF, A. S.; DIAS, B. B. A.; GASPAR, M.; FAVARO, L. C. de L.; FORMIGHIERI, E. F.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Genome-wide identification, characterization and expression profile analysis of expansins gene family in sugarcane (Saccharum spp.). PLoS One, v. 13, n. 1, artigo e0191081, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroenergia. |
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| 17. | | SILVA, L. P. da; SILVEIRA, A. P.; BONATTO, C. C.; BARBOSA, E. F.; MEDEIROS, K. A.; VIOL, L. C. de S.; PEREIRA, T. M.; SANTIAGO, T. R.; POLEZ, V. L. P.; LAURIA, V. B. M. Sustainable exploitation of agricultural, forestry, and food residues for green nanotechnology applications. In: THANGADURAI, D.; ISLAM, S.; SANGEETHA, J.; MARTINS, N. (Org.). Biogenic nanomaterials: structural properties and functional applications. Palm Bay: Apple Academic, 2021. Cap. 12, p. 301-345. (Innovations in Biotechnology, 1) Na Publicação: Luciano Paulino Silva.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 18. | | SOUZA, W. R. de; PACHECO, T. F.; DUARTE, K. E.; SAMPAIO, B. L.; OLIVEIRA, P. A. de; MARTINS, P. M.; SANTIAGO, T. R.; FORMIGHIERI, E. F.; VINECKY, F.; RIBEIRO, A. P.; DIAS, B. B. A.; KOBAYASHI, A. K.; MITCHELL, R. A. C.; RODRIGUES, D. de S.; MOLINARI, H. B. C. Silencing of a BAHD acyltransferase in sugarcane increases biomass digestibility. Biotechnology for Biofuels, v. 12, n. 111, p. 1-14, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroenergia. |
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| 19. | | RIBEIRO, A. P.; VINECKY, F.; DUARTE, K. E.; SANTIAGO, T. R.; CASARI, R. A. das C. N.; HELL, A. F.; DIAS, B. B. A.; MARTINS, P. K.; CENTENO, D. da C.; OLIVEIRA, P. A. de; CANÇADO, G. M. de A.; MAGALHAES, J. V. de; KOBAYASHI, A. K.; SOUZA, W. R. de; MOLINARI, H. B. C. Enhanced aluminum tolerance in sugarcane: evaluation of SbMATE overexpression and genome-wide identification of ALMTs in Saccharum spp. BMC Plant Biology, v. 21, p. 1-15, 2021. Article number: 300.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Agroenergia; Embrapa Milho e Sorgo. |
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| 20. | | NAKAYAMA, T. J.; RODRIGUES, F. A.; NEUMAIER, N.; GOMES, J. M.; MOLINARI, H. B. C.; SANTIAGO, T. R.; FORMIGHIERI, E. F.; BASSO, M. F.; FARIAS, J. R. B.; EMYGDIO, B. M.; OLIVEIRA, A. C. B. de; CAMPOS, A. D.; BORÉM, A.; HARMON, F. G.; MERTZ-HENNING, L. M.; NEPOMUCENO, A. L. Insights into soybean transcriptome reconfiguration under hypoxic stress: Functional, regulatory, structural, and compositional characterization. PLoS ONE, v. 12, n. 11, e0187920, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 25 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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