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Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
04/06/2024 |
Data da última atualização: |
02/08/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FERREIRA, D. da S.; MARTINS, A. da C. Q.; BERBERT, P. S.; ANJOS, R. M. dos; SARAIVA, M. A. de P.; BRASILEIRO, A. C. M.; MILLER, R. N. G.; GUIMARAES, P. M. |
Afiliação: |
DEZIANY DA SILVA FERREIRA, UNIVERSITY OF BRASILIA; ANDRESSA DA CUNHA QUINTANA MARTINS; PEDRO SOUZA BERBERT; RENAN MIGUEL DOS ANJOS, UNIVERSITY OF BRASILIA; MARIO ALFREDO DE PASSOS SARAIVA, CENARGEN; ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN; ROBERT NEIL GERARD MILLER, UNIVERSITY OF BRASILIA; PATRICIA MESSEMBERG GUIMARAES, CENARGEN. |
Título: |
A wild Arachis Endochitinase enhances Sclerotinia resistance in transgenic plants. |
Ano de publicação: |
2024 |
Fonte/Imprenta: |
Tropical Plant Biology, v. 17, p. 138-155, 2024. |
DOI: |
https://doi.org/10.1007/s12042-024-09359-z |
Idioma: |
Inglês |
Conteúdo: |
Plant endochitinases promote the cleavage of chitin, a polymer naturally found in the cell walls of fungi and insects. Although such enzymes are widely employed in plant genetic engineering to increase tolerance to pathogenic fungi, endochitinases from wild germplasm are poorly exploited for biotechnological purposes. Wild peanut species (Arachis spp.) have evolved under a range of environmental conditions and display distinct defensive adaptations, harboring high levels of genetic diversity and constituting an attractive source of resistance genes against pathogens. Arachis stenosperma shows broad resistance against various biotic stresses such as nematodes, fungi, and viruses. Previous transcriptome and proteomic studies on A. stenosperma challenged with fungi and nematodes identified differentially expressed genes (DEGs) involved in plant defense responses, including an upregulated endochitinase (AsECHI1). Here, we characterized endochitinases from 12 different legumes, including wild Arachis species, and evaluated the effects of overexpression of AsECHI1 for control of Sclerotinia sclerotiorum in tobacco, singly and in association with an expansin-like B defense-priming gene (AdEXLB8). Both singly and pyramided transgenic tobacco lines overexpressing AsECHI1 exhibited a delay in disease progression, and up to a 46% reduction in fungal lesions. Further analysis of transgenic plants showed that the overexpression of AsECHI1 led to an increased expression of defense-related genes in the jasmonic acid, auxin, and ethylene biosynthesis pathways, as well as a substantial accumulation of H2O2. These results suggest that the AsECHI1 gene isolated from wild Arachis has the potential to enhance resistance against this highly damaging necrotrophic fungal pathogen, reducing environmental damage related to the use of fungicides and increasing crop sustainability. MenosPlant endochitinases promote the cleavage of chitin, a polymer naturally found in the cell walls of fungi and insects. Although such enzymes are widely employed in plant genetic engineering to increase tolerance to pathogenic fungi, endochitinases from wild germplasm are poorly exploited for biotechnological purposes. Wild peanut species (Arachis spp.) have evolved under a range of environmental conditions and display distinct defensive adaptations, harboring high levels of genetic diversity and constituting an attractive source of resistance genes against pathogens. Arachis stenosperma shows broad resistance against various biotic stresses such as nematodes, fungi, and viruses. Previous transcriptome and proteomic studies on A. stenosperma challenged with fungi and nematodes identified differentially expressed genes (DEGs) involved in plant defense responses, including an upregulated endochitinase (AsECHI1). Here, we characterized endochitinases from 12 different legumes, including wild Arachis species, and evaluated the effects of overexpression of AsECHI1 for control of Sclerotinia sclerotiorum in tobacco, singly and in association with an expansin-like B defense-priming gene (AdEXLB8). Both singly and pyramided transgenic tobacco lines overexpressing AsECHI1 exhibited a delay in disease progression, and up to a 46% reduction in fungal lesions. Further analysis of transgenic plants showed that the overexpression of AsECHI1 led to an increased expression of defense-related... Mostrar Tudo |
Palavras-Chave: |
A stenosperma; Defense Genes; Necrotrophs; S Sclerotiorum; Stem-rot; White-mold. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02744naa a2200289 a 4500 001 2164663 005 2024-08-02 008 2024 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s12042-024-09359-z$2DOI 100 1 $aFERREIRA, D. da S. 245 $aA wild Arachis Endochitinase enhances Sclerotinia resistance in transgenic plants.$h[electronic resource] 260 $c2024 520 $aPlant endochitinases promote the cleavage of chitin, a polymer naturally found in the cell walls of fungi and insects. Although such enzymes are widely employed in plant genetic engineering to increase tolerance to pathogenic fungi, endochitinases from wild germplasm are poorly exploited for biotechnological purposes. Wild peanut species (Arachis spp.) have evolved under a range of environmental conditions and display distinct defensive adaptations, harboring high levels of genetic diversity and constituting an attractive source of resistance genes against pathogens. Arachis stenosperma shows broad resistance against various biotic stresses such as nematodes, fungi, and viruses. Previous transcriptome and proteomic studies on A. stenosperma challenged with fungi and nematodes identified differentially expressed genes (DEGs) involved in plant defense responses, including an upregulated endochitinase (AsECHI1). Here, we characterized endochitinases from 12 different legumes, including wild Arachis species, and evaluated the effects of overexpression of AsECHI1 for control of Sclerotinia sclerotiorum in tobacco, singly and in association with an expansin-like B defense-priming gene (AdEXLB8). Both singly and pyramided transgenic tobacco lines overexpressing AsECHI1 exhibited a delay in disease progression, and up to a 46% reduction in fungal lesions. Further analysis of transgenic plants showed that the overexpression of AsECHI1 led to an increased expression of defense-related genes in the jasmonic acid, auxin, and ethylene biosynthesis pathways, as well as a substantial accumulation of H2O2. These results suggest that the AsECHI1 gene isolated from wild Arachis has the potential to enhance resistance against this highly damaging necrotrophic fungal pathogen, reducing environmental damage related to the use of fungicides and increasing crop sustainability. 653 $aA stenosperma 653 $aDefense Genes 653 $aNecrotrophs 653 $aS Sclerotiorum 653 $aStem-rot 653 $aWhite-mold 700 1 $aMARTINS, A. da C. Q. 700 1 $aBERBERT, P. S. 700 1 $aANJOS, R. M. dos 700 1 $aSARAIVA, M. A. de P. 700 1 $aBRASILEIRO, A. C. M. 700 1 $aMILLER, R. N. G. 700 1 $aGUIMARAES, P. M. 773 $tTropical Plant Biology$gv. 17, p. 138-155, 2024.
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