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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
21/05/2024 |
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Data da última atualização: |
21/05/2024 |
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Autoria: |
PERRIN, E.; MICLO, L.; DRIOU, A.; LINDEN, G. |
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Afiliação: |
EMMANUEL PERRIN; LAURENT MICLO; ALAIN DRIOU; GUY LINDEN. |
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Título: |
Analysis of peptides by amino acids composition: contribution of ultraviolet derivative spectrophotometry and retention time prediction. |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
Analytical Communications, v. 33, p. 143-147, May, 1996. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Amino acid composition analysis is sometimes used for the identification of peptides obtained from the hydrolysis of a protein of known sequence. Nevertheless, the interpretation of the analysis can be difficult if only one hydrochloric acid hydrolysis can be performed because of the partial destruction of some amino acids and the lack of cleavage of certain peptidic bonds. In this paper we propose combining the analysis of amino acids by two techniques (derivative UV spectrometry and retention time estimation) associated on-line with the purification step of the peptides (reversed-phase HPLC). A set of 56 peptides from the peptic and chymotryptic hydrolysis of bovine αs1-casein (αs1-CN) were analysed in this manner. The difference between the theoretical and observed retention times was 1.9 min, for aromatic amino acids ratios the difference was 4.0%. The ambiguities coming from repeated residues in the sequence or the presence of Trp residues, destroyed by acidic hydrolysis, were solved and a fragment of the αs1-CN sequence could be attributed to each peptide. |
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Palavras-Chave: |
Chymotryptic hydrolysis. |
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Thesaurus Nal: |
AlphaS1-casein; Amino acids; Peptides. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01730naa a2200205 a 4500
001 2164382
005 2024-05-21
008 1996 bl uuuu u00u1 u #d
100 1 $aPERRIN, E.
245 $aAnalysis of peptides by amino acids composition$bcontribution of ultraviolet derivative spectrophotometry and retention time prediction.$h[electronic resource]
260 $c1996
520 $aAbstract: Amino acid composition analysis is sometimes used for the identification of peptides obtained from the hydrolysis of a protein of known sequence. Nevertheless, the interpretation of the analysis can be difficult if only one hydrochloric acid hydrolysis can be performed because of the partial destruction of some amino acids and the lack of cleavage of certain peptidic bonds. In this paper we propose combining the analysis of amino acids by two techniques (derivative UV spectrometry and retention time estimation) associated on-line with the purification step of the peptides (reversed-phase HPLC). A set of 56 peptides from the peptic and chymotryptic hydrolysis of bovine αs1-casein (αs1-CN) were analysed in this manner. The difference between the theoretical and observed retention times was 1.9 min, for aromatic amino acids ratios the difference was 4.0%. The ambiguities coming from repeated residues in the sequence or the presence of Trp residues, destroyed by acidic hydrolysis, were solved and a fragment of the αs1-CN sequence could be attributed to each peptide.
650 $aAlphaS1-casein
650 $aAmino acids
650 $aPeptides
653 $aChymotryptic hydrolysis
700 1 $aMICLO, L.
700 1 $aDRIOU, A.
700 1 $aLINDEN, G.
773 $tAnalytical Communications$gv. 33, p. 143-147, May, 1996.
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