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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
10/04/2024 |
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Data da última atualização: |
15/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RIBEIRO, A. C. S.; PINTO, P. H. N.; SANTOS, J. D. R.; RAMALHO, N. P.; LAEBER, C. C. R.; BALARO, M. F. A.; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da; UNGERFELD, R.; BRANDÃO, F. Z. |
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Afiliação: |
ANA CLARA SARZEDAS RIBEIRO, UNIVERSIDADE FEDERAL FLUMINENSE; PEDRO HENRIQUE NICOLAU PINTO, UNIVERSIDADE FEDERAL FLUMINENSE; JULIANA DANTAS RODRIGUES SANTOS, UNIVERSIDADE FEDERAL FLUMINENSE; NADIALA PORTO RAMALHO, UNIVERSIDADE FEDERAL FLUMINENSE; CAMILA CORREA ROZA LAEBER, UNIVERSIDADE FEDERAL FLUMINENSE; MARIO FELIPE ALVAREZ BALARO, UNIVERSIDADE FEDERAL FLUMINENSE; RIBRIO IVAN TAVARES PEREIRA BATISTA, UNIVERSIDADE FEDERAL FLUMINENSE; JOANNA MARIA GONÇALVES SOUZA-FABJAN, UNIVERSIDADE FEDERAL FLUMINENSE; JEFERSON FERREIRA DA FONSECA, CNPC; RODOLFO UNGERFELD, UNIVERSIDAD DE LA REPÚBLICA; FELIPE ZANDONADI BRANDÃO, UNIVERSIDADE FEDERAL FLUMINENSE. |
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Título: |
The pFSH dose affects the efficiency of in vivo embryo production in Santa Inês ewes. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Animal Reproduction Science, v. 64, 107459, May, 2024. |
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DOI: |
https://doi.org/10.1016/j.anireprosci.2024.107459 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: This study compared the follicular growth, superovulatory response, and in vivo embryo production after administering two doses of porcine follicle-stimulating hormone (pFSH) in Santa Inês ewes. The estrous cycle of 36 multiparous ewes was synchronized with the Day 0 protocol and superovulated with 133 mg (G133, n=18) or 200 mg (G200, n=18) of pFSH. Ultrasonographic evaluations of the ovaries were performed, ewes were mated and submitted to non-surgical embryo recovery. Viable blastocysts were stained with Nile Red and Hoechst. The G200 had a greater number of medium and large follicles, as well as a larger size of the third largest follicle. A total of 97.2% (35/36) of the ewes came into estrus and it was possible to transpose cervix in 80.6% (29/36). There were no effects of treatments in the response to superovulation, the proportion of ewes in which was possible to transpose the cervix, the number of corpora lutea, the number of anovulatory follicles, the proportion of ewes flushed with at least one recovered structure, number of recovered structures, number of viable embryos, viability rate, and recovery rate. The G200 ewes were in estrus for a longer period of time than the G133 ewes (54.0 ± 4.5 h vs. 40.3 ± 3.6 h) and produced more freezable embryos (6.5 ± 1.6 vs. 2.3 ± 0.7) than G133. Both doses promoted an efficient superovulatory response and did not affect embryonic lipid accumulation. The dose of 200 mg of pFSH showed greater potential to increase the superovulatory response, as it increased follicular recruitment and the recovery of freezable embryos. MenosAbstract: This study compared the follicular growth, superovulatory response, and in vivo embryo production after administering two doses of porcine follicle-stimulating hormone (pFSH) in Santa Inês ewes. The estrous cycle of 36 multiparous ewes was synchronized with the Day 0 protocol and superovulated with 133 mg (G133, n=18) or 200 mg (G200, n=18) of pFSH. Ultrasonographic evaluations of the ovaries were performed, ewes were mated and submitted to non-surgical embryo recovery. Viable blastocysts were stained with Nile Red and Hoechst. The G200 had a greater number of medium and large follicles, as well as a larger size of the third largest follicle. A total of 97.2% (35/36) of the ewes came into estrus and it was possible to transpose cervix in 80.6% (29/36). There were no effects of treatments in the response to superovulation, the proportion of ewes in which was possible to transpose the cervix, the number of corpora lutea, the number of anovulatory follicles, the proportion of ewes flushed with at least one recovered structure, number of recovered structures, number of viable embryos, viability rate, and recovery rate. The G200 ewes were in estrus for a longer period of time than the G133 ewes (54.0 ± 4.5 h vs. 40.3 ± 3.6 h) and produced more freezable embryos (6.5 ± 1.6 vs. 2.3 ± 0.7) than G133. Both doses promoted an efficient superovulatory response and did not affect embryonic lipid accumulation. The dose of 200 mg of pFSH showed greater potential to increase the s... Mostrar Tudo |
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Palavras-Chave: |
Follicular growth; Lipid quantification; MOET; Multiple ovulation. |
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Thesaurus Nal: |
Breeding and Genetic Improvement; Embryo transfer; Follicle-stimulating hormone; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| 3. |  | SILVA, E. B. da. Dendeicultura no Brasil; diagnóstico. Manaus: EMBRAPA-CPAA, 1993. 18 p. Trabalho preparado para a X Conferência Internacional de Palma Aceiteira, BUROTROP/IICA/FEDEPALMA, Santa Marta/Colômbia - 24-29, maio 93.| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 14. | | SILVA, E. B. da; AMBLARD, P.; BERTHAUD, J.; SEGUIN, M. Genetic diversity and relationship in American and African oil palm as revealed by RFLP and AFLP molecular markers. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 37, n. 8, p. 1105-1114, ago. 2002 Título em português: Diversidade e relação genética entre o dendê americano e o africano revelada por marcadores moleculares do tipo RFLP e AFLP.| Biblioteca(s): Embrapa Unidades Centrais. |
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