| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Gado de Leite. |
|
Data corrente: |
30/01/2024 |
|
Data da última atualização: |
30/01/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
REIS, C. B. dos; OTENIO, M. H.; MELO JÚNIOR, A. M. de; DORNELAS, J. C. M.; CARMO, P. H. F. do; VIANA, R. de O.; RICOY, A. C. S.; ALVES, V. de S. |
|
Afiliação: |
CAMILA BRANDA DOS REIS, UNIVERSIDADE FEDERAL DE MINAS GERAIS; MARCELO HENRIQUE OTENIO, CNPGL; ANDERSON MACHADO DE MELO JUNIOR, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; JOAO CARLOS MAIA DORNELAS, UNIVERSIDADE FEDERAL DE MINAS GERAIS; PAULO HENRIQUE FONSECA DO CARMO, UNIVERSIDADE FEDERAL DE MINAS GERAIS; ROBERTA DE OLIVEIRA VIANA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; ANA CAROLINA SANTOS RICOY, UNIVERSIDADE FEDERAL DE MINAS GERAIS; VIVIANE DE SOUZA ALVES, UNIVERSIDADE FEDERAL DE MINAS GERAIS. |
|
Título: |
Virulence profile of Candida spp. isolated from an anaerobic biodigester supplied with dairy cattle waste. |
|
Ano de publicação: |
2024 |
|
Fonte/Imprenta: |
Microbial Pathogenesis, v. 187, 106516, 2024. |
|
DOI: |
https://doi.org/10.1016/j.micpath.2023.106516 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Anaerobic biodigesters play a crucial role in enhancing animal waste management. However, the presence of pathogens in the biodigestion process poses a significant concern. Candida spp., a widespread fungus known for its opportunistic nature and adaptability to diverse environmental conditions, including reciprocal transmission between humans and animals, is one such pathogen of concern. Therefore, it is imperative to assess the virulence profile of Candida spp. originating from anaerobic biodigestion processes. Here we demonstrate that strains isolated from the biodigestion process of dairy cattle waste exhibit noteworthy virulence mechanisms, surpassing the virulence of clinical control strains. After we identified strains from affluent, effluent, and biofertilizer, we observed that all analyzed isolates produced biofilm. Additionally, a substantial proportion of these isolates demonstrated phospholipase production, while only a few strains exhibited protease production. Furthermore, all strains exhibited resistance or dose-dependent responses to amphotericin B and itraconazole, with the majority displaying resistance to fluconazole. In the in vivo test, we observed a significant correlation (p < 0.05) between the LT50 and biofilm formation as well as hyphae/pseudohyphae production. Additionally, some isolates demonstrated a quicker nematode-killing capacity compared to clinical controls. Our findings underscore the considerable pathogenic potential of certain Candida species present in the dynamics of anaerobic biodigestion. Importantly, the anaerobic biodigester system did not eliminate Candida strains from dairy cattle waste, highlighting the need for caution in utilizing biodigester products. We advocate for further studies to explore the virulence of other microorganisms in various animal production contexts. Furthermore, our results emphasize the urgency of enhancing waste treatment methods to effectively eliminate pathogens and curb their potential dissemination. MenosAnaerobic biodigesters play a crucial role in enhancing animal waste management. However, the presence of pathogens in the biodigestion process poses a significant concern. Candida spp., a widespread fungus known for its opportunistic nature and adaptability to diverse environmental conditions, including reciprocal transmission between humans and animals, is one such pathogen of concern. Therefore, it is imperative to assess the virulence profile of Candida spp. originating from anaerobic biodigestion processes. Here we demonstrate that strains isolated from the biodigestion process of dairy cattle waste exhibit noteworthy virulence mechanisms, surpassing the virulence of clinical control strains. After we identified strains from affluent, effluent, and biofertilizer, we observed that all analyzed isolates produced biofilm. Additionally, a substantial proportion of these isolates demonstrated phospholipase production, while only a few strains exhibited protease production. Furthermore, all strains exhibited resistance or dose-dependent responses to amphotericin B and itraconazole, with the majority displaying resistance to fluconazole. In the in vivo test, we observed a significant correlation (p < 0.05) between the LT50 and biofilm formation as well as hyphae/pseudohyphae production. Additionally, some isolates demonstrated a quicker nematode-killing capacity compared to clinical controls. Our findings underscore the considerable pathogenic potential of certain Candida spec... Mostrar Tudo |
|
Palavras-Chave: |
Antifúngico; Biodigestão; Levedura; Resíduo animal. |
|
Thesagro: |
Biodigestor; Fungo; Gado Leiteiro; Resíduo. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
null
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 7 | |
| 1. |  | MORALES, A.; O'ROURKE, J. A.; BORÉM, A.; ABDELNOOR, R. V.; PEDLEY, K. F.; WHITHAM, S. A.; GRAHAM, M. A. Combining transcriptome analyses and virus induced gene silencing to identify genes in the Rpp4-mediated asian soybean rust resistance Pathway. In: MOLECULAR & CELLULAR BIOLOGY OF TE SOYBEAN, 14., Des Moines, 2012. [Proceedings...]. Des Moines: Iowa State University, 2012. p. 137.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
|    |
| 2. | | FARMAN, M.; PETERSON, G.; CHEN, L.; STARNES, J.; VALENT, B.; BACHI, P.; MURDOCK, L.; HERSHMAN, D.; PEDLEY, K.; FERNANDES, J. M. C.; BAVARESCO, J. The Lolium pathotype of Magnaporthe oryzae recovered from a single blasted wheat plant in the United States. Plant Disease, St. Paul, v. 101, n. 5, p. 684-692, May 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Trigo. |
| |
| 4. | | GLADIEUX, P.; CONDON, B.; RAVEL, S.; SOANES, D.; MACIEL, J. L. N.; NHANI JUNIOR, A.; CHEN, L.; TERAUCHI, R.; LEBRUN, M.-H.; THARREAU, D.; MITCHELL, T.; PEDLEY, K. F.; VALENT, B.; TALBOT, N. J.; FARMAN, M.; FOURNIER, E. Gene flow between divergent cereal- and grass-specific lineages of the rice blast fungus Magnaporthe oryzae. mBio, v. 9, n. 1, e01219-17, Jan./Feb. 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Trigo. |
| |
| 5. | | MEYER, J. D. F. SILVA, D. C. G. MORTEL, M. van de; PEDLEY, K. F. HILL, J. H. SHOEMAKER, R. C. ABDELNOOR, R. V. WHITHAM, S. A. GRAHAM, M. A. Identification and analyses of candidate Genes for Rpp4 mediated resistance to Asian Soybean rust in soybean (Glycine max). In: THE INTERNATIONAL CONFERENCE ON THE STATUS OF PLANT & ANIMAL GENOME RESEARCH, 17., 2009, San Diego. Final Abstract Guide. [S.l.], 2009. Guide. [S.l.], 2009. p. 47, W474.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
|  |
| 6. | | MEYER, J. D. F.; SILVA, D. C. G.; YANG, C.; PEDLEY, K. F.; ZHANG, C.; MORTEL, M. van de; HILL, J. H.; SHOEMAKER, R. C.; ABDELNOOR, R. V.; WHITHAM, S. A.; GRAHAM, M. A. Identification and analyses of candidate genes for Rpp4-Mediated resistance to Asian Soybean Rust in Soybean. Plant Physiology, Bethesda, v. 150, n. 1, p. 295-307, may 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
|  |
| 7. | | MORALES, A. M. A. P.; O'ROURKE, J. A.; MORTEL, M. van de; SCHEIDER, K. T.; BANCROFT, T. J.; BORÉM, A.; NELSON, R. T.; NETTLETON, D.; BAUM, T. J.; SHOEMAKER, R. C.; FREDERICK, R. D.; ABDELNOOR, R. V.; PEDLEY, K. F.; WHITHAM, S. A.; GRAHAM, M. A. Transcriptome analyses and virus induced gene silencing identify genes in the Rpp4-mediated Asian soybean rust resistance pathway. Functional Plant Biology, v. 40, n. 10, p. 1029-1047, sept. 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
| |
| Registros recuperados : 7 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
