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Registro Completo |
Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
Data corrente: |
13/12/2023 |
Data da última atualização: |
13/12/2023 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
ALVES, T.; GLEISSLITZ, S. M.; FERREIRA, R. M.; SCHERF, K. A.; TAKEITI, C. Y.; FERREIRA, M. S. L. |
Afiliação: |
THAIS ALVES, UNIRIO; SABRINA MARIA GLEISSLITZ, Karlsruhe Institute of Technology; RENATA MERENDA FERREIRA, UNIRIO; KATHARINA ANNE SCHERF; CRISTINA YOSHIE TAKEITI, CTAA; MARIANA SIMÕES LARRAZ FERREIRA, UNIRIO. |
Título: |
Proteomic insights into bread-making: fermentation effects on wheat protein profiles. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
In: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DE ALIMENTOS E NUTRIÇÃO, 15., 2023, Campinas. A revolução da ciência de alimentos e nutrição: alimentando o mundo de forma sustentável: caderno [eletrônico] de resumos. Campinas: Galoá, 2023. |
Idioma: |
Inglês |
Notas: |
Pôster 167620; SLACAN. |
Conteúdo: |
Common wheat (T. aestivum) dominates cultivated wheat at around 95%. Gluten proteins confer distinct rheological properties, are the main responsible for viscoelasticity, facilitate dough leavening and structural maintenance in breadmaking. Advanced proteomic techniques like UHPLC-MS/MS aid in deciphering protein contributions to end product quality and identifying optimal wheat cultivars for baking. This research explored how S. cerevisiae fermentation affects protein profiles in breads from two wheat flours: ORS Agile (bread/improver wheat) and ORS Vintecinco (biscuit wheat). Proteins were categorized by solubility (AG, Gli, reduced Glu) and assessed using RP-HPLC and Kjeldahl methods. Proteomic data was obtained by NanoLC-MS/MS analysis on a Q Exactive Plus Orbitrap, processed via MaxQuant using UniprotKB Triticum database. Total protein ranged from 8.2% to 12.9%. ORS Agile had lower extractability (62%) than ORS Vintecinco (98%). Both genotypes showed reduced AG and Gli, and increased Glu levels in breads. Glu increase was more significant in ORS Vintecinco (~200%) than ORS Agile (~54%). Impact during breadmaking was notable for α-gliadin and γ-gliadin, with reduced expression intensities after kneading, dough formation and fermentation. LMW-GS contents increased after processing, while HMW-glutenins exhibited variable behavior depending on the analyzed genotype. Considering proteomic analysis, 250 proteins were evaluated (187 for ORS Agile, 122 for ORS Vintecinco, 59 common). Distinctive proteins could serve as potential biomarkers for flour composition and bread origin. Proteomic analysis unveiled composition differences among products from contrasting flour samples. Discrepancies in structure and composition of gluten proteins between genotypes could influence the susceptibility to modifications induced by processing. ORS Vintecinco was notably more affected by processing when compared to ORS Agile. The hypothesis to explain this finding is that weak wheat flour may be more inclined to suffer changes in gluten content during processing due to its lower gluten content when compared to strong wheat flour, facilitating protein polymerization. MenosCommon wheat (T. aestivum) dominates cultivated wheat at around 95%. Gluten proteins confer distinct rheological properties, are the main responsible for viscoelasticity, facilitate dough leavening and structural maintenance in breadmaking. Advanced proteomic techniques like UHPLC-MS/MS aid in deciphering protein contributions to end product quality and identifying optimal wheat cultivars for baking. This research explored how S. cerevisiae fermentation affects protein profiles in breads from two wheat flours: ORS Agile (bread/improver wheat) and ORS Vintecinco (biscuit wheat). Proteins were categorized by solubility (AG, Gli, reduced Glu) and assessed using RP-HPLC and Kjeldahl methods. Proteomic data was obtained by NanoLC-MS/MS analysis on a Q Exactive Plus Orbitrap, processed via MaxQuant using UniprotKB Triticum database. Total protein ranged from 8.2% to 12.9%. ORS Agile had lower extractability (62%) than ORS Vintecinco (98%). Both genotypes showed reduced AG and Gli, and increased Glu levels in breads. Glu increase was more significant in ORS Vintecinco (~200%) than ORS Agile (~54%). Impact during breadmaking was notable for α-gliadin and γ-gliadin, with reduced expression intensities after kneading, dough formation and fermentation. LMW-GS contents increased after processing, while HMW-glutenins exhibited variable behavior depending on the analyzed genotype. Considering proteomic analysis, 250 proteins were evaluated (187 for ORS Agile, 122 for ORS Vintecinco, 59 co... Mostrar Tudo |
Palavras-Chave: |
Bakery; Foodomics; Wheat quality. |
Categoria do assunto: |
Q Alimentos e Nutrição Humana |
Marc: |
LEADER 03017nam a2200217 a 4500 001 2159644 005 2023-12-13 008 2023 bl uuuu u00u1 u #d 100 1 $aALVES, T. 245 $aProteomic insights into bread-making$bfermentation effects on wheat protein profiles.$h[electronic resource] 260 $aIn: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DE ALIMENTOS E NUTRIÇÃO, 15., 2023, Campinas. A revolução da ciência de alimentos e nutrição: alimentando o mundo de forma sustentável: caderno [eletrônico] de resumos. Campinas: Galoá$c2023 500 $aPôster 167620; SLACAN. 520 $aCommon wheat (T. aestivum) dominates cultivated wheat at around 95%. Gluten proteins confer distinct rheological properties, are the main responsible for viscoelasticity, facilitate dough leavening and structural maintenance in breadmaking. Advanced proteomic techniques like UHPLC-MS/MS aid in deciphering protein contributions to end product quality and identifying optimal wheat cultivars for baking. This research explored how S. cerevisiae fermentation affects protein profiles in breads from two wheat flours: ORS Agile (bread/improver wheat) and ORS Vintecinco (biscuit wheat). Proteins were categorized by solubility (AG, Gli, reduced Glu) and assessed using RP-HPLC and Kjeldahl methods. Proteomic data was obtained by NanoLC-MS/MS analysis on a Q Exactive Plus Orbitrap, processed via MaxQuant using UniprotKB Triticum database. Total protein ranged from 8.2% to 12.9%. ORS Agile had lower extractability (62%) than ORS Vintecinco (98%). Both genotypes showed reduced AG and Gli, and increased Glu levels in breads. Glu increase was more significant in ORS Vintecinco (~200%) than ORS Agile (~54%). Impact during breadmaking was notable for α-gliadin and γ-gliadin, with reduced expression intensities after kneading, dough formation and fermentation. LMW-GS contents increased after processing, while HMW-glutenins exhibited variable behavior depending on the analyzed genotype. Considering proteomic analysis, 250 proteins were evaluated (187 for ORS Agile, 122 for ORS Vintecinco, 59 common). Distinctive proteins could serve as potential biomarkers for flour composition and bread origin. Proteomic analysis unveiled composition differences among products from contrasting flour samples. Discrepancies in structure and composition of gluten proteins between genotypes could influence the susceptibility to modifications induced by processing. ORS Vintecinco was notably more affected by processing when compared to ORS Agile. The hypothesis to explain this finding is that weak wheat flour may be more inclined to suffer changes in gluten content during processing due to its lower gluten content when compared to strong wheat flour, facilitating protein polymerization. 653 $aBakery 653 $aFoodomics 653 $aWheat quality 700 1 $aGLEISSLITZ, S. M. 700 1 $aFERREIRA, R. M. 700 1 $aSCHERF, K. A. 700 1 $aTAKEITI, C. Y. 700 1 $aFERREIRA, M. S. L.
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Registro original: |
Embrapa Agroindústria de Alimentos (CTAA) |
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201. | | COELHO, G. T. C. P.; REZENDE, R. K. S.; TORGA, P. P.; PETRILLO, C. P.; CARNEIRO, A. A.; CARNEIRO, N. P.; PINHO, R. G. V.; PAIVA, R. Embriogênese somática de linhagens tropicais de milho (Zea mays) em diferentes concentrações de 2,4-D. Horticultura Brasileira, v. 23, n. 2, p. 600, ago. 2005. Suplemento. Edição dos resumos do 45º Congresso Brasileiro de Olericultura; 15º Congresso Brasileiro de Floricultura e Plantas Ornamentais; 2º Congresso Brasileiro de Cultura de Tecidos de Plantas realizado em Fortaleza.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Milho e Sorgo. |
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203. | | SENA, M. R.; GUIMARAES, P. E. de O.; SOUZA, J. C.; COSTA, L. P.; SCHAFFERT, R. E.; RIBEIRO, P. E. de A.; PACHECO, C. A. P. Estabilidade dos teores de minerais em grãos de variedades de milho. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 4., 2007, São Lourenço. Melhoramento de plantas e agronegócio: Anais... Lavras: UFLA: SBMP, 2007. 1 CD-ROM.Tipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Embrapa Milho e Sorgo. |
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204. | | GOMES, E. A.; OLIVEIRA, C.; CARNEIRO, N. P.; MARRIEL, I. E.; SA, N.; SCOTTO, M.; SCHAFFERT, R. E.; GUIMARAES, C.; ALVES, V. M. C. The effect of Zea mays genotypes contrasting for phosphorus efficiency on mycorrhizal diversity analyzed by denaturing gradient gel electrophoresis (DGGE) and DNA cloning/sequencing. In: INTERNATIONAL CONFERENCE RHIZOSPHERE , 2., 2007, Montpellier. Book of abstracts... Montpellier: INRA, 2007.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Milho e Sorgo. |
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206. | | HOEKENGA, O. A.; BUCKLER, E.; MARON, L.; MAGALHAES, J. V. de; KIRST, M.; KRILL, A.; LYI, S. M.; ROSE, J.; THANNHAUSER, T.; KOCHIAN, L. Joint linkage-association analysis of aluminum tolerance in maize. In: INTERNATIONAL PLANT & ANIMAL GENOMES CONFERENCE, 15., 2007, San Diego, CA. [Proceedings...]. [S. l.: s.n.], 2007.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Milho e Sorgo. |
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207. | | LIGEYO, D. O.; GUDU, S.; OBURA, P.; OKALEBO, J. R.; OTHIENO, C. O.; OMBAKHO, G. A.; SCHAFFERT, R. E.; ALVES, V. M. C.; MAGALHAES, J. V. de; GAMA, E. E. G. e. Improvement of Kenyan maize germplasm for adaptability to low soil pH. In: GRANTEE CONFERENCE FROM PARTNERSHIPS TO COMMUNITY: THE ARC OF CHANGE IN THE CCRP, 2004, Vaals, The Netherlands. [Proceedings]. [Minneapolis]: The McKnight Foundation, 2004.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Milho e Sorgo. |
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209. | | CHIESA, A. C. M.; SISMEIRO, M. N. S.; BROCCO, L. A. F.; EDUARDO, J. A.; HOSHINO, A. T.; SARTORI, L. C.; PASINI, A.; ROGGIA, S. Injúria de Spodoptera frugiperda em milho Bt em sucessão a soja transgênica e submetido a diferentes manejos fitossanitários. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 25., 2014, Goiânia. Entomologia integrada à sociedade para o desenvolvimento sustentável: anais. [Londrina]: SEB, 2014.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
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211. | | ORSO, L.; MACHADO, J. R. de A.; GEHLEN, A.; GAUZE, J. Interação genótipo x local em híbridos de milho. In: MOSTRA DE INICIAÇÃO CIENTÍFICA, 13.; MOSTRA DE PÓS-GRADUAÇÃO DA EMBRAPA TRIGO, 10., 2018, Passo Fundo. Resumos... Brasília, DF: Embrapa, 2018. p. 32. Graduação.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Trigo. |
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218. | | RIOS, S. A.; CARDOSO, W. S.; PAES, M. C. D.; BORÉM, A.; GUIMARAES, P. E. de O.; SCHAFFERT, R. E.; GOMES, A. F. Influência da cor no perfil de carotenóides de grãos de milho para geração de cultivares biofortificados. In: REUNIÃO ANUAL DE BIOFORTIFICAÇÃO NO BRASIL, 2., 2007, Niterói. posteres. Rio de Janeiro: Embrapa Agroindustria de Alimentos, 2007.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Milho e Sorgo. |
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219. | | RIOS, S. A.; CARDOSO, W. S.; PAES, M. C. D.; BOREM, A.; GUIMARAES, P. E. de O.; SCHAFFERT, R. E.; TEIXEIRA, F. F.; GOMES, A. F. Influência da cor no perfil de carotenóides de grãos de milho para geração de cultivares biofortificados. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 4., 2007, São Lourenço. Melhoramento de plantas e agronegócio: Anais...Lavras: UFLA: SBMP, 2007. 1 CD-ROM.Tipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Embrapa Milho e Sorgo. |
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220. | | OLIVEIRA JUNIOR, G. I. de; MORAES, V. B. de; NAKAJIMA, V. M.; SILVA, C. O. da; MORAES, E. A.; PAES, M. C. D.; COSTA, N. M. B. Influência da fibra de pericarpo de milho na biodisponibilidade de ferro em ratos. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DOS ALIMENTOS, 21.; SEMINÁRIO LATINOAMERICANO E DO CARIBE DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 15., 2008, Belo Horizonte. Anais... Belo Horizonte: Sociedade Brasileira de Ciência e Tecnologia de Alimentos, 2008. 1 CD-ROM.Tipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Embrapa Milho e Sorgo. |
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