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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
01/09/2023 |
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Data da última atualização: |
01/09/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MASCARIN, G. M.; KOBORI, N. N.; COLEMAN, J. J.; JACKSON, M. A. |
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Afiliação: |
GABRIEL MOURA MASCARIN, CNPMA; NILCE NAOMI KOBORI, UNITED STATES DEPARTMENT OF AGRICULTURE; JEFFREY J. COLEMAN, AUBURN UNIVERSITY; MARK ALAN JACKSON, UNITED STATES DEPARTMENT OF AGRICULTURE. |
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Título: |
Impact of osmotic stress on production, morphology, and fitness of Beauveria bassiana blastospores. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Applied Microbiology and Biotechnology, v. 107, n. 15, p. 4815-4831, 2023. |
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ISSN: |
0175-7598 |
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DOI: |
http://dx.doi.org/10.1007/s00253-023-12631-z |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Beauveria bassiana is a cosmopolitan entomopathogenic fungus that can infect over 1000 insect species. During growth inside the host, B. bassiana transitions from hyphal to yeast-like unicellular growth as blastospores. Blastospores are well suited as an active ingredient in biopesticides due to their ease of production by liquid fermentation. Herein, we investigated the impact of hyperosmotic growth environments mediated by ionic and non-ionic osmolytes on two strains of B. bassiana (ESALQ1432 and GHA) relevant to growth morphology, blastospore production, desiccation tolerance, and insecticidal activity. Polyethylene glycol (PEG200) increased osmotic pressure in submerged cultures leading to decreased blastospore size but higher blastospore yields for one strain. Morphologically, decreased blastospore size was linked to increased osmotic pressure. However, smaller blastospores from PEG200 supplemented cultures after air-drying exhibited delayed germination. Ionic osmolytes (NaCl and KCl) generated the same osmotic pressure (2.5-2.7 MPa) as 20% glucose and boosted blastospore yields (> 2.0 × 109 blastospores mL-1). Fermentation performed in a bench-scale bioreactor consistently promoted high blastospore yields when using NaCl (2.5 MPa) amended media within 3 days. Mealworm larvae (Tenebrio molitor) were similarly susceptible to NaCl-grown blastospores and aerial conidia in a dose-time-dependent manner. Collectively, these results demonstrate the use of hyperosmotic liquid culture media in triggering enhanced yeast-like growth by B. bassiana. Understanding the role of osmotic pressure on blastospore formation and fitness will hasten the development of viable commercial fungal biopesticides. KEY POINTS: Osmotic pressure plays a critical role in submerged fermentation of B. bassiana. Ionic/non-ionic osmolytes greatly impact blastospore morphology, fitness, and yield. Desiccation tolerance and bioefficacy of blastospores are affected by the osmolyte. MenosAbstract: Beauveria bassiana is a cosmopolitan entomopathogenic fungus that can infect over 1000 insect species. During growth inside the host, B. bassiana transitions from hyphal to yeast-like unicellular growth as blastospores. Blastospores are well suited as an active ingredient in biopesticides due to their ease of production by liquid fermentation. Herein, we investigated the impact of hyperosmotic growth environments mediated by ionic and non-ionic osmolytes on two strains of B. bassiana (ESALQ1432 and GHA) relevant to growth morphology, blastospore production, desiccation tolerance, and insecticidal activity. Polyethylene glycol (PEG200) increased osmotic pressure in submerged cultures leading to decreased blastospore size but higher blastospore yields for one strain. Morphologically, decreased blastospore size was linked to increased osmotic pressure. However, smaller blastospores from PEG200 supplemented cultures after air-drying exhibited delayed germination. Ionic osmolytes (NaCl and KCl) generated the same osmotic pressure (2.5-2.7 MPa) as 20% glucose and boosted blastospore yields (> 2.0 × 109 blastospores mL-1). Fermentation performed in a bench-scale bioreactor consistently promoted high blastospore yields when using NaCl (2.5 MPa) amended media within 3 days. Mealworm larvae (Tenebrio molitor) were similarly susceptible to NaCl-grown blastospores and aerial conidia in a dose-time-dependent manner. Collectively, these results demonstrate the use of hyperosmoti... Mostrar Tudo |
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Palavras-Chave: |
Desiccation tolerance; Osmoadaptation. |
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Thesagro: |
Beauveria Bassiana; Fermentação; Fungo Entomógeno; Fungo Para Controle Biológico; Produção. |
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Thesaurus Nal: |
Biological control agents; Bioreactors; Entomopathogenic fungi; Liquid state fermentation; Virulence. |
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Categoria do assunto: |
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Marc: |
LEADER 03019naa a2200325 a 4500
001 2156317
005 2023-09-01
008 2023 bl uuuu u00u1 u #d
022 $a0175-7598
024 7 $ahttp://dx.doi.org/10.1007/s00253-023-12631-z$2DOI
100 1 $aMASCARIN, G. M.
245 $aImpact of osmotic stress on production, morphology, and fitness of Beauveria bassiana blastospores.$h[electronic resource]
260 $c2023
520 $aAbstract: Beauveria bassiana is a cosmopolitan entomopathogenic fungus that can infect over 1000 insect species. During growth inside the host, B. bassiana transitions from hyphal to yeast-like unicellular growth as blastospores. Blastospores are well suited as an active ingredient in biopesticides due to their ease of production by liquid fermentation. Herein, we investigated the impact of hyperosmotic growth environments mediated by ionic and non-ionic osmolytes on two strains of B. bassiana (ESALQ1432 and GHA) relevant to growth morphology, blastospore production, desiccation tolerance, and insecticidal activity. Polyethylene glycol (PEG200) increased osmotic pressure in submerged cultures leading to decreased blastospore size but higher blastospore yields for one strain. Morphologically, decreased blastospore size was linked to increased osmotic pressure. However, smaller blastospores from PEG200 supplemented cultures after air-drying exhibited delayed germination. Ionic osmolytes (NaCl and KCl) generated the same osmotic pressure (2.5-2.7 MPa) as 20% glucose and boosted blastospore yields (> 2.0 × 109 blastospores mL-1). Fermentation performed in a bench-scale bioreactor consistently promoted high blastospore yields when using NaCl (2.5 MPa) amended media within 3 days. Mealworm larvae (Tenebrio molitor) were similarly susceptible to NaCl-grown blastospores and aerial conidia in a dose-time-dependent manner. Collectively, these results demonstrate the use of hyperosmotic liquid culture media in triggering enhanced yeast-like growth by B. bassiana. Understanding the role of osmotic pressure on blastospore formation and fitness will hasten the development of viable commercial fungal biopesticides. KEY POINTS: Osmotic pressure plays a critical role in submerged fermentation of B. bassiana. Ionic/non-ionic osmolytes greatly impact blastospore morphology, fitness, and yield. Desiccation tolerance and bioefficacy of blastospores are affected by the osmolyte.
650 $aBiological control agents
650 $aBioreactors
650 $aEntomopathogenic fungi
650 $aLiquid state fermentation
650 $aVirulence
650 $aBeauveria Bassiana
650 $aFermentação
650 $aFungo Entomógeno
650 $aFungo Para Controle Biológico
650 $aProdução
653 $aDesiccation tolerance
653 $aOsmoadaptation
700 1 $aKOBORI, N. N.
700 1 $aCOLEMAN, J. J.
700 1 $aJACKSON, M. A.
773 $tApplied Microbiology and Biotechnology$gv. 107, n. 15, p. 4815-4831, 2023.
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