| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Instrumentação. |
|
Data corrente: |
18/04/2023 |
|
Data da última atualização: |
18/04/2023 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
LIMA, N. S. M.; GOMES-PEPE, E. S.; KOCK, F. V. C.; COLNAGO, L. A.; LEMOS, E. G. M. |
|
Afiliação: |
LUIZ ALBERTO COLNAGO, CNPDIA. |
|
Título: |
Dynamics of the role of LacMeta laccase in the complete degradation and detoxification of malachite green. |
|
Ano de publicação: |
2023 |
|
Fonte/Imprenta: |
World Journal of Microbiology and Biotechnology, v. 39, 127, 2023. |
|
Páginas: |
1 - 13 |
|
DOI: |
https://doi.org/10.1007/s11274-023-03572-w |
|
Idioma: |
Inglês |
|
Conteúdo: |
Laccases highlight for xenobiotic bioremediation, as well as application in the fine chemical, textile, biofuel and food industries. In a previous work, we described the preliminary characterization of laccase LacMeta, a promising enzyme for the bioremediation of dyes, able to decolorization malachite green (MG), trypan blue, methylene blue. Here we demonstrate that LacMeta is indeed suitable for the complete degradation and detoxification of MG dye, not just for its discoloration, since some works show false positives due to the formation of colorless intermediates such as leucomalachite. The optimal pH and temperature parameters of LacMeta were 5.0 and 50 °C, respectively (MG as substrate). LacMeta was tolerant of up to 10 mmol L−1 EDTA (82%) and up to 5% (V/V) acetone (91%) and methanol (71%), while SDS promoted severe inhibition. For ions, a high tolerance to cobalt, zinc, manganese, and calcium (10 mmol L−1 ) was also observed (>90%). Even under high-salinity conditions (1 mol L−1 NaCl), the residual bleaching activity of the dye remained at 61%. Furthermore, the bleaching product of MG did not inhibit the germination of sorghum and tomato seeds and was inert to the vegetative structures of the germinated seedlings. Additionally, this treatment effectively reduced the cytotoxic effect of the dye on microorganisms (Escherichia coli and Azospirillum brasilense), which can be explained by H-NMR spectral analysis results since LacMeta completely degraded the peak signals corresponding to the aromatic rings in the dye, demonstrating extreme efficiency in the bioremediation of the xenobiotic at high concentrations (50 mg L−1 ). MenosLaccases highlight for xenobiotic bioremediation, as well as application in the fine chemical, textile, biofuel and food industries. In a previous work, we described the preliminary characterization of laccase LacMeta, a promising enzyme for the bioremediation of dyes, able to decolorization malachite green (MG), trypan blue, methylene blue. Here we demonstrate that LacMeta is indeed suitable for the complete degradation and detoxification of MG dye, not just for its discoloration, since some works show false positives due to the formation of colorless intermediates such as leucomalachite. The optimal pH and temperature parameters of LacMeta were 5.0 and 50 °C, respectively (MG as substrate). LacMeta was tolerant of up to 10 mmol L−1 EDTA (82%) and up to 5% (V/V) acetone (91%) and methanol (71%), while SDS promoted severe inhibition. For ions, a high tolerance to cobalt, zinc, manganese, and calcium (10 mmol L−1 ) was also observed (>90%). Even under high-salinity conditions (1 mol L−1 NaCl), the residual bleaching activity of the dye remained at 61%. Furthermore, the bleaching product of MG did not inhibit the germination of sorghum and tomato seeds and was inert to the vegetative structures of the germinated seedlings. Additionally, this treatment effectively reduced the cytotoxic effect of the dye on microorganisms (Escherichia coli and Azospirillum brasilense), which can be explained by H-NMR spectral analysis results since LacMeta completely degraded t... Mostrar Tudo |
|
Palavras-Chave: |
Detoxification; Halotolerance ·; Triphenylmethane dye. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02417naa a2200229 a 4500
001 2153222
005 2023-04-18
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s11274-023-03572-w$2DOI
100 1 $aLIMA, N. S. M.
245 $aDynamics of the role of LacMeta laccase in the complete degradation and detoxification of malachite green.$h[electronic resource]
260 $c2023
300 $a1 - 13
520 $aLaccases highlight for xenobiotic bioremediation, as well as application in the fine chemical, textile, biofuel and food industries. In a previous work, we described the preliminary characterization of laccase LacMeta, a promising enzyme for the bioremediation of dyes, able to decolorization malachite green (MG), trypan blue, methylene blue. Here we demonstrate that LacMeta is indeed suitable for the complete degradation and detoxification of MG dye, not just for its discoloration, since some works show false positives due to the formation of colorless intermediates such as leucomalachite. The optimal pH and temperature parameters of LacMeta were 5.0 and 50 °C, respectively (MG as substrate). LacMeta was tolerant of up to 10 mmol L−1 EDTA (82%) and up to 5% (V/V) acetone (91%) and methanol (71%), while SDS promoted severe inhibition. For ions, a high tolerance to cobalt, zinc, manganese, and calcium (10 mmol L−1 ) was also observed (>90%). Even under high-salinity conditions (1 mol L−1 NaCl), the residual bleaching activity of the dye remained at 61%. Furthermore, the bleaching product of MG did not inhibit the germination of sorghum and tomato seeds and was inert to the vegetative structures of the germinated seedlings. Additionally, this treatment effectively reduced the cytotoxic effect of the dye on microorganisms (Escherichia coli and Azospirillum brasilense), which can be explained by H-NMR spectral analysis results since LacMeta completely degraded the peak signals corresponding to the aromatic rings in the dye, demonstrating extreme efficiency in the bioremediation of the xenobiotic at high concentrations (50 mg L−1 ).
653 $aDetoxification
653 $aHalotolerance ·
653 $aTriphenylmethane dye
700 1 $aGOMES-PEPE, E. S.
700 1 $aKOCK, F. V. C.
700 1 $aCOLNAGO, L. A.
700 1 $aLEMOS, E. G. M.
773 $tWorld Journal of Microbiology and Biotechnology$gv. 39, 127, 2023.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Instrumentação (CNPDIA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Roraima. |
|
Data corrente: |
14/04/2010 |
|
Data da última atualização: |
17/11/2022 |
|
Tipo da produção científica: |
Comunicado Técnico/Recomendações Técnicas |
|
Autoria: |
ZILLI, J. E.; SILVA NETO, M. L.; FRANÇA JÚNIOR, I.; PERIN, L.; MELO, A. R. |
|
Afiliação: |
JERRI EDSON ZILLI, CPAF-RR. |
|
Título: |
Resposta do feijão-caupi à inoculação com estirpes de Bradyrhizobium recomendadas para a soja. |
|
Ano de publicação: |
2009 |
|
Fonte/Imprenta: |
Boa Vista, RR: Embrapa Roraima, 2009. |
|
Páginas: |
5 p. |
|
Série: |
(Embrapa Roraima. Comunicado Técnico, 27). |
|
Idioma: |
Português |
|
Palavras-Chave: |
Feijão-caupi. |
|
Thesagro: |
Soja. |
|
Thesaurus NAL: |
Bradyrhizobium. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/695759/1/cot27-2009-caupi-zilli.pdf
|
|
Marc: |
LEADER 00606nam a2200205 a 4500
001 1695759
005 2022-11-17
008 2009 bl uuuu u0uu1 u #d
100 1 $aZILLI, J. E.
245 $aResposta do feijão-caupi à inoculação com estirpes de Bradyrhizobium recomendadas para a soja.
260 $aBoa Vista, RR: Embrapa Roraima$c2009
300 $a5 p.
490 $a(Embrapa Roraima. Comunicado Técnico, 27).
650 $aBradyrhizobium
650 $aSoja
653 $aFeijão-caupi
700 1 $aSILVA NETO, M. L.
700 1 $aFRANÇA JÚNIOR, I.
700 1 $aPERIN, L.
700 1 $aMELO, A. R.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Roraima (CPAF-RR) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
