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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
23/11/2022 |
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Data da última atualização: |
09/11/2023 |
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Tipo da produção científica: |
Orientação de Tese de Pós-Graduação |
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Autoria: |
ROMERO PELÁEZ, R. D. |
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Afiliação: |
RUBÉN DARIO ROMERO PELÁEZ, UNIVERSIDADE DE BRASÍLIA. |
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Título: |
Interactions between filamentous and yeast form fungi with biotechnological potential in the production of enzymes and metabolites. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
2022 |
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Páginas: |
156 f. |
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Idioma: |
Inglês |
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Notas: |
Thesis (Doctor in Microbial Biology) - University of Brasília, Brasília, DF. Advisor: João Ricardo Moreira de Almeida; Co-advisor: Félix Gonçalves de Siqueira, CNPAE. |
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Conteúdo: |
Co-cultures between microorganisms have shown important applications in various biotechnological processes, such as obtaining enzymes with high catalytic potential or prospecting for metabolites of commercial importance. This strategy is based on the molecular response to the interactions between two biological individuals, which may lead to increased enzymatic activities, secretion of bioactive compounds, or increased efficiency of plant biomass bioconversion. This thesis evaluates and describes the interactions with the filamentous fungi Panus lecomtei and Trichoderma reesei, and the yeast Sporidiobolus pararoseus as a function of the secretion of commercially important lignocellulolytic enzymes. The first stage of this study, addressed in Chapter I, involved the evaluation of the best conditions in the co-culture of P. lecomtei and T. reesei in submerged culture, where it was shown that temperature variations, the time between inocula, and medium composition characteristics define the production of an extract with high hydrolytic efficiency, as well as readily fermentable hydrolysates in ethanol. The proteomic analysis of the best condition for enzyme production in the co-culture of P. lecomtei and T. reesei, addressed in Chapter II, showed that the high hydrolysis efficiency might be related to the production of CAZy enzymes distributed in families with complete coverage of cellulases (endo-, exo- and betaglucosidases), in addition to various families associated with the degradation of hemicellulose and accessory enzymes such as lytic polysaccharide monooxygenases (LPMO). Finally, in Chapter III, it was shown that P. lecomtei responds positively in a coculture with S. pararoseus, increasing laccase production up to 2713.8 U/mL. This extract showed potential in the decolorization of RBBR industrial textile dye by about 80% and, even though this extract did not show significant cellulase activities. In addition, a cocktail with the extract from the best culture conditions of P. lecomtei and T. reesei allowed the highest percentage of hydrolytic efficiency among all conditions evaluated, with 75.69%, a novel result in the literature. This study demonstrates that the interactions between filamentous and yeast-like fungi, antagonistic or not, favor the generation of products with industrial potential. These may help in the future to solve problems related to the deconstruction of plant biomass or detoxification of polluted effluents. MenosCo-cultures between microorganisms have shown important applications in various biotechnological processes, such as obtaining enzymes with high catalytic potential or prospecting for metabolites of commercial importance. This strategy is based on the molecular response to the interactions between two biological individuals, which may lead to increased enzymatic activities, secretion of bioactive compounds, or increased efficiency of plant biomass bioconversion. This thesis evaluates and describes the interactions with the filamentous fungi Panus lecomtei and Trichoderma reesei, and the yeast Sporidiobolus pararoseus as a function of the secretion of commercially important lignocellulolytic enzymes. The first stage of this study, addressed in Chapter I, involved the evaluation of the best conditions in the co-culture of P. lecomtei and T. reesei in submerged culture, where it was shown that temperature variations, the time between inocula, and medium composition characteristics define the production of an extract with high hydrolytic efficiency, as well as readily fermentable hydrolysates in ethanol. The proteomic analysis of the best condition for enzyme production in the co-culture of P. lecomtei and T. reesei, addressed in Chapter II, showed that the high hydrolysis efficiency might be related to the production of CAZy enzymes distributed in families with complete coverage of cellulases (endo-, exo- and betaglucosidases), in addition to various families associated with the... Mostrar Tudo |
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Thesagro: |
Corante; Descoloração; Enzima Celulolítica. |
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Thesaurus Nal: |
Decolorization; Enzymatic hydrolysis; Lignocellulose. |
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Categoria do assunto: |
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Marc: |
LEADER 03255nam a2200205 a 4500
001 2148659
005 2023-11-09
008 2022 bl uuuu m 00u1 u #d
100 1 $aROMERO PELÁEZ, R. D.
245 $aInteractions between filamentous and yeast form fungi with biotechnological potential in the production of enzymes and metabolites.$h[electronic resource]
260 $a2022$c2022
300 $a156 f.
500 $aThesis (Doctor in Microbial Biology) - University of Brasília, Brasília, DF. Advisor: João Ricardo Moreira de Almeida; Co-advisor: Félix Gonçalves de Siqueira, CNPAE.
520 $aCo-cultures between microorganisms have shown important applications in various biotechnological processes, such as obtaining enzymes with high catalytic potential or prospecting for metabolites of commercial importance. This strategy is based on the molecular response to the interactions between two biological individuals, which may lead to increased enzymatic activities, secretion of bioactive compounds, or increased efficiency of plant biomass bioconversion. This thesis evaluates and describes the interactions with the filamentous fungi Panus lecomtei and Trichoderma reesei, and the yeast Sporidiobolus pararoseus as a function of the secretion of commercially important lignocellulolytic enzymes. The first stage of this study, addressed in Chapter I, involved the evaluation of the best conditions in the co-culture of P. lecomtei and T. reesei in submerged culture, where it was shown that temperature variations, the time between inocula, and medium composition characteristics define the production of an extract with high hydrolytic efficiency, as well as readily fermentable hydrolysates in ethanol. The proteomic analysis of the best condition for enzyme production in the co-culture of P. lecomtei and T. reesei, addressed in Chapter II, showed that the high hydrolysis efficiency might be related to the production of CAZy enzymes distributed in families with complete coverage of cellulases (endo-, exo- and betaglucosidases), in addition to various families associated with the degradation of hemicellulose and accessory enzymes such as lytic polysaccharide monooxygenases (LPMO). Finally, in Chapter III, it was shown that P. lecomtei responds positively in a coculture with S. pararoseus, increasing laccase production up to 2713.8 U/mL. This extract showed potential in the decolorization of RBBR industrial textile dye by about 80% and, even though this extract did not show significant cellulase activities. In addition, a cocktail with the extract from the best culture conditions of P. lecomtei and T. reesei allowed the highest percentage of hydrolytic efficiency among all conditions evaluated, with 75.69%, a novel result in the literature. This study demonstrates that the interactions between filamentous and yeast-like fungi, antagonistic or not, favor the generation of products with industrial potential. These may help in the future to solve problems related to the deconstruction of plant biomass or detoxification of polluted effluents.
650 $aDecolorization
650 $aEnzymatic hydrolysis
650 $aLignocellulose
650 $aCorante
650 $aDescoloração
650 $aEnzima Celulolítica
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| 1. |  | FERREIRA, V. C. de Q.; BELTRAO, N. E. de M.; LIMA, V. L. A. de; CENTENO, C. R. M. Sexualidade das flores do Pinhão manso adubado com farelo de mamona e irrigado com água residuária. In: CONGRESSO BRASILEIRO DE MAMONA, 5.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 2.; FÓRUM CAPIXABA DE PINHÃO-MANSO, 1., 2012, Guarapari. Desafios e Oportunidades: anais. Campina Grande: Embrapa Algodão, 2012. p. 101| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Algodão. |
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| 2. | | SANTOS, J. B. dos; AZEVEDO, C. A. V.; CAVALCANTE, L. F.; BELTRAO, N. E. de M.; SANTOS, D. B. dos; CENTENO, C. R. M. Desenvolvimento vegetativo da mamoneira em função da salinidade da água de irrigação. In: CONGRESSO BRASILEIRO DE MAMONA, 4.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 1., 2010, João Pessoa. Inclusão social e energia: anais. Campina Grande: Embrapa Algodão, 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Algodão. |
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| Registros recuperados : 2 | |
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