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Registro Completo |
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
14/10/2022 |
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Data da última atualização: |
14/10/2022 |
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Tipo da produção científica: |
Nota Técnica/Nota Científica |
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Autoria: |
NAKASU, E. Y. T.; SILVA, G.; MONTES, S. M. N. M.; MELLO, A. F. S. |
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Afiliação: |
ERICH YUKIO TEMPEL NAKASU, CNPH; GABRIELA SILVA, AGÊNCIA PAULISTA DE TECNOLOGIA DOS AGRONEGÓCIOS; SÔNIA M. N. M. MONTES, AGÊNCIA PAULISTA DE TECNOLOGIA DOS AGRONEGÓCIOS; ALEXANDRE FURTADO SILVEIRA MELLO, CNPH. |
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Título: |
Virome analysis of sweetpotato in three Brazilian regions using high-throughput sequencing. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Tropical Plant Pathology, Oct. 2022. |
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DOI: |
https://doi.org/10.1007/s40858-022-00532-x |
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Idioma: |
Inglês |
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Conteúdo: |
Sweet potato (Ipomoea batatas (L.) Lam.) is a highly nutritious food crop that suffers severe yield losses due to viral diseases. These are unintentionally spread mainly by vegetative propagation techniques commonly used by farmers. Virus indexing of vine cuttings (slips) coupled with tools specifically developed for identification of the main viruses is therefore essential to avoid disease outbreaks. In this work, we assessed the viral diversity in sweetpotatoes collected in some of the main producing areas in Brazil. The cuttings were grafted onto Ipomoea setosa Ker Gawl. prior to being subjected to ELISA for ten different viruses. Total DNA and RNA were also extracted from sweetpotato and I. setosa leaves for high-throughput sequencing (HTS) on an Illumina platform. Using this HTS strategy, nine viral species known to infect sweetpotatoes were identified, being seven of those species also identified in grafted I. setosa, two species identified exclusively in sweetpotato and two exclusively on I. setosa. From the ten different viruses tested by ELISA, six were identified in the samples evaluated. The generated data will be useful for virus indexing of sweetpotato as well as for virus resistance screening in sweetpotato breeding programs. |
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Thesagro: |
Batata Doce; Ipomoea Batatas; Variedade Resistente; Vírus. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
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Registro original: |
Embrapa Hortaliças (CNPH) |
