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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
30/08/2022 |
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Data da última atualização: |
30/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, E. C. A. de; SILVA, B. P.; PORTO, C.; PILAU, E. J.; MENEZES, C.; FLACH, A. |
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Afiliação: |
EDINEIDE CRISTINA ALEXANDRE DE SOUZA, UNIVERSIDADE FEDERAL DE RORAIMA; BEATRIZ PAES SILVA, UNIVERSIDADE ESTADUAL DE MARINGÁ; CARLA PORTO, MS BIOSCIENCE INCUBADORA TECNOLOGICA DE MARINGÁ; EDUARDO JORGE PILAU, UNIVERSIDADE ESTADUAL DE MARINGÁ; CRISTIANO MENEZES, CNPMA; ADRIANA FLACH, UNIVERSIDADE FEDERAL DE RORAIMA. |
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Título: |
Molecular network-guided chemical profile and mass spectrometry, volatile compounds, and antimicrobial activity of Scaptotrigona depilis propolis. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Rapid Communications in Mass Spectrometry, V. 36, N. 19, e9348, 202. |
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ISSN: |
0951-4198 |
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DOI: |
https://doi.org/10.1002/rcm.9348 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Rationale Propolis has a great diversity in its composition due to numerous factors; therefore, each study is an important contribution to the knoFwledge of its composition and biological action. The objective of this study was to determine the chemical profile and biological activity of propolis produced by Scaptotrigona depilis. Methods Extracts with 70% ethanol (EPE70) and with cereal alcohol (CAPE) were elaborated, and then characterized using UHPLC-ESI(+)-MS/MS. Volatile compounds were extracted and then characterized using gas chromatography mass spectrometry (GC?MS). In addition, antimicrobial activities were verified against resistant strains. Results The volatile compounds of propolis predominantly consist of sesquiterpenes. Using the exploratory metabolomic approach, compounds of different classes were putatively identified in the ethanolic extracts, of which the most representative were terpenes, and some of the sesquiterpenes identified among the volatiles were also detected. The extracts were shown to be active against Escherichia coli and Staphylococcus aureus bacteria with a minimum inhibitory concentration (MIC) of 0.5 and 1.0 mg?mL?1, respectively. Conclusions The molecular network approach proved to be determining the chemical profile of S. depilis propolis rapidly and accurately, and led to the identification of lipophilic compounds. The identification of compounds using GC?MS and UHPLC-ESI(+)-MS/MS is complementary and useful for the characterization of propolis. MenosAbstract: Rationale Propolis has a great diversity in its composition due to numerous factors; therefore, each study is an important contribution to the knoFwledge of its composition and biological action. The objective of this study was to determine the chemical profile and biological activity of propolis produced by Scaptotrigona depilis. Methods Extracts with 70% ethanol (EPE70) and with cereal alcohol (CAPE) were elaborated, and then characterized using UHPLC-ESI(+)-MS/MS. Volatile compounds were extracted and then characterized using gas chromatography mass spectrometry (GC?MS). In addition, antimicrobial activities were verified against resistant strains. Results The volatile compounds of propolis predominantly consist of sesquiterpenes. Using the exploratory metabolomic approach, compounds of different classes were putatively identified in the ethanolic extracts, of which the most representative were terpenes, and some of the sesquiterpenes identified among the volatiles were also detected. The extracts were shown to be active against Escherichia coli and Staphylococcus aureus bacteria with a minimum inhibitory concentration (MIC) of 0.5 and 1.0 mg?mL?1, respectively. Conclusions The molecular network approach proved to be determining the chemical profile of S. depilis propolis rapidly and accurately, and led to the identification of lipophilic compounds. The identification of compounds using GC?MS and UHPLC-ESI(+)-MS/MS is complementary and useful for the characteriz... Mostrar Tudo |
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Palavras-Chave: |
Abelha mandaguari. |
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Thesagro: |
Abelha Brasileira; Análise Química; Espectrometria; Própolis. |
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Thesaurus Nal: |
Antibacterial properties; Honey bees; Mass spectrometry; Scaptotrigona; Stingless bees; Terpenoids; Volatile compounds. |
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Categoria do assunto: |
O Insetos e Entomologia |
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Marc: |
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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| Registros recuperados : 815 | |
| 2. |  | LIMA, C. E. P. ìndice de qualidade do solo. In: GUEDES, I. M. R.; VIDAL, M. C. (Ed.). Curso internacional sobre produção sustentável de hortaliças: módulo 2 produção sustentável de hortaliças. Embrapa: Brasília, DF, 2012. p.22-23| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Hortaliças. |
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| 5. | | LIMA, C. E. P. Manejo e conservação do solo. In: GUEDES, I. M. R.; VIDAL, M. C. (Ed.). Curso internacional sobre produção sustentável de hortaliças: módulo 2 produção sustentável de hortaliças. Embrapa: Brasília, DF, 2012. p. 18-19.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Hortaliças. |
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| Registros recuperados : 815 | |
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