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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
23/02/2022 |
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Data da última atualização: |
23/02/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MORAES, I. C.; PEREIRA, W. A.; NANI, T. F.; PAULA, C. M. P. de; ROCHA, M. J.; SOUZA SOBRINHO, F. de; TECHIO, V. H. |
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Afiliação: |
ISABELLA DE CAMPOS MORAES, Universidade Federal de Lavras; WELISON ANDRADE PEREIRA, Universidade Federal de Lavras; THAÍS FURTADO NANI, Universidade Federal de Lavras; CRISTINA MARIA PINTO DE PAULA, Universidade Federal de Lavras; MARA JANE DA ROCHA, Universidade Federal de Lavras; FAUSTO DE SOUZA SOBRINHO, CNPGL; VANIA HELENA TECHIO, Universidade Federal de Lavras. |
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Título: |
Karyotype analysis and mode of reproduction of two species of Urochloa P. Beauv. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Crop Science. v. 61, p. 3415-3424, 2021. |
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DOI: |
https://doi.org/10.1002/csc2.20542 |
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Idioma: |
Inglês |
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Conteúdo: |
Grasses with desirable traits for agriculture and livestock production are continuallybeing sought. The genusUrochloacomprises many species of good forage poten-tial, although studies have mostly focused on commercial cultivars. Karyotype datacan be used to characterize species and generate information for taxonomy and evo-lutionary studies and contribute to breeding programs. The mode of reproductionis also an essential piece of information to determine breeding strategies. This studyaimed to characterize the karyotypes ofUrochloa arrecta(Hack. ex T. Dur. & Schniz)O. Morrone & F. Zuloaga and a pentaploid cytotype ofU. brizantha(Hochst. exA. Rich.) R. Webster, including the mapping of ribosomal DNA (rDNA) sites andnuclear DNA content and indicating the mode of reproduction. Slides were pre-pared using the flame drying technique and were subjected to fluorescent in situhybridization with 35S and 5S rDNA probes. DNA content was estimated by flowcytometry. The classification of the reproduction mode was based on the amplifica-tion of the primers N14 and p779/p780.Urochloa arrectapresented 2C=2.66 pgof DNA, karyotype formula 2n=4x=34 m+2sm, four chromosomes with 35SrDNA sites, and four other chromosomes with 5S rDNA sites. The absence of ampli-fication products with the primers N14 and p779/p780 provided evidence of sexualreproduction.Urochloa brizanthapresented 2C=4.52 pg of DNA and karyotypeformula 2n=5x=45 m. Five chromosomes presented 35S rDNA, and eight otherchromosomes displayed 5S rDNA sites. Polymerase chain reaction results indicatedthat this cytotype has an apomictic mode of reproduction. The organization of kary-otypes suggests an allopolyploid origin for both genotypes. Possible applications ofthe information found are discussed. MenosGrasses with desirable traits for agriculture and livestock production are continuallybeing sought. The genusUrochloacomprises many species of good forage poten-tial, although studies have mostly focused on commercial cultivars. Karyotype datacan be used to characterize species and generate information for taxonomy and evo-lutionary studies and contribute to breeding programs. The mode of reproductionis also an essential piece of information to determine breeding strategies. This studyaimed to characterize the karyotypes ofUrochloa arrecta(Hack. ex T. Dur. & Schniz)O. Morrone & F. Zuloaga and a pentaploid cytotype ofU. brizantha(Hochst. exA. Rich.) R. Webster, including the mapping of ribosomal DNA (rDNA) sites andnuclear DNA content and indicating the mode of reproduction. Slides were pre-pared using the flame drying technique and were subjected to fluorescent in situhybridization with 35S and 5S rDNA probes. DNA content was estimated by flowcytometry. The classification of the reproduction mode was based on the amplifica-tion of the primers N14 and p779/p780.Urochloa arrectapresented 2C=2.66 pgof DNA, karyotype formula 2n=4x=34 m+2sm, four chromosomes with 35SrDNA sites, and four other chromosomes with 5S rDNA sites. The absence of ampli-fication products with the primers N14 and p779/p780 provided evidence of sexualreproduction.Urochloa brizanthapresented 2C=4.52 pg of DNA and karyotypeformula 2n=5x=45 m. Five chromosomes presented 35S rDNA, and eight otherchromosomes dis... Mostrar Tudo |
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Thesagro: |
Cariótipo; Gramínea; Gramínea Forrageira; Reprodução Vegetal. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02509naa a2200253 a 4500
001 2140332
005 2022-02-23
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1002/csc2.20542$2DOI
100 1 $aMORAES, I. C.
245 $aKaryotype analysis and mode of reproduction of two species of Urochloa P. Beauv.$h[electronic resource]
260 $c2021
520 $aGrasses with desirable traits for agriculture and livestock production are continuallybeing sought. The genusUrochloacomprises many species of good forage poten-tial, although studies have mostly focused on commercial cultivars. Karyotype datacan be used to characterize species and generate information for taxonomy and evo-lutionary studies and contribute to breeding programs. The mode of reproductionis also an essential piece of information to determine breeding strategies. This studyaimed to characterize the karyotypes ofUrochloa arrecta(Hack. ex T. Dur. & Schniz)O. Morrone & F. Zuloaga and a pentaploid cytotype ofU. brizantha(Hochst. exA. Rich.) R. Webster, including the mapping of ribosomal DNA (rDNA) sites andnuclear DNA content and indicating the mode of reproduction. Slides were pre-pared using the flame drying technique and were subjected to fluorescent in situhybridization with 35S and 5S rDNA probes. DNA content was estimated by flowcytometry. The classification of the reproduction mode was based on the amplifica-tion of the primers N14 and p779/p780.Urochloa arrectapresented 2C=2.66 pgof DNA, karyotype formula 2n=4x=34 m+2sm, four chromosomes with 35SrDNA sites, and four other chromosomes with 5S rDNA sites. The absence of ampli-fication products with the primers N14 and p779/p780 provided evidence of sexualreproduction.Urochloa brizanthapresented 2C=4.52 pg of DNA and karyotypeformula 2n=5x=45 m. Five chromosomes presented 35S rDNA, and eight otherchromosomes displayed 5S rDNA sites. Polymerase chain reaction results indicatedthat this cytotype has an apomictic mode of reproduction. The organization of kary-otypes suggests an allopolyploid origin for both genotypes. Possible applications ofthe information found are discussed.
650 $aCariótipo
650 $aGramínea
650 $aGramínea Forrageira
650 $aReprodução Vegetal
700 1 $aPEREIRA, W. A.
700 1 $aNANI, T. F.
700 1 $aPAULA, C. M. P. de
700 1 $aROCHA, M. J.
700 1 $aSOUZA SOBRINHO, F. de
700 1 $aTECHIO, V. H.
773 $tCrop Science.$gv. 61, p. 3415-3424, 2021.
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