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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
17/11/2021 |
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Data da última atualização: |
10/01/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, J. D. R.; BATISTA, R. I. T. P.; UNGERFELD, R.; TAIRA, A. R.; ESPÍRITO SANTO, C. G. do; SOUZA-FABJAN, J. M. G.; FERNANDES, D. A. de M.; BALARO, M. F. A.; COSENTINO, I. O.; BRAIR, V. L.; PINTO, P. H. N.; CARVALHO, A. B. da S.; FONSECA, J. F. da; BRANDÃO, F. Z. |
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Afiliação: |
JULIANA DANTAS RODRIGUES SANTOS, UNIVERSIDADE FEDERAL FLUMINENSE; RIBRIO IVAN TAVARES PEREIRA BATISTA, UNIVERSIDADE FEDERAL FLUMINENSE; RODOLFO UNGERFELD, UNIVERSIDAD DE LA REPÚBLICA; AUGUSTO RYONOSUKE TAIRA, UNIVERSIDADE FEDERAL FLUMINENSE; CAROLINE GOMES DO ESPÍRITO SANTO, UNIVERSIDADE FEDERAL FLUMINENSE; JOANNA MARIA GONÇALVES SOUZA-FABJAN, UNIVERSIDADE FEDERAL FLUMINENSE; DANIEL ANDREWS DE MOURA FERNANDES, UNIVERSIDADE FEDERAL FLUMINENSE; MÁRIO FELIPE ALVAREZ BALARO, UNIVERSIDADE FEDERAL FLUMINENSE; ISABEL OLIVEIRA COSENTINO, UNIVERSIDADE FEDERAL FLUMINENSE; VIVIANE LOPES BRAIR, UNIVERSIDADE FEDERAL FLUMINENSE; PEDRO HENRIQUE NICOLAU PINTO, UNIVERSIDADE FEDERAL FLUMINENSE; ANA BEATRIZ DA SILVA CARVALHO, UNIVERSIDADE FEDERAL FLUMINENSE; JEFERSON FERREIRA DA FONSECA, CNPC; FELIPE ZANDONADI BRANDÃO, UNIVERSIDADE FEDERAL FLUMINENSE. |
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Título: |
Hormonal protocol used for cervical dilation in ewes does not affect morphological embryo quality but reduces recovery rate and temporarily alters gene expression. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Veterinary Record, v. 190, n. 5, e1064, Mar. 2021. |
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DOI: |
https://doi.org/10.1002/vetr.1064 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Background: Information on the impact of hormonal protocols for cervical dilation on the quality of ovine embryos is scarce. Methods: To compare the quality of embryos after cervical dilation protocol, ewes (n = 64) were allocated into either a treated group (100 ?g estradiol benzoate intravenous and 0.12 mg cloprostenol intramuscularly, 12 hours before embryo collection plus 100 iu oxytocin intravenous 15 minutes before the collection procedure) or a control group (saline). Luteal function was analysed using ultrasonography and P4 measurement. Some collected embryos were frozen/thawed for gene expression, others were cultured in vitro, frozen/thawed for gene expression, and the remaining embryos were fixed for the apoptosis test (TUNEL test). Results: The treatment reduced fluid (p=0.04) and structure (p=0.03) recovery rates, but the morphological quality, development stage, and apoptosis incidence of the embryos were not affected by treatment. The corpora lutea of the control group had greater blood perfusion (p = 0.002) and greater P4 concentrations at 6, 9, and 12 h after the treatment (p < 0.0001). The expression of BAX, BCL2, PRDX1, and HSP90 genes were not affected by the treatment. However, the embryos in the treated group had fewer NANOG and OCT4 transcripts than control embryos (p = 0.008; p = 0.006, respectively). After culture, there was no difference between the groups in any gene. Conclusion: The hormonal protocol for cervical dilation reduced the efficiency of embryo collection. In addition, the treatment induced luteolysis and a transient alteration of embryo gene expression, however there were no detectable changes in embryo morphological quality, development stage, or incidence of apoptosis. MenosAbstract: Background: Information on the impact of hormonal protocols for cervical dilation on the quality of ovine embryos is scarce. Methods: To compare the quality of embryos after cervical dilation protocol, ewes (n = 64) were allocated into either a treated group (100 ?g estradiol benzoate intravenous and 0.12 mg cloprostenol intramuscularly, 12 hours before embryo collection plus 100 iu oxytocin intravenous 15 minutes before the collection procedure) or a control group (saline). Luteal function was analysed using ultrasonography and P4 measurement. Some collected embryos were frozen/thawed for gene expression, others were cultured in vitro, frozen/thawed for gene expression, and the remaining embryos were fixed for the apoptosis test (TUNEL test). Results: The treatment reduced fluid (p=0.04) and structure (p=0.03) recovery rates, but the morphological quality, development stage, and apoptosis incidence of the embryos were not affected by treatment. The corpora lutea of the control group had greater blood perfusion (p = 0.002) and greater P4 concentrations at 6, 9, and 12 h after the treatment (p < 0.0001). The expression of BAX, BCL2, PRDX1, and HSP90 genes were not affected by the treatment. However, the embryos in the treated group had fewer NANOG and OCT4 transcripts than control embryos (p = 0.008; p = 0.006, respectively). After culture, there was no difference between the groups in any gene. Conclusion: The hormonal protocol for cervical dilation reduced the eff... Mostrar Tudo |
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Palavras-Chave: |
Embryo production; QRT-PCR. |
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Thesaurus Nal: |
Cloprostenol; Corpus luteum; Embryo transfer; Females; Gene expression; Pharmacology; Progesterone; Reproduction; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03029naa a2200421 a 4500
001 2136205
005 2025-01-10
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1002/vetr.1064$2DOI
100 1 $aSANTOS, J. D. R.
245 $aHormonal protocol used for cervical dilation in ewes does not affect morphological embryo quality but reduces recovery rate and temporarily alters gene expression.$h[electronic resource]
260 $c2021
520 $aAbstract: Background: Information on the impact of hormonal protocols for cervical dilation on the quality of ovine embryos is scarce. Methods: To compare the quality of embryos after cervical dilation protocol, ewes (n = 64) were allocated into either a treated group (100 ?g estradiol benzoate intravenous and 0.12 mg cloprostenol intramuscularly, 12 hours before embryo collection plus 100 iu oxytocin intravenous 15 minutes before the collection procedure) or a control group (saline). Luteal function was analysed using ultrasonography and P4 measurement. Some collected embryos were frozen/thawed for gene expression, others were cultured in vitro, frozen/thawed for gene expression, and the remaining embryos were fixed for the apoptosis test (TUNEL test). Results: The treatment reduced fluid (p=0.04) and structure (p=0.03) recovery rates, but the morphological quality, development stage, and apoptosis incidence of the embryos were not affected by treatment. The corpora lutea of the control group had greater blood perfusion (p = 0.002) and greater P4 concentrations at 6, 9, and 12 h after the treatment (p < 0.0001). The expression of BAX, BCL2, PRDX1, and HSP90 genes were not affected by the treatment. However, the embryos in the treated group had fewer NANOG and OCT4 transcripts than control embryos (p = 0.008; p = 0.006, respectively). After culture, there was no difference between the groups in any gene. Conclusion: The hormonal protocol for cervical dilation reduced the efficiency of embryo collection. In addition, the treatment induced luteolysis and a transient alteration of embryo gene expression, however there were no detectable changes in embryo morphological quality, development stage, or incidence of apoptosis.
650 $aCloprostenol
650 $aCorpus luteum
650 $aEmbryo transfer
650 $aFemales
650 $aGene expression
650 $aPharmacology
650 $aProgesterone
650 $aReproduction
650 $aSheep
653 $aEmbryo production
653 $aQRT-PCR
700 1 $aBATISTA, R. I. T. P.
700 1 $aUNGERFELD, R.
700 1 $aTAIRA, A. R.
700 1 $aESPÍRITO SANTO, C. G. do
700 1 $aSOUZA-FABJAN, J. M. G.
700 1 $aFERNANDES, D. A. de M.
700 1 $aBALARO, M. F. A.
700 1 $aCOSENTINO, I. O.
700 1 $aBRAIR, V. L.
700 1 $aPINTO, P. H. N.
700 1 $aCARVALHO, A. B. da S.
700 1 $aFONSECA, J. F. da
700 1 $aBRANDÃO, F. Z.
773 $tVeterinary Record$gv. 190, n. 5, e1064, Mar. 2021.
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Embrapa Caprinos e Ovinos (CNPC) |
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| 6. |  | CAIXETA, E. T.; PESTANA, K. N.; PESTANA, R. K. N. Melhoramento do cafeeiro: ênfase na aplicação dos marcadores moleculares. In: GARCIA, G. de O.; REIS, E. F. dos; LIMA, J. S. de S.; XAVIER, A. C.; RODRIGUES, W. N. (Org.). Tópicos Especiais em produção vegetal V. Alegre: CCAUFES, 2015, v. 1| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Café. |
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| 10. | | SANTANA, M. F.; ZAMBOLIM, E. M.; OLIVEIRA, L. O. de; CAIXETA, E. T.; ZAMBOLIM, L. Análise molecular do rDNA de Hemileia vastatrix. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 5., 2007, Águas de Lindóia, SP. Anais... Brasília, DF: Embrapa Café, 2007.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 11. | | FREITAS, R. L.; ZAMBOLIM, E. M.; LELIS, D. T.; CAIXETA, E. T.; ZAMBOLIM, L. Colletotrichum gloeosporioides E C. boninense associados à antracnose do café no Brasil. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 7., 2011, Araxá. Anais... Brasília, DF: Embrapa Café, 2011.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 16. | | BARREIROS, P. R. R. M.; PRADO, R. L.; ZAMBOLIM, L.; SAKIYAMA, N. S.; CAIXETA, E. T. Busca e análise de transposons no genoma de Hemileia vastatrix. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 9., 2017, Foz de Iguaçu. Melhoramento de plantas: projetando o futuro. Maringá, PR: SBMP, 2017. p. 36| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 17. | | MACHADO, G. M.; CAIXETA, E. S.; FRANCO, M. M.; RUMPF, R.; DODE, M. A. N. Cultivo embrionário In vitro pós-eclosão até D15: desenvolvimento e sexo de embriões bovinos. Acta Scientiae Veterinariae, v. 36, supl. 2, p. S508, 2008. Edição dos resumos da 22. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Guarujá, SP, 2008. Apresentado também no: In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 13., 2008, Brasília,...| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 20. | | MAIA, T. A.; ZAMBOLIM, E. M.; CAIXETA, E. T.; MISSIO, R. F.; ZAMBOLIM, L. Diversidade genética de Hemileia vastatrix utilizando marcador molecular AFLP. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 5., 2007, Águas de Lindóia. Anais... Brasília, DF: Embrapa Café, 2007.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| Registros recuperados : 223 | |
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