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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
05/11/2021 |
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Data da última atualização: |
05/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OKINO, C. H.; CAMINOTTO, L. C. F.; KAPRITCHKOFF, R. T. I.; NICIURA, S. C. M.; ESTEVES, S. N.; SANTOS, I. B. DOS; TOSCANO, J. H. B.; OLIVEIRA, M. C. de S.; CHAGAS, A. C. de S. |
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Afiliação: |
CINTIA HIROMI OKINO, CPPSE; LARA CRISTINA FRIZONI CAMINOTTO, UNICEP; RAFAELA TAMI IKEDA KAPRITCHKOFF, UNESP; SIMONE CRISTINA MEO NICIURA, CPPSE; SERGIO NOVITA ESTEVES, CPPSE; ISABELLA BARBOSA DOS SANTOS, UNESP; JOAO HENRIQUE BARBOSA TOSCANO, UNESP; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
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Título: |
Comparison of ovine B-globin haplotype sequences and a new multiplex PCR for identification. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Veterinary Parasitology, v.300, 109592, dec. 2021. |
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Páginas: |
5 p. |
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DOI: |
https://doi.org/10.1016/j.vetpar.2021.109592 |
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Idioma: |
Inglês |
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Conteúdo: |
Deletion of pre-adult BC-globin in sheep harboring BB haplotype of B-globin was associated to decreased tolerance to anemia and hypoxia, and consequently, reduced resistance to Haemonchus contortus infection, which is closely related to severe anemia. Recently, a qPCR using hydrolysis probe was successfully developed for B-globin haplotype identification, and association between resistance against H. contortus and presence of ?A allele was observed in Morada Nova sheep. Thus, this study aimed to better investigate the differences between B-globin haplotypes and to develop a conventional multiplex PCR, as an alternative to qPCR assay for B-globin haplotype identification. A total of 333 Morada Nova lambs had their blood collected and tested by both qPCR and new multiplex PCR, and 100 % of agreement was observed between the results. Since different primers were designed for such assay development, including different target genes, high specificity of both methods may be also highlighted. Three A haplotype samples were submitted to DNA Sanger sequencing of B-globin gene and compared to sequences previously deposited in Genbank. One nucleotide deletion in intronic region was observed only in AA haplotype of Morada Nova animals, while in BB animals the nucleotide remained present. To the best of our knowledge, this is the first report of multiplex conventional PCR for ovine B-globin haplotype identification. The advantages of the developed conventional PCR are reduced reagents costs (less than a half price) and wider reachability, since even labs without real time PCR thermocyclers are able to offer this assay. Therefore, it may become an important tool for sheep producers to improve genetic selection of parasite resistant animals. MenosDeletion of pre-adult BC-globin in sheep harboring BB haplotype of B-globin was associated to decreased tolerance to anemia and hypoxia, and consequently, reduced resistance to Haemonchus contortus infection, which is closely related to severe anemia. Recently, a qPCR using hydrolysis probe was successfully developed for B-globin haplotype identification, and association between resistance against H. contortus and presence of ?A allele was observed in Morada Nova sheep. Thus, this study aimed to better investigate the differences between B-globin haplotypes and to develop a conventional multiplex PCR, as an alternative to qPCR assay for B-globin haplotype identification. A total of 333 Morada Nova lambs had their blood collected and tested by both qPCR and new multiplex PCR, and 100 % of agreement was observed between the results. Since different primers were designed for such assay development, including different target genes, high specificity of both methods may be also highlighted. Three A haplotype samples were submitted to DNA Sanger sequencing of B-globin gene and compared to sequences previously deposited in Genbank. One nucleotide deletion in intronic region was observed only in AA haplotype of Morada Nova animals, while in BB animals the nucleotide remained present. To the best of our knowledge, this is the first report of multiplex conventional PCR for ovine B-globin haplotype identification. The advantages of the developed conventional PCR are reduced reagents co... Mostrar Tudo |
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Palavras-Chave: |
Allele; B haplotype; Beta globin C; Morada Nova; Resistance. |
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Thesaurus Nal: |
Sheep. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02671naa a2200313 a 4500
001 2135834
005 2021-11-05
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.vetpar.2021.109592$2DOI
100 1 $aOKINO, C. H.
245 $aComparison of ovine B-globin haplotype sequences and a new multiplex PCR for identification.$h[electronic resource]
260 $c2021
300 $a5 p.
520 $aDeletion of pre-adult BC-globin in sheep harboring BB haplotype of B-globin was associated to decreased tolerance to anemia and hypoxia, and consequently, reduced resistance to Haemonchus contortus infection, which is closely related to severe anemia. Recently, a qPCR using hydrolysis probe was successfully developed for B-globin haplotype identification, and association between resistance against H. contortus and presence of ?A allele was observed in Morada Nova sheep. Thus, this study aimed to better investigate the differences between B-globin haplotypes and to develop a conventional multiplex PCR, as an alternative to qPCR assay for B-globin haplotype identification. A total of 333 Morada Nova lambs had their blood collected and tested by both qPCR and new multiplex PCR, and 100 % of agreement was observed between the results. Since different primers were designed for such assay development, including different target genes, high specificity of both methods may be also highlighted. Three A haplotype samples were submitted to DNA Sanger sequencing of B-globin gene and compared to sequences previously deposited in Genbank. One nucleotide deletion in intronic region was observed only in AA haplotype of Morada Nova animals, while in BB animals the nucleotide remained present. To the best of our knowledge, this is the first report of multiplex conventional PCR for ovine B-globin haplotype identification. The advantages of the developed conventional PCR are reduced reagents costs (less than a half price) and wider reachability, since even labs without real time PCR thermocyclers are able to offer this assay. Therefore, it may become an important tool for sheep producers to improve genetic selection of parasite resistant animals.
650 $aSheep
653 $aAllele
653 $aB haplotype
653 $aBeta globin C
653 $aMorada Nova
653 $aResistance
700 1 $aCAMINOTTO, L. C. F.
700 1 $aKAPRITCHKOFF, R. T. I.
700 1 $aNICIURA, S. C. M.
700 1 $aESTEVES, S. N.
700 1 $aSANTOS, I. B. DOS
700 1 $aTOSCANO, J. H. B.
700 1 $aOLIVEIRA, M. C. de S.
700 1 $aCHAGAS, A. C. de S.
773 $tVeterinary Parasitology$gv.300, 109592, dec. 2021.
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| 1. |  | ALMEIDA, L. da S. S. de; LAMEIRA, O. A.; ASSIS, R. M. A. de; MOURA, R. C.; FERNANDES, V. S.; SOUSA, S. B. P. Aspectos fenológicos da quina - Quassia amara L. (Simaroubaceae). In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA, 20.; SEMINÁRIO DE PÓS-GRADUAÇÃO DA EMBRAPA AMAZÔNIA ORIENTAL, 4., 2016, Belém, PA. Anais. Belém, PA: Embrapa Amazônia Oriental, 2016. p. 133-136.| Tipo: Artigo em Anais de Congresso |
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