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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
12/09/2021 |
Data da última atualização: |
15/09/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ANTINARELLI, L. M. R.; SILVA, M. R.; GUIMARÃES, R. J. de P. S. e; TERROR, M. S.; LIMA, P. E.; ISHII, J. de S. C.; MUNIZ, P. F.; COIMBRA, E. S. |
Afiliação: |
LUCIANA MARIA RIBEIRO ANTINARELLI, Pós-graduanda, Universidade Federal de Minas Gerais; MARCIO ROBERTO SILVA, CNPGL; RICARDO JOSÉ DE PAULA SOUSA E GUIMARÃES, Instituto Evandro Chagas; MARIANA SEQUETTO TERROR, Universidade Federal de Juiz de Fora; PATRÍCIA ENHAM LIMA, Prefeitura de Juiz de Fora; JOELMA DE SOUZA COIMBRA ISHII, Prefeitura de Juiz de Fora; PATRÍCIA FABIANO MUNIZ, Prefeitura de Juiz de Fora; ELAINE SOARES COIMBRA, Universidade Federal de Juiz de Fora. |
Título: |
Rural residence remains a risk factor for Toxoplasma infection among pregnant women in a highly urbanized Brazilian area: a robust cross-sectional study. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Transactions of the Royal Society of Tropical Medicine and Hygiene, v. 115, p. 896-903, 2021. |
DOI: |
https://doi.org/10.1093/trstmh/traa153 |
Idioma: |
Inglês |
Conteúdo: |
Background: Despite high seroprevalence of asymptomatic infection in humans, toxoplasmosis can manifest as a severe systemic disease, as occurs in the congenital infection. Here we evaluate the seroprevalence of Toxoplasma infection among pregnant women in a highly urbanized area of Brazil. Methods: A robust seroepidemiological study was conducted using laboratory databases of anti-Toxoplasma gondii serological results together with information on age, month/year of diagnosis and place of residence of pregnant women in the public health system of the city of Juiz de Fora, Brazil. Results: Of 5895 pregnant women analysed, 54.7% showed seronegativity and 44.4% showed seropositivity for immunoglobulin G (IgG) antibodies against Toxoplasma gondii. This seropositivity rate increased to 68.3% when only considering participants from rural areas. Multivariate analysis revealed higher odds of being seropositive associated with age (odds ratio [OR] 1.06 [confidence interval {CI} 1.05 to 1.07]) and with living in rural areas (OR 2.96 [CI 1.64 to 5.36]). The spatial distribution of IgG seropositivity indicated a higher prevalence concentrated in rural and peripheral neighbourhoods. Conclusions: This is the first report to use spatial analysis to show a cluster of Toxoplasma infection in rural and peripheral neighbourhoods of a highly urbanized municipality, which highlights the need for adequate healthcare actions to be implemented for women living in these areas. |
Palavras-Chave: |
Anticorpos IgM e IgG; Área rural; Grávida; Soroprevalência. |
Thesagro: |
Doença; Toxoplasma Gondii; Toxoplasmose. |
Thesaurus Nal: |
Pregnant women; Rural areas; Seroprevalence; Toxoplasmosis. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 02594naa a2200349 a 4500 001 2134246 005 2021-09-15 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1093/trstmh/traa153$2DOI 100 1 $aANTINARELLI, L. M. R. 245 $aRural residence remains a risk factor for Toxoplasma infection among pregnant women in a highly urbanized Brazilian area$ba robust cross-sectional study.$h[electronic resource] 260 $c2021 520 $aBackground: Despite high seroprevalence of asymptomatic infection in humans, toxoplasmosis can manifest as a severe systemic disease, as occurs in the congenital infection. Here we evaluate the seroprevalence of Toxoplasma infection among pregnant women in a highly urbanized area of Brazil. Methods: A robust seroepidemiological study was conducted using laboratory databases of anti-Toxoplasma gondii serological results together with information on age, month/year of diagnosis and place of residence of pregnant women in the public health system of the city of Juiz de Fora, Brazil. Results: Of 5895 pregnant women analysed, 54.7% showed seronegativity and 44.4% showed seropositivity for immunoglobulin G (IgG) antibodies against Toxoplasma gondii. This seropositivity rate increased to 68.3% when only considering participants from rural areas. Multivariate analysis revealed higher odds of being seropositive associated with age (odds ratio [OR] 1.06 [confidence interval {CI} 1.05 to 1.07]) and with living in rural areas (OR 2.96 [CI 1.64 to 5.36]). The spatial distribution of IgG seropositivity indicated a higher prevalence concentrated in rural and peripheral neighbourhoods. Conclusions: This is the first report to use spatial analysis to show a cluster of Toxoplasma infection in rural and peripheral neighbourhoods of a highly urbanized municipality, which highlights the need for adequate healthcare actions to be implemented for women living in these areas. 650 $aPregnant women 650 $aRural areas 650 $aSeroprevalence 650 $aToxoplasmosis 650 $aDoença 650 $aToxoplasma Gondii 650 $aToxoplasmose 653 $aAnticorpos IgM e IgG 653 $aÁrea rural 653 $aGrávida 653 $aSoroprevalência 700 1 $aSILVA, M. R. 700 1 $aGUIMARÃES, R. J. de P. S. e 700 1 $aTERROR, M. S. 700 1 $aLIMA, P. E. 700 1 $aISHII, J. de S. C. 700 1 $aMUNIZ, P. F. 700 1 $aCOIMBRA, E. S. 773 $tTransactions of the Royal Society of Tropical Medicine and Hygiene$gv. 115, p. 896-903, 2021.
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Embrapa Gado de Leite (CNPGL) |
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Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
29/11/2013 |
Data da última atualização: |
20/01/2014 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 4 |
Autoria: |
CARVALHO, T. C. de; KRZYZANOWSKI, F. C.; OHLSON, O. de C.; PANOBIANCO, M. |
Afiliação: |
TEREZA CRISTINA DE CARVALHO, UFPR; FRANCISCO CARLOS KRZYZANOWSKI, CNPSO; OSVALDO DE CASTRO OHLSON, CLASPAR; MARISTELA PANOBIANCO, UFPR. |
Título: |
Tetrazolium test adjustment for wheat seeds. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Journal of Seed Science, Viçosa, MG, v. 35, n. 3, p. 361-367, 2013. |
DOI: |
10.1590/S2317-15372013000300013 |
Idioma: |
Inglês |
Conteúdo: |
ABSTRACT: The assessment of the germination test in wheat seeds varies from 4 to 15 days, because the species normally presents dormancy in freshly harvested seeds. The tetrazolium test can characterize seed viability in less than 24 hours including lots with dormancy seeds. The objective of this study was to develop a practical and efficient procedure for evaluating the viability of wheat seeds using the tetrazolium test. Five seed lots of the BRS 208 cultivar were used, where the following were tested: a) pre-conditioning between moist paper towels or direct immersion in water for 18 hours, at 20 °C; b) longitudinal section of the embryo and the endosperm; c) coloration on paper or by immersion for 2 and 3 hours, at 30 and 40 °C; and d) concentrations of tetrazolium solution at 0.075%, 0.1%, 0.5% and 1.0%. The tetrazolium test may be efficiently used to evaluate wheat seed viability by pre-conditioning the seeds between paper towels (18 hours, at 20 °C) and adopting the following combinations of preparation and coloration: coloration of both halves of the seed on paper (2 hours, at 30 °C), in a 1.0% tetrazolium solution; or coloration of one half of the seed by immersion (3 hours, at 30 °C), in a 0.1% tetrazolium solution; or coloration of one half of the seed by immersion (2 hours, at 40 °C), in a 0.075% tetrazolium solution. This latter procedure is recommended for identifying and discarding lots with lower viability. RESUMO: A avaliação do teste de germinação em sementes de trigo varia de 4 a 15 dias, uma vez que a espécie normalmente apresenta dormência em sementes recém-colhidas. O teste de tetrazólio pode caracterizar a viabilidade das sementes em menos de 24 horas, inclusive para lotes com sementes dormentes. O trabalho objetivou propor procedimento prático e eficiente para avaliação da viabilidade de sementes de trigo pelo teste de tetrazólio. Utilizaram-se cinco lotes de sementes da cultivar BRS 208, testando-se: a) pré-condicionamento entre papel toalha umedecido ou por imersão direta em água, por 18 horas, a 20 °C; b) corte em bissecção longitudinal ao longo do embrião e do endosperma; c) coloração sobre papel ou por imersão, durante 2 e 3 horas, a 30 e 40 °C; e d) concentrações de solução de tetrazólio a 0,075%, 0,1%, 0,5% e 1,0%. O teste de tetrazólio pode ser empregado com eficiência para avaliação da viabilidade de sementes de trigo, por meio do pré-condicionamento das sementes entre papel (18 horas, a 20 °C), adotando-se as seguintes combinações de preparo e coloração: coloração das duas metades da semente sobre papel (2 horas a 30 °C), em solução de tetrazólio a 1,0%; ou coloração de uma das metades da semente por imersão (3 horas a 30 °C), em solução de tetrazólio a 0,1%; ou coloração de uma das metades da semente por imersão (2 horas a 40 °C), em solução de tetrazólio a 0,075%, sendo este procedimento indicado para a identificação e descarte de lotes de viabilidade inferior. MenosABSTRACT: The assessment of the germination test in wheat seeds varies from 4 to 15 days, because the species normally presents dormancy in freshly harvested seeds. The tetrazolium test can characterize seed viability in less than 24 hours including lots with dormancy seeds. The objective of this study was to develop a practical and efficient procedure for evaluating the viability of wheat seeds using the tetrazolium test. Five seed lots of the BRS 208 cultivar were used, where the following were tested: a) pre-conditioning between moist paper towels or direct immersion in water for 18 hours, at 20 °C; b) longitudinal section of the embryo and the endosperm; c) coloration on paper or by immersion for 2 and 3 hours, at 30 and 40 °C; and d) concentrations of tetrazolium solution at 0.075%, 0.1%, 0.5% and 1.0%. The tetrazolium test may be efficiently used to evaluate wheat seed viability by pre-conditioning the seeds between paper towels (18 hours, at 20 °C) and adopting the following combinations of preparation and coloration: coloration of both halves of the seed on paper (2 hours, at 30 °C), in a 1.0% tetrazolium solution; or coloration of one half of the seed by immersion (3 hours, at 30 °C), in a 0.1% tetrazolium solution; or coloration of one half of the seed by immersion (2 hours, at 40 °C), in a 0.075% tetrazolium solution. This latter procedure is recommended for identifying and discarding lots with lower viability. RESUMO: A avaliação do teste de germinação em semente... Mostrar Tudo |
Thesagro: |
Germinação; Semente; Teste de sementes; Trigo. |
Thesaurus NAL: |
Seed germination; Wheat. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/93344/1/Tetrazolium-test-adjustment-for-wheat-seeds.pdf
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Marc: |
LEADER 03672naa a2200241 a 4500 001 1972565 005 2014-01-20 008 2013 bl uuuu u00u1 u #d 024 7 $a10.1590/S2317-15372013000300013$2DOI 100 1 $aCARVALHO, T. C. de 245 $aTetrazolium test adjustment for wheat seeds. 260 $c2013 520 $aABSTRACT: The assessment of the germination test in wheat seeds varies from 4 to 15 days, because the species normally presents dormancy in freshly harvested seeds. The tetrazolium test can characterize seed viability in less than 24 hours including lots with dormancy seeds. The objective of this study was to develop a practical and efficient procedure for evaluating the viability of wheat seeds using the tetrazolium test. Five seed lots of the BRS 208 cultivar were used, where the following were tested: a) pre-conditioning between moist paper towels or direct immersion in water for 18 hours, at 20 °C; b) longitudinal section of the embryo and the endosperm; c) coloration on paper or by immersion for 2 and 3 hours, at 30 and 40 °C; and d) concentrations of tetrazolium solution at 0.075%, 0.1%, 0.5% and 1.0%. The tetrazolium test may be efficiently used to evaluate wheat seed viability by pre-conditioning the seeds between paper towels (18 hours, at 20 °C) and adopting the following combinations of preparation and coloration: coloration of both halves of the seed on paper (2 hours, at 30 °C), in a 1.0% tetrazolium solution; or coloration of one half of the seed by immersion (3 hours, at 30 °C), in a 0.1% tetrazolium solution; or coloration of one half of the seed by immersion (2 hours, at 40 °C), in a 0.075% tetrazolium solution. This latter procedure is recommended for identifying and discarding lots with lower viability. RESUMO: A avaliação do teste de germinação em sementes de trigo varia de 4 a 15 dias, uma vez que a espécie normalmente apresenta dormência em sementes recém-colhidas. O teste de tetrazólio pode caracterizar a viabilidade das sementes em menos de 24 horas, inclusive para lotes com sementes dormentes. O trabalho objetivou propor procedimento prático e eficiente para avaliação da viabilidade de sementes de trigo pelo teste de tetrazólio. Utilizaram-se cinco lotes de sementes da cultivar BRS 208, testando-se: a) pré-condicionamento entre papel toalha umedecido ou por imersão direta em água, por 18 horas, a 20 °C; b) corte em bissecção longitudinal ao longo do embrião e do endosperma; c) coloração sobre papel ou por imersão, durante 2 e 3 horas, a 30 e 40 °C; e d) concentrações de solução de tetrazólio a 0,075%, 0,1%, 0,5% e 1,0%. O teste de tetrazólio pode ser empregado com eficiência para avaliação da viabilidade de sementes de trigo, por meio do pré-condicionamento das sementes entre papel (18 horas, a 20 °C), adotando-se as seguintes combinações de preparo e coloração: coloração das duas metades da semente sobre papel (2 horas a 30 °C), em solução de tetrazólio a 1,0%; ou coloração de uma das metades da semente por imersão (3 horas a 30 °C), em solução de tetrazólio a 0,1%; ou coloração de uma das metades da semente por imersão (2 horas a 40 °C), em solução de tetrazólio a 0,075%, sendo este procedimento indicado para a identificação e descarte de lotes de viabilidade inferior. 650 $aSeed germination 650 $aWheat 650 $aGerminação 650 $aSemente 650 $aTeste de sementes 650 $aTrigo 700 1 $aKRZYZANOWSKI, F. C. 700 1 $aOHLSON, O. de C. 700 1 $aPANOBIANCO, M. 773 $tJournal of Seed Science, Viçosa, MG$gv. 35, n. 3, p. 361-367, 2013.
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