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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
09/09/2021 |
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Data da última atualização: |
10/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA-FABJAN, J. M. G.; BATISTA, R. I. T. P.; MELO, L. M.; OLIVEIRA, M. A.; CHAVES, M. S.; FONSECA, J. F. da; FREITAS, V. J. F. |
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Afiliação: |
JOANNA M. G. SOUZA-FABJAN, Universidade Federal Fluminense (UFF) -Niterói, RJ, Brazil.; RIBRIO I. T. P. BATISTA, Universidade Federal Fluminense (UFF) -Niterói, RJ, Brazil.; LUCIANA M. MELO, Molecular Genetics Research Unit, University Center Fametro - Fortaleza, CE, Brazil.; MARCOS A. L. OLIVEIRA, Universidade Federal Rural de Pernambuco (UFRPE) - Recife, PE, Brazil.; MAIANA S. CHAVES; JEFERSON FERREIRA DA FONSECA, CNPC; VICENTE J. F. FREITAS, State University of Ceará (UFC) - Fortaleza, CE, Brazil. |
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Título: |
Transcervical versus laparotomy embryo recovery: what strategy is best for embryo bank formation in the Caninde goat conservation program? |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Biopreservation and Biobanking, v. 20, n. 2, p. 204-207, Sept. 2021. |
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DOI: |
http://doi.org/10.1089/bio.2021.0041 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: By allowing for the creation of embryo banks, reproductive biotechnologies play an essential role in the preservation of endangered goat breeds' genetic diversity. This study focused on comparing both available embryo collection methods [laparotomy (LAP) vs. nonsurgical embryo recovery (NSER)] in Canindé goats to create an embryo bank for later use in a breed conservation program. Twelve females were superovulated and subjected to either the LAP or NSER technique for embryo recovery. The recovery rate was similar (p>0.05) between NSER (86.8% ± 5.6%) and LAP (92.8% ± 4.0%). Moreover, there were no differences (p > 0.05) in the number of structures recovered, the viable embryos, and the freezable embryos per goat, respectively, for NSER (11.7 ± 1.3, 11.2 ± 1.5, and 10.2 ± 1.1) and LAP (10.3 ± 1.0, 8.7 ± 0.7, and 8.0 ± 0.8). Overall, 132 structures were collected out of 151 ovulations ( 12.6 ± 1.2 corpora lutea per goat). Finally, the procedure duration time was also similar (p > 0.05) for NSER versus LAP, respectively: 32.3 ± 3.3 versus 30.8 ± 3.9 minutes. In conclusion, the NSER method results proved to be similar to the LAP technique in small-sized Canindé goats. It was noticeable, however, that the NSER technique is simpler and provides the possibility for successive procedures with few health risks and sequels for females. This study may hopefully boost in vivo embryo production programs in the Canindé breed, facilitating the formation of embryo banks and so assuring the availability of genetic diversity before any decline becomes irreversible. MenosAbstract: By allowing for the creation of embryo banks, reproductive biotechnologies play an essential role in the preservation of endangered goat breeds' genetic diversity. This study focused on comparing both available embryo collection methods [laparotomy (LAP) vs. nonsurgical embryo recovery (NSER)] in Canindé goats to create an embryo bank for later use in a breed conservation program. Twelve females were superovulated and subjected to either the LAP or NSER technique for embryo recovery. The recovery rate was similar (p>0.05) between NSER (86.8% ± 5.6%) and LAP (92.8% ± 4.0%). Moreover, there were no differences (p > 0.05) in the number of structures recovered, the viable embryos, and the freezable embryos per goat, respectively, for NSER (11.7 ± 1.3, 11.2 ± 1.5, and 10.2 ± 1.1) and LAP (10.3 ± 1.0, 8.7 ± 0.7, and 8.0 ± 0.8). Overall, 132 structures were collected out of 151 ovulations ( 12.6 ± 1.2 corpora lutea per goat). Finally, the procedure duration time was also similar (p > 0.05) for NSER versus LAP, respectively: 32.3 ± 3.3 versus 30.8 ± 3.9 minutes. In conclusion, the NSER method results proved to be similar to the LAP technique in small-sized Canindé goats. It was noticeable, however, that the NSER technique is simpler and provides the possibility for successive procedures with few health risks and sequels for females. This study may hopefully boost in vivo embryo production programs in the Canindé breed, facilitating the formation of embryo banks and so assu... Mostrar Tudo |
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Palavras-Chave: |
Endangered breed; Germplasm bank; Nonsurgical embryo recovery; NSER. |
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Thesaurus Nal: |
Brazil; Caninde (goat breed); Conservation banking; Conservation programs; Goats; Laparotomy; Reproduction; Species diversity. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02693naa a2200349 a 4500
001 2134141
005 2022-05-10
008 2021 bl uuuu u00u1 u #d
024 7 $ahttp://doi.org/10.1089/bio.2021.0041$2DOI
100 1 $aSOUZA-FABJAN, J. M. G.
245 $aTranscervical versus laparotomy embryo recovery$bwhat strategy is best for embryo bank formation in the Caninde goat conservation program?$h[electronic resource]
260 $c2021
520 $aAbstract: By allowing for the creation of embryo banks, reproductive biotechnologies play an essential role in the preservation of endangered goat breeds' genetic diversity. This study focused on comparing both available embryo collection methods [laparotomy (LAP) vs. nonsurgical embryo recovery (NSER)] in Canindé goats to create an embryo bank for later use in a breed conservation program. Twelve females were superovulated and subjected to either the LAP or NSER technique for embryo recovery. The recovery rate was similar (p>0.05) between NSER (86.8% ± 5.6%) and LAP (92.8% ± 4.0%). Moreover, there were no differences (p > 0.05) in the number of structures recovered, the viable embryos, and the freezable embryos per goat, respectively, for NSER (11.7 ± 1.3, 11.2 ± 1.5, and 10.2 ± 1.1) and LAP (10.3 ± 1.0, 8.7 ± 0.7, and 8.0 ± 0.8). Overall, 132 structures were collected out of 151 ovulations ( 12.6 ± 1.2 corpora lutea per goat). Finally, the procedure duration time was also similar (p > 0.05) for NSER versus LAP, respectively: 32.3 ± 3.3 versus 30.8 ± 3.9 minutes. In conclusion, the NSER method results proved to be similar to the LAP technique in small-sized Canindé goats. It was noticeable, however, that the NSER technique is simpler and provides the possibility for successive procedures with few health risks and sequels for females. This study may hopefully boost in vivo embryo production programs in the Canindé breed, facilitating the formation of embryo banks and so assuring the availability of genetic diversity before any decline becomes irreversible.
650 $aBrazil
650 $aCaninde (goat breed)
650 $aConservation banking
650 $aConservation programs
650 $aGoats
650 $aLaparotomy
650 $aReproduction
650 $aSpecies diversity
653 $aEndangered breed
653 $aGermplasm bank
653 $aNonsurgical embryo recovery
653 $aNSER
700 1 $aBATISTA, R. I. T. P.
700 1 $aMELO, L. M.
700 1 $aOLIVEIRA, M. A.
700 1 $aCHAVES, M. S.
700 1 $aFONSECA, J. F. da
700 1 $aFREITAS, V. J. F.
773 $tBiopreservation and Biobanking$gv. 20, n. 2, p. 204-207, Sept. 2021.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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