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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com rosangela.lacerda@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
11/08/2021 |
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Data da última atualização: |
15/09/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ASSUNÇÃO, C. M.; MENDES, V. R. A.; BRANDAO, F. Z.; BATISTA, R. I. T. P.; SOUZA, E. D.; CARVALHO, B. C. de; QUINTAO, C. C. R.; RAPOSO, N. R. B.; CAMARGO, L. S. de A. |
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Afiliação: |
CAROLINA MARINHO ASSUNÇÃO, Universidade Federal de Juiz de Fora; VIVIAN RACHEL ARAUJO MENDES, Universidade Federal de Viçosa; FELIPE ZANDONADI BRANDÃO, Universidade Federal Fluminense; RIBRIO IVAN TAVARES PEREIRA BATISTA, Universidade Federal Fluminense; ELIZA DINIZ SOUZA; BRUNO CAMPOS DE CARVALHO, CNPGL; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; NADIA REZENDE BARBOSA RAPOSO, Universidade Federal de Juiz de Fora; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Effects of resveratrol in bull semen extender on post-thaw sperm quality and capacity for fertilization and embryo development. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Animal Reproduction Science, v. 226, 106697, 2021. |
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DOI: |
https://doi.org/10.1016/j.anireprosci.2021.106697 |
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Idioma: |
Inglês |
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Conteúdo: |
Resveratrol, a potent antioxidant, can be an alternative semen extender constituent to protect spermatozoa against reactive oxygen species (ROS); however, effects on sperm quality postthawing and sperm function is not well understood. This study, therefore, was conducted to investigate effects of resveratrol supplementation to semen extender on sperm quality postthawing. Bull semen was cryopreserved using extenders not supplemented or supplemented with 0.05, 0.1, or 1 mM resveratrol. Supplementation of extender with resveratrol at 0.05 mM resulted in greater (P < 0.05) sperm progressive motility, average path velocity, straight linear velocity, linearity and straightness when compared with no or 1 mM supplementations. Furthermore, effects of 0.05 mM resveratrol supplementations on plasma membrane and acrosome integrity and sperm fertilization capacity using in vitro procedures were investigated. Supplementation of semen extender with resveratrol resulted in a greater (P < 0.05) proportion of frozen-thawed spermatozoa with an intact acrosome and plasma membrane. Results from in vitro fertilization studies indicated there were no differences (P> 0.05) when there was no supplementation or supplementation with 0.05 mM resveratrol on embryo development to the cleavage and blastocyst stages. In conclusion, addition of resveratrol to bull semen extender resulted in greater sperm quality post-thawing in a dose-dependent manner, with values for variables related to sperm quality being greater when there was resveratrol supplementation at the 0.05 mM concentration. Proportion of embryo developing to the cleavage and blastocyst stages after in vitro fertilization was not affected by resveratrol supplementation to semen extenders. MenosResveratrol, a potent antioxidant, can be an alternative semen extender constituent to protect spermatozoa against reactive oxygen species (ROS); however, effects on sperm quality postthawing and sperm function is not well understood. This study, therefore, was conducted to investigate effects of resveratrol supplementation to semen extender on sperm quality postthawing. Bull semen was cryopreserved using extenders not supplemented or supplemented with 0.05, 0.1, or 1 mM resveratrol. Supplementation of extender with resveratrol at 0.05 mM resulted in greater (P < 0.05) sperm progressive motility, average path velocity, straight linear velocity, linearity and straightness when compared with no or 1 mM supplementations. Furthermore, effects of 0.05 mM resveratrol supplementations on plasma membrane and acrosome integrity and sperm fertilization capacity using in vitro procedures were investigated. Supplementation of semen extender with resveratrol resulted in a greater (P < 0.05) proportion of frozen-thawed spermatozoa with an intact acrosome and plasma membrane. Results from in vitro fertilization studies indicated there were no differences (P> 0.05) when there was no supplementation or supplementation with 0.05 mM resveratrol on embryo development to the cleavage and blastocyst stages. In conclusion, addition of resveratrol to bull semen extender resulted in greater sperm quality post-thawing in a dose-dependent manner, with values for variables related to sperm quality bein... Mostrar Tudo |
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Palavras-Chave: |
Desenvolvimento fetal; Embryo development; Fertilização in vitro. |
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Thesagro: |
Bovino; Criopreservação; Esperma; Reprodução Animal. |
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Thesaurus Nal: |
Antioxidants; Cryopreservation; In vitro fertilization. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02853naa a2200349 a 4500
001 2133482
005 2021-09-15
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.anireprosci.2021.106697$2DOI
100 1 $aASSUNÇÃO, C. M.
245 $aEffects of resveratrol in bull semen extender on post-thaw sperm quality and capacity for fertilization and embryo development.$h[electronic resource]
260 $c2021
520 $aResveratrol, a potent antioxidant, can be an alternative semen extender constituent to protect spermatozoa against reactive oxygen species (ROS); however, effects on sperm quality postthawing and sperm function is not well understood. This study, therefore, was conducted to investigate effects of resveratrol supplementation to semen extender on sperm quality postthawing. Bull semen was cryopreserved using extenders not supplemented or supplemented with 0.05, 0.1, or 1 mM resveratrol. Supplementation of extender with resveratrol at 0.05 mM resulted in greater (P < 0.05) sperm progressive motility, average path velocity, straight linear velocity, linearity and straightness when compared with no or 1 mM supplementations. Furthermore, effects of 0.05 mM resveratrol supplementations on plasma membrane and acrosome integrity and sperm fertilization capacity using in vitro procedures were investigated. Supplementation of semen extender with resveratrol resulted in a greater (P < 0.05) proportion of frozen-thawed spermatozoa with an intact acrosome and plasma membrane. Results from in vitro fertilization studies indicated there were no differences (P> 0.05) when there was no supplementation or supplementation with 0.05 mM resveratrol on embryo development to the cleavage and blastocyst stages. In conclusion, addition of resveratrol to bull semen extender resulted in greater sperm quality post-thawing in a dose-dependent manner, with values for variables related to sperm quality being greater when there was resveratrol supplementation at the 0.05 mM concentration. Proportion of embryo developing to the cleavage and blastocyst stages after in vitro fertilization was not affected by resveratrol supplementation to semen extenders.
650 $aAntioxidants
650 $aCryopreservation
650 $aIn vitro fertilization
650 $aBovino
650 $aCriopreservação
650 $aEsperma
650 $aReprodução Animal
653 $aDesenvolvimento fetal
653 $aEmbryo development
653 $aFertilização in vitro
700 1 $aMENDES, V. R. A.
700 1 $aBRANDAO, F. Z.
700 1 $aBATISTA, R. I. T. P.
700 1 $aSOUZA, E. D.
700 1 $aCARVALHO, B. C. de
700 1 $aQUINTAO, C. C. R.
700 1 $aRAPOSO, N. R. B.
700 1 $aCAMARGO, L. S. de A.
773 $tAnimal Reproduction Science$gv. 226, 106697, 2021.
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