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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
11/08/2021 |
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Data da última atualização: |
11/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GOGONE, I. C. V. P.; FERREIRA, G. H.; GAVA, D.; SCHAEFER, R.; PAULA-LOPES, F. F. de; ROCHA, R. de A.; BARROS, F. R. O. de. |
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Afiliação: |
IZABEL C. V. P. COGONE, UTFPR; GLAUCIA H. FERREIRA, UNIFESP/Diadema; DANIELLE GAVA, CNPSA; REJANE SCHAEFER, CNPSA; FABÍOLA F. DE PAULA-LOPES, UNIFESP/Diadema; RAQUEL DE A. ROCHA, UTFPR; FLAVIA REGINA OLIVEIRA DE BARROS, UTFPR. |
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Título: |
Applicability of Raman spectroscopy on porcine parvovirus and porcine circovirus type 2 detection. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, v. 249, n. 119336, 2021. |
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DOI: |
https://doi.org/10.1016/j.saa.2020.119336 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Porcine parvovirus (PPV) is one of the major infectious causes of reproductive failure of swine. This disease is characterized by embryonic and fetal infection and death, responsible for important economic losses. PPV is also implicated as a trigger in the development of post-weaning multisystemic wasting syndrome (PMWS) caused by Porcine circovirus type 2 (PCV2). Their detection is PCR-based, which is quite sensitive and specific, but laborious, costly and time-demanding. Therefore, this study aimed to assess Raman spectroscopy (RS) as a diagnostic tool for PPV and PCV2 due to its label-free properties and unique ability to search and identify molecular fingerprints. Briefly, swine testis (ST) cells were inoculated with PPV or PCV2 and in vitro cultured (37 C, 5% CO2) for four days. Fixed cells were then submitted to RS investigation using a 633 nm laser. A total of 225 spectra centered at 1300 cm1 was obtained for each sample (5 spectra/cell; 15 cells/replicate; 3 replicates) of PPV-, PCV2-infected and uninfected (control) ST cells. Clear statistical discrimination between samples from both virus-infected cells was achieved with a Principal Component ? Linear Discriminant Analysis (PCA-LDA) model, reaching sensitivity rates from 95.55% to 97.77%, respectively to PCV2- and PPV-infected cells. These results were then submitted to a Leave-One-Out (LOO) validation algorithm resulting in 99.97% of accuracy. Extensive band assignment was analyzed and compiled for better understanding of PPV and PCV2 virus-cell interaction, demonstrating that specific protein, lipids and DNA/RNA bands are the most important assignments related to discrimination of virus-infected from uninfected cells. In conclusion, these results represent promising bases for RS application on PCV2 and PPV detection for future diagnostic applications. MenosAbstract: Porcine parvovirus (PPV) is one of the major infectious causes of reproductive failure of swine. This disease is characterized by embryonic and fetal infection and death, responsible for important economic losses. PPV is also implicated as a trigger in the development of post-weaning multisystemic wasting syndrome (PMWS) caused by Porcine circovirus type 2 (PCV2). Their detection is PCR-based, which is quite sensitive and specific, but laborious, costly and time-demanding. Therefore, this study aimed to assess Raman spectroscopy (RS) as a diagnostic tool for PPV and PCV2 due to its label-free properties and unique ability to search and identify molecular fingerprints. Briefly, swine testis (ST) cells were inoculated with PPV or PCV2 and in vitro cultured (37 C, 5% CO2) for four days. Fixed cells were then submitted to RS investigation using a 633 nm laser. A total of 225 spectra centered at 1300 cm1 was obtained for each sample (5 spectra/cell; 15 cells/replicate; 3 replicates) of PPV-, PCV2-infected and uninfected (control) ST cells. Clear statistical discrimination between samples from both virus-infected cells was achieved with a Principal Component ? Linear Discriminant Analysis (PCA-LDA) model, reaching sensitivity rates from 95.55% to 97.77%, respectively to PCV2- and PPV-infected cells. These results were then submitted to a Leave-One-Out (LOO) validation algorithm resulting in 99.97% of accuracy. Extensive band assignment was analyzed and compiled for bette... Mostrar Tudo |
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Palavras-Chave: |
Molecular fingerprint; PCV2; PPV; Reproductive failure; Viral diagnostic. |
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Thesagro: |
Diagnostico; Parvovirose; Sanidade Animal; Suíno. |
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Thesaurus Nal: |
Animal diseases; Porcine circovirus-2; Raman spectroscopy; Reproductive success; Swine. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02976naa a2200373 a 4500
001 2133480
005 2021-08-11
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.saa.2020.119336$2DOI
100 1 $aGOGONE, I. C. V. P.
245 $aApplicability of Raman spectroscopy on porcine parvovirus and porcine circovirus type 2 detection.$h[electronic resource]
260 $c2021
520 $aAbstract: Porcine parvovirus (PPV) is one of the major infectious causes of reproductive failure of swine. This disease is characterized by embryonic and fetal infection and death, responsible for important economic losses. PPV is also implicated as a trigger in the development of post-weaning multisystemic wasting syndrome (PMWS) caused by Porcine circovirus type 2 (PCV2). Their detection is PCR-based, which is quite sensitive and specific, but laborious, costly and time-demanding. Therefore, this study aimed to assess Raman spectroscopy (RS) as a diagnostic tool for PPV and PCV2 due to its label-free properties and unique ability to search and identify molecular fingerprints. Briefly, swine testis (ST) cells were inoculated with PPV or PCV2 and in vitro cultured (37 C, 5% CO2) for four days. Fixed cells were then submitted to RS investigation using a 633 nm laser. A total of 225 spectra centered at 1300 cm1 was obtained for each sample (5 spectra/cell; 15 cells/replicate; 3 replicates) of PPV-, PCV2-infected and uninfected (control) ST cells. Clear statistical discrimination between samples from both virus-infected cells was achieved with a Principal Component ? Linear Discriminant Analysis (PCA-LDA) model, reaching sensitivity rates from 95.55% to 97.77%, respectively to PCV2- and PPV-infected cells. These results were then submitted to a Leave-One-Out (LOO) validation algorithm resulting in 99.97% of accuracy. Extensive band assignment was analyzed and compiled for better understanding of PPV and PCV2 virus-cell interaction, demonstrating that specific protein, lipids and DNA/RNA bands are the most important assignments related to discrimination of virus-infected from uninfected cells. In conclusion, these results represent promising bases for RS application on PCV2 and PPV detection for future diagnostic applications.
650 $aAnimal diseases
650 $aPorcine circovirus-2
650 $aRaman spectroscopy
650 $aReproductive success
650 $aSwine
650 $aDiagnostico
650 $aParvovirose
650 $aSanidade Animal
650 $aSuíno
653 $aMolecular fingerprint
653 $aPCV2
653 $aPPV
653 $aReproductive failure
653 $aViral diagnostic
700 1 $aFERREIRA, G. H.
700 1 $aGAVA, D.
700 1 $aSCHAEFER, R.
700 1 $aPAULA-LOPES, F. F. de
700 1 $aROCHA, R. de A.
700 1 $aBARROS, F. R. O. de
773 $tSpectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy$gv. 249, n. 119336, 2021.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Biblioteca |
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Tipo/Formato |
Classificação |
Cutter |
Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria Tropical. |
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Data corrente: |
12/02/2001 |
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Data da última atualização: |
19/04/2010 |
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Autoria: |
CAVALCANTI, J. J. V.; CARDOSO, J. E.; BARROS, L. de M.; FELIPE, E. M. |
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Título: |
Resistência genética de clones de cajueiro anão precoce às principais fitomoléstias. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Fortaleza: Embrapa Agroindústria Tropical, 2000. |
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Páginas: |
15 p. |
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Série: |
(Embrapa Agroindústria Tropical. Boletim de Pesquisa, 34). |
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Idioma: |
Português |
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Conteúdo: |
No processo seletivo de clones de cajueiro anão precoce, diversos atributos têm sido considerados. Entretanto, o uso da resistência genética a doenças ainda e pouco conhecida e explorada. Este trabalho se propos determinar a variabilidade genética e identificar genótipos resistentes de cajueiro anão precoce quanto a antracnose (Colletotrichum gloeosporioides), ao mofo preto (Pilgeriella anacardium) e a mancha-angular (Septoria anacardil), as doenças mais importantes da cultura no Brasil. Os coeficientes de determinação genotípica foram de 80,72%, 85,20% e 74,92% para a antracnose, mofo-preto e mancha-angular, respectivamente. Os clones que demonstraram maior grau de resistência foram CAP 14, CAP 17, CAP 05 e CAP 07, para a antracnose; CAP 08, CAP 17 e CAP 11, para o mofo-preto e CAP 02, CAP 05 e CAP 03, para a mancha-angular. Verificou-se que entre os clones comerciais, o CCP 06 apresentou maior resistência a antracnose e ao mofo-preto, enquanto que os clones CCP 76 e CCP 1001 foram mais suscetíveis ao mofo-preto e o CCP 09 a antracnose. Os resultados indicam ampla variabilidade genética entre os clones, para todas as doenças avaliadas, possibilitando progresso genético por meio de seleção fenotípica. |
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Palavras-Chave: |
Angular leaf spot; Anthracnosis; AntracnoseM Morfo Preto; Black mould; Brasil; Breedings; Caju anao; Cajueiro; Cajueiro anao precoce; Cashew; Cashews: Tropical fruits; Ceara; Disease; Diseases; Fortaleza; Mancha-angular; Melhoramento gen?tico; Melhoramento Genetico; Mofo-preto; Pilgeriella; Pilgeriella anacardium; Plant breding; Plant diseases; Resistance; Resistencia a Doencas; Septoria anacardii; Septoria anacardium Dwarf cashews. |
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Thesagro: |
Anacardium Occidentale; Antracnose; Caju; Clone; Colletotrichum Gloeosporioides; Doença; Doença de Planta; Fruta Tropical; Fungo; Genótipo; Mancha Angular; Melhoramento Genético Vegetal; Mofo Preto; Resistência; Variedade Resistente. |
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Thesaurus NAL: |
Anacardium; anthracnose; clones; disease resistance; fungi; genotype; plant breeding. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CNPAT-2010/5486/1/Bp-034.pdf
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Marc: |
LEADER 03277nam a2200757 a 4500
001 1422725
005 2010-04-19
008 2000 bl uuuu u0uu1 u #d
100 1 $aCAVALCANTI, J. J. V.
245 $aResistência genética de clones de cajueiro anão precoce às principais fitomoléstias.
260 $aFortaleza: Embrapa Agroindústria Tropical$c2000
300 $a15 p.
490 $a(Embrapa Agroindústria Tropical. Boletim de Pesquisa, 34).
520 $aNo processo seletivo de clones de cajueiro anão precoce, diversos atributos têm sido considerados. Entretanto, o uso da resistência genética a doenças ainda e pouco conhecida e explorada. Este trabalho se propos determinar a variabilidade genética e identificar genótipos resistentes de cajueiro anão precoce quanto a antracnose (Colletotrichum gloeosporioides), ao mofo preto (Pilgeriella anacardium) e a mancha-angular (Septoria anacardil), as doenças mais importantes da cultura no Brasil. Os coeficientes de determinação genotípica foram de 80,72%, 85,20% e 74,92% para a antracnose, mofo-preto e mancha-angular, respectivamente. Os clones que demonstraram maior grau de resistência foram CAP 14, CAP 17, CAP 05 e CAP 07, para a antracnose; CAP 08, CAP 17 e CAP 11, para o mofo-preto e CAP 02, CAP 05 e CAP 03, para a mancha-angular. Verificou-se que entre os clones comerciais, o CCP 06 apresentou maior resistência a antracnose e ao mofo-preto, enquanto que os clones CCP 76 e CCP 1001 foram mais suscetíveis ao mofo-preto e o CCP 09 a antracnose. Os resultados indicam ampla variabilidade genética entre os clones, para todas as doenças avaliadas, possibilitando progresso genético por meio de seleção fenotípica.
650 $aAnacardium
650 $aanthracnose
650 $aclones
650 $adisease resistance
650 $afungi
650 $agenotype
650 $aplant breeding
650 $aAnacardium Occidentale
650 $aAntracnose
650 $aCaju
650 $aClone
650 $aColletotrichum Gloeosporioides
650 $aDoença
650 $aDoença de Planta
650 $aFruta Tropical
650 $aFungo
650 $aGenótipo
650 $aMancha Angular
650 $aMelhoramento Genético Vegetal
650 $aMofo Preto
650 $aResistência
650 $aVariedade Resistente
653 $aAngular leaf spot
653 $aAnthracnosis
653 $aAntracnoseM Morfo Preto
653 $aBlack mould
653 $aBrasil
653 $aBreedings
653 $aCaju anao
653 $aCajueiro
653 $aCajueiro anao precoce
653 $aCashew
653 $aCashews: Tropical fruits
653 $aCeara
653 $aDisease
653 $aDiseases
653 $aFortaleza
653 $aMancha-angular
653 $aMelhoramento gen?tico
653 $aMelhoramento Genetico
653 $aMofo-preto
653 $aPilgeriella
653 $aPilgeriella anacardium
653 $aPlant breding
653 $aPlant diseases
653 $aResistance
653 $aResistencia a Doencas
653 $aSeptoria anacardii
653 $aSeptoria anacardium Dwarf cashews
700 1 $aCARDOSO, J. E.
700 1 $aBARROS, L. de M.
700 1 $aFELIPE, E. M.
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Embrapa Agroindústria Tropical (CNPAT) |
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