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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com rosangela.lacerda@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
11/08/2021 |
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Data da última atualização: |
11/08/2021 |
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Tipo da produção científica: |
Nota Técnica/Nota Científica |
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Autoria: |
FONSECA, M. D. M.; MAIA, J. M. S.; VARAGO, F. C.; GERN, J. C.; CARVALHO, W. A.; SILVA, S. R.; MOSQUEIRA, V. C. F.; BRANDAO, H. de M.; GUIMARÃES, A. S. |
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Afiliação: |
Universidade Federal de Lavras; Universidade Federal de Lavras; JULIANA CARINE GERN, CNPGL; WANESSA ARAUJO CARVALHO, CNPGL; Universidade Federal de Ouro Preto; Universidade Federal de Ouro Preto; HUMBERTO DE MELLO BRANDAO, CNPGL; ALESSANDRO DE SA GUIMARAES, CNPGL. |
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Título: |
Cloxacillin nanostructured formulation for the treatment of bovine keratoconjunctivitis. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Veterinary and Animal Science, v. 9, 100089, 2020. |
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DOI: |
https://doi.org/10.1016/j.vas.2020.100089 |
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Idioma: |
Inglês |
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Notas: |
Short communication. |
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Conteúdo: |
Infectious bovine keratoconjunctivitis (IBK) is a widespread, contagious ocular disease that affects cattle, especially dairy breeds. The disease is caused by Gram-negative bacteria mainly Moraxella bovis, and its treatment consists of parenteral or topic antibiotic therapy. The topic treatment approach is used more commonly in lactating cows, to avoid milk disposal. However, treatment failures are common, because the antibiotic is removed during lacrimation. This study aimed to evaluate the susceptibility of commercial cloxacillin and evaluate the efficacy of nanostructured cloxacillin in clinical cases of IBK by Moraxella. The minimum inhibitory concentration (MIC) of nanoparticle cloxacillin nanocoated, the nanoparticle without the antibiotic and the commercial cloxacillin were determined in vitro with field samples of Moraxella ovis (5) and Moraxella bovis (5). The efficiency of nanoparticles was tested in three cows naturally infected that were treated with 1.0 mL (with 0.32 mg of nanostructured cloxacillin) for the ocular route. Moraxella bovis was isolated and identified by biochemical and molecular methods before the treatment. The animals were treated every 12 h for six days. The cure was considered by the absence of clinical symptoms and bacteria after treatment. The mucoadhesive nanoparticlebased formulation promoted clinical cure with a low number of doses of antibiotics, probably due to the maintenance of the MIC in the ocular mucosa for longer due to the mucoadhesive characteristics of the nanoparticle. The results indicate that the use of nanocoated cloxacillin is possible to control infectious bovine keratoconjunctivitis. MenosInfectious bovine keratoconjunctivitis (IBK) is a widespread, contagious ocular disease that affects cattle, especially dairy breeds. The disease is caused by Gram-negative bacteria mainly Moraxella bovis, and its treatment consists of parenteral or topic antibiotic therapy. The topic treatment approach is used more commonly in lactating cows, to avoid milk disposal. However, treatment failures are common, because the antibiotic is removed during lacrimation. This study aimed to evaluate the susceptibility of commercial cloxacillin and evaluate the efficacy of nanostructured cloxacillin in clinical cases of IBK by Moraxella. The minimum inhibitory concentration (MIC) of nanoparticle cloxacillin nanocoated, the nanoparticle without the antibiotic and the commercial cloxacillin were determined in vitro with field samples of Moraxella ovis (5) and Moraxella bovis (5). The efficiency of nanoparticles was tested in three cows naturally infected that were treated with 1.0 mL (with 0.32 mg of nanostructured cloxacillin) for the ocular route. Moraxella bovis was isolated and identified by biochemical and molecular methods before the treatment. The animals were treated every 12 h for six days. The cure was considered by the absence of clinical symptoms and bacteria after treatment. The mucoadhesive nanoparticlebased formulation promoted clinical cure with a low number of doses of antibiotics, probably due to the maintenance of the MIC in the ocular mucosa for longer due to the mucoad... Mostrar Tudo |
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Palavras-Chave: |
Ceratoconjuntivite; Cloxacilina; Nanoestrutura; Nanopartícula mucoadesiva. |
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Thesagro: |
Bovino; Doença Animal; Moraxella Bovis. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02630naa a2200325 a 4500
001 2133453
005 2021-08-11
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.vas.2020.100089$2DOI
100 1 $aFONSECA, M. D. M.
245 $aCloxacillin nanostructured formulation for the treatment of bovine keratoconjunctivitis.$h[electronic resource]
260 $c2020
500 $aShort communication.
520 $aInfectious bovine keratoconjunctivitis (IBK) is a widespread, contagious ocular disease that affects cattle, especially dairy breeds. The disease is caused by Gram-negative bacteria mainly Moraxella bovis, and its treatment consists of parenteral or topic antibiotic therapy. The topic treatment approach is used more commonly in lactating cows, to avoid milk disposal. However, treatment failures are common, because the antibiotic is removed during lacrimation. This study aimed to evaluate the susceptibility of commercial cloxacillin and evaluate the efficacy of nanostructured cloxacillin in clinical cases of IBK by Moraxella. The minimum inhibitory concentration (MIC) of nanoparticle cloxacillin nanocoated, the nanoparticle without the antibiotic and the commercial cloxacillin were determined in vitro with field samples of Moraxella ovis (5) and Moraxella bovis (5). The efficiency of nanoparticles was tested in three cows naturally infected that were treated with 1.0 mL (with 0.32 mg of nanostructured cloxacillin) for the ocular route. Moraxella bovis was isolated and identified by biochemical and molecular methods before the treatment. The animals were treated every 12 h for six days. The cure was considered by the absence of clinical symptoms and bacteria after treatment. The mucoadhesive nanoparticlebased formulation promoted clinical cure with a low number of doses of antibiotics, probably due to the maintenance of the MIC in the ocular mucosa for longer due to the mucoadhesive characteristics of the nanoparticle. The results indicate that the use of nanocoated cloxacillin is possible to control infectious bovine keratoconjunctivitis.
650 $aBovino
650 $aDoença Animal
650 $aMoraxella Bovis
653 $aCeratoconjuntivite
653 $aCloxacilina
653 $aNanoestrutura
653 $aNanopartícula mucoadesiva
700 1 $aMAIA, J. M. S.
700 1 $aVARAGO, F. C.
700 1 $aGERN, J. C.
700 1 $aCARVALHO, W. A.
700 1 $aSILVA, S. R.
700 1 $aMOSQUEIRA, V. C. F.
700 1 $aBRANDAO, H. de M.
700 1 $aGUIMARÃES, A. S.
773 $tVeterinary and Animal Science$gv. 9, 100089, 2020.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 35 | |
| 3. |  | ROSA, A. J. de M.; FRAGOSO, R. da R. Análise genômica em bovinos. Grupo Cultivar, 30 mar. 2010. Publicado também em: Agronline, 31 mar. 2010; Campo e Criação, 31 mar. 2010; Araucária Genética Bovina, 01 abr. 2010; AgroRede Notícias, 01 abr. 2010; ZooNews, 05 abr. 2010; Portal Dia de Campo, 08 abr. 2010| Tipo: Artigo de Divulgação na Mídia |
| Biblioteca(s): Embrapa Cerrados. |
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| 4. | | ROSA, A. J. de M.; MOREIRA, J. R. Mecanismos de evolução. In: MOREIRA, J. R.; MEDEIROS, M. B. de (Ed.). O legado de Darwin e a pesquisa agropecuária. Brasília, DF: Embrapa, 2014. p. 93-132 Unidade responsável pelo conteúdo: Embrapa Recursos Genéticos e Biotecnologia.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | FUKUSHIMA, R. S.; ROSA, A. J. de M.; LIMA, C. G. de; CUNHA, J. A. da. Comparação entre dois métodos analíticos para determinação da lignina de algumas gramíneas forrageiras. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 34, n. 6, p. 1025-30, jun. 1999 Título em inglês: Comparison between two analytical methods for determining lignin concentration of some grass forages.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 7. | | FRAGOSO, R. da R.; NAKASU, E. Y. T.; ROCHA, T. L.; ROSA, A. J. de M. Genômica funcional. In: FALEIRO, F. G.; ANDRADE, S. R. M. de; REIS JUNIOR, F. B. dos. (Ed.). Biotecnologia: estado da arte e aplicações na agropecuária. Planaltina, DF: Embrapa Cerrados, 2011. 729 p. il. Color. p. 143-173| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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| 13. | | ROSA, A. J. de M.; SARDINA, M. T.; MASTRANGELO, S.; TOLONE, M.; PORTOLANO, B. Parentage verification of Valle del Belice dairy sheep using multiplex microsatellite panel. Small Ruminant Research, v. 113, n. 1, p. 62-65, June, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Cerrados. |
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| 14. | | GEORGE, S.; CIRCLE, K.; LINDBLOM, S.; VILAIN, S.; ROSA, A. J. de M.; FRANCIS, D.; BRÖZEL, V.; KAUSHIK, R. S. Assessment of toll-like receptors in the ileum of weanling pigs - responses to feed antibiotic chlortetracycline and gnotobiotic conditions. Journal of Clinical and Cellular Immunology, v. 3, issue 3, 2012. 8 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Cerrados. |
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| 15. | | REGITANO, L. C. de A.; AZEVEDO, J. L.; ROSA, A. J. M.; PACKER, I. U.; COUTINHO, L. L. Evidence of selection for growth hormone, microsatellites IGF-I and CSFM50 in Canchim bovine breed. Revista Brasileira de Genética, v.20, n.3, suppl., 1997, I.131, p.334.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 17. | | ROSA, A. J. M.; REGITANO, L. C. de A.; MERZEL, M.; PACKER, I. U.; RAZOOK, A. G.; COUTINHO, L. L. Polymorphism of growth hormone, microsatellite IGF-I and association with feedlot performance in Nelore cattle. Revista Brasileira de Genética, Ribeirao Preto, v.19, n.3 supl, p.297, 1996. Resumo.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 18. | | LIMA, M. F. S. G.; JESUS, F. G. de; ALMEIDA, A. C. de S.; NASCIMENTO, W. M.; ROSA, A. J. Nível de controle de Chloridea virescens (Lepidoptera: Noctuidae ) em grão-de-bico. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 29.; CONGRESSO LATINO-AMERICANO, 13., 2024, Uberlândia. Anais... [S.l.]: Sociedade Entomológica do Brasil, 2024. p. 1264| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| 19. | | RETTEDAL, E.; VILAIN, S.; LINDBLOM, S.; LEHNERT, K.; SCOFIELD, C.; GEORGE, S.; CLAY, S.; KAUSHIK, R. S.; ROSA, A. J. de M.; FRANCIS, D.; BRÖZEL, V. S. Alteration of the ileal microbiota of weanling piglets by the growth-promoting antibiotic chlortetracycline. Applied and Environmental Microbiology, v. 75, n. 17, p. 5489-5495, Sept. 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Cerrados. |
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| 20. | | DUARTE, I. de A. E.; MILENKOVIC, D.; BORGES, T. K. dos S.; ROSA, A. J. de M.; MORAND, C.; OLIVEIRA, L. de L. de; COSTA, A. M. Acute Effects of the Consumption of Passiflora setacea Juice on Metabolic Risk Factors and Gene Expression Profile in Humans. Nutrients, v. 12, n. 1104, 2020. 20 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Cerrados. |
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| Registros recuperados : 35 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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