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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
30/06/2021 |
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Data da última atualização: |
30/06/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, C. M. dos; RIBEIRO, N. V. S.; SCHWAB, S.; BALDANI, J. I.; VIDAL, M. S. |
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Afiliação: |
CARLOS M. DOS SANTOS, Bolsista da Embrapa Agrobiologia; NÁTHALIA V. S. RIBEIRO, Bolsista da Embrapa Agrobiologia; STEFAN SCHWAB, CNPAB; JOSE IVO BALDANI, CNPAB; MARCIA SOARES VIDAL, CNPAB. |
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Título: |
The effect of inoculation of a diazotrophic bacterial consortium on the indigenous bacterial community structure of sugarcane apoplast fluid. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Current Microbiologym, Published online, 25 june 2021. |
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ISSN: |
1432-0991 |
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DOI: |
https://doi.org/10.1007/s00284-021-02571-0 |
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Idioma: |
Inglês |
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Conteúdo: |
The extracellular space in plants, termed the apoplast, has a pH and sugar content that enables bacterial growth and represents a possible niche for the establishment of endophytic bacteria. Previous studies have investigated the effects of diazotrophic bacteria inoculation in sugarcane varieties, but it has not yet been analyzed how the microbial community of apoplast fluid of sugarcane is affected. High-throughput next generation sequencing of the 16S rRNA gene was used throughout this study to determine the effect of inoculation with a diazotrophic bacteria consortium, previously isolated from sugarcane, on the native bacterial population of sugarcane variety RB867515 grown in the field. The analyses were carried out 450 days after inoculation. The results revealed the presence of 22 phyla, with predominance of Proteobacteria phylum. It was observed that the inoculated consortium changed the indigenous bacterial community structure of sugarcane apoplast fluid by decreasing diversity and evenness, interfering in the composition of rare species. Microbial community composition analysis revealed differences between treatments. The differential abundance test showed there were 43 amplicon sequence variants (ASVs) which were relatively more abundant in the inoculated treatment, with predominance of the Sphingomonas genus. The pre-dicted functions of the most abundant ASVs revealed the presence of genera related to plant growth promotion and protection against phytopathogens. Analysis to evaluate the occurrence of inoculated strains in the recovered data was not conclusive since the ASVs taxonomically close to the inoculated bacteria were observed in low abundance. The present study is the first report to elucidate the bacterial community in sugarcane apoplast fluid using a culture-independent approach. It demonstrated that the diazotrophic bacterial consortium interferes in the natural bacterial community in sugarcane variety RB867515 MenosThe extracellular space in plants, termed the apoplast, has a pH and sugar content that enables bacterial growth and represents a possible niche for the establishment of endophytic bacteria. Previous studies have investigated the effects of diazotrophic bacteria inoculation in sugarcane varieties, but it has not yet been analyzed how the microbial community of apoplast fluid of sugarcane is affected. High-throughput next generation sequencing of the 16S rRNA gene was used throughout this study to determine the effect of inoculation with a diazotrophic bacteria consortium, previously isolated from sugarcane, on the native bacterial population of sugarcane variety RB867515 grown in the field. The analyses were carried out 450 days after inoculation. The results revealed the presence of 22 phyla, with predominance of Proteobacteria phylum. It was observed that the inoculated consortium changed the indigenous bacterial community structure of sugarcane apoplast fluid by decreasing diversity and evenness, interfering in the composition of rare species. Microbial community composition analysis revealed differences between treatments. The differential abundance test showed there were 43 amplicon sequence variants (ASVs) which were relatively more abundant in the inoculated treatment, with predominance of the Sphingomonas genus. The pre-dicted functions of the most abundant ASVs revealed the presence of genera related to plant growth promotion and protection against phytopathogens. A... Mostrar Tudo |
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Palavras-Chave: |
Endophytic bacteria; Plant growth promotion bacteria. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02707naa a2200217 a 4500
001 2132685
005 2021-06-30
008 2021 bl uuuu u00u1 u #d
022 $a1432-0991
024 7 $ahttps://doi.org/10.1007/s00284-021-02571-0$2DOI
100 1 $aSANTOS, C. M. dos
245 $aThe effect of inoculation of a diazotrophic bacterial consortium on the indigenous bacterial community structure of sugarcane apoplast fluid.$h[electronic resource]
260 $c2021
520 $aThe extracellular space in plants, termed the apoplast, has a pH and sugar content that enables bacterial growth and represents a possible niche for the establishment of endophytic bacteria. Previous studies have investigated the effects of diazotrophic bacteria inoculation in sugarcane varieties, but it has not yet been analyzed how the microbial community of apoplast fluid of sugarcane is affected. High-throughput next generation sequencing of the 16S rRNA gene was used throughout this study to determine the effect of inoculation with a diazotrophic bacteria consortium, previously isolated from sugarcane, on the native bacterial population of sugarcane variety RB867515 grown in the field. The analyses were carried out 450 days after inoculation. The results revealed the presence of 22 phyla, with predominance of Proteobacteria phylum. It was observed that the inoculated consortium changed the indigenous bacterial community structure of sugarcane apoplast fluid by decreasing diversity and evenness, interfering in the composition of rare species. Microbial community composition analysis revealed differences between treatments. The differential abundance test showed there were 43 amplicon sequence variants (ASVs) which were relatively more abundant in the inoculated treatment, with predominance of the Sphingomonas genus. The pre-dicted functions of the most abundant ASVs revealed the presence of genera related to plant growth promotion and protection against phytopathogens. Analysis to evaluate the occurrence of inoculated strains in the recovered data was not conclusive since the ASVs taxonomically close to the inoculated bacteria were observed in low abundance. The present study is the first report to elucidate the bacterial community in sugarcane apoplast fluid using a culture-independent approach. It demonstrated that the diazotrophic bacterial consortium interferes in the natural bacterial community in sugarcane variety RB867515
653 $aEndophytic bacteria
653 $aPlant growth promotion bacteria
700 1 $aRIBEIRO, N. V. S.
700 1 $aSCHWAB, S.
700 1 $aBALDANI, J. I.
700 1 $aVIDAL, M. S.
773 $tCurrent Microbiologym, Published online, 25 june 2021.
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| 16. |  | CRUVINEL, P. E. MTACCON versao 2.0: uma ferramenta para controle e aquisição de dados do minitomógrafo de raios-X e raios-gama para aplicações multidisciplinares. In: REUNIÃO ANUAL DA SBPC, 41., jul. 1989, Fortaleza, CE. Ciência e Cultura, São Paulo, v.41, n.7, p.76, jul. 1989. Suplemento. Resumos. ref.17-A.8.1.| Biblioteca(s): Embrapa Instrumentação. |
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| 20. | | CRUVINEL, P. E. Rede colaborativa para auxílio ao desenvolvimento da agricultura no Brasil. In: FURLANI, C. E. A.; SILVA, R. P. da; ROSALÉN, D. L.; SILVA, J. G. F. da; REIS, E. F. dos; VENTURIM, J. B. (Ed.). A engenharia agrícola e o desenvolvimento das propriedades familiares. Vitória, ES: [s. n.], 2010. 1 CD-ROM. Originalmente apresentado no CONGRESO LATINOAMERICANO Y DEL CARIBE DE INGENIERIA AGRICOLA - CLIA, 9.; CONGRESSO BRASILEIRO DE ENGENHARIA AGRÍCOLA - CONBEA, 39., 2010, Vitória, ES.| Tipo: Capítulo em Livro Técnico-Científico |
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