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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
24/04/2021 |
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Data da última atualização: |
12/07/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TÁVORA, F. T. P. K.; BEVITORI, R.; MELLO, R. N. de; CINTRA, M. M. D. F.; OLIVEIRA NETO, O. B.; FONTES, W.; CASTRO, M. S.; SOUSA, M. V.; FRANCO, O. L.; REIS, A. M. dos. |
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Afiliação: |
FABIANO T. P. K. TÁVORA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA, MG; ROSANGELA BEVITORI, CNPAF; RAQUEL NEVES DE MELLO, CNPAF; MARIA MONICA DOMINGUES F CINTRA, CNPAF; OSMUNDO B. OLIVEIRA NETO, CENTRO UNIVERSITARIO UNIEURO, Brasília-DF; WAGNER FONTES, UNIVERSIDADE DE BRASÍLIA; MARIANA S. CASTRO, UNIVERSIDADE DE BRASÍLIA; MARCELO V. SOUSA, UNIVERSIDADE DE BRASÍLIA; OCTAVIO L. FRANCO, UNIVERSIDADE CATÓLICA DE BRASÍLIA; ANGELA MEHTA DOS REIS, Cenargen. |
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Título: |
Shotgun proteomics coupled to transient-inducible gene silencing reveal rice susceptibility genes as new sources for blast disease resistance. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Journal of Proteomics, v. 241, 104223, June 2021. |
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ISSN: |
1874-3919 |
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DOI: |
https://doi.org/10.1016/j.jprot.2021.104223 |
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Idioma: |
Inglês
Português |
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Notas: |
Na publicação: Angela Mehta. |
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Conteúdo: |
A comparative proteomic analysis between two near-isogenic rice lines, displaying a resistant and susceptible phenotype upon infection with Magnaporthe oryzae was performed. We identified and validated factors associ-ated with rice disease susceptibility, representing a flourishing source toward a more resolute rice-blast resis-tance. Proteome profiles were remarkably different during early infection (12 h post-inoculation), revealing several proteins with increased abundance in the compatible interaction. Potential players of rice susceptibility were selected and gene expression was evaluated by RT-qPCR. Gene Ontology analysis disclosed susceptibility gene-encoded proteins claimed to be involved in fungus sustenance and suppression of plant immunity, such as sucrose synthase 4-like, serpin-ZXA-like, nudix hydrolase15, and DjA2 chaperone protein. Two other candidate genes, picked from a previous transcriptome study, were added into our downstream analysis including pyr-abactin resistant-like 5 (OsPYL5), and rice ethylene-responsive factor 104 (OsERF104). Further, we validated their role in susceptibility by Transient-Induced Gene Silencing (TIGS) using short antisense oligodeoxyr-ibonucleotides that resulted in a remarkable reduction of foliar disease symptoms in the compatible interaction. Therefore, we successfully employed shotgun proteomics and antisense-based gene silencing to prospect and functionally validate rice potential susceptibility factors, which could be further explored to build rice-blast resistance. Significance: R gene-mediated disease resistance is race-specific and often not durable in the field. More recently, advancements in new breeding techniques (NBTs) have made plant disease susceptibility genes (S-genes) a new target to build a broad spectrum and more durable resistance, hence an alternative source to R-genes in breeding programs. We successfully coupled shotgun proteomics and gene silencing tools to prospect and validate new rice-bast susceptibility genes that can be further exploited toward a more resolute blast disease resistance. MenosA comparative proteomic analysis between two near-isogenic rice lines, displaying a resistant and susceptible phenotype upon infection with Magnaporthe oryzae was performed. We identified and validated factors associ-ated with rice disease susceptibility, representing a flourishing source toward a more resolute rice-blast resis-tance. Proteome profiles were remarkably different during early infection (12 h post-inoculation), revealing several proteins with increased abundance in the compatible interaction. Potential players of rice susceptibility were selected and gene expression was evaluated by RT-qPCR. Gene Ontology analysis disclosed susceptibility gene-encoded proteins claimed to be involved in fungus sustenance and suppression of plant immunity, such as sucrose synthase 4-like, serpin-ZXA-like, nudix hydrolase15, and DjA2 chaperone protein. Two other candidate genes, picked from a previous transcriptome study, were added into our downstream analysis including pyr-abactin resistant-like 5 (OsPYL5), and rice ethylene-responsive factor 104 (OsERF104). Further, we validated their role in susceptibility by Transient-Induced Gene Silencing (TIGS) using short antisense oligodeoxyr-ibonucleotides that resulted in a remarkable reduction of foliar disease symptoms in the compatible interaction. Therefore, we successfully employed shotgun proteomics and antisense-based gene silencing to prospect and functionally validate rice potential susceptibility factors, which could be furth... Mostrar Tudo |
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Palavras-Chave: |
Gene ontology; Plant immunity; PTO-based TIGS; RT-qPCR; S-gene; Transient-Induced gene silencing. |
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Thesagro: |
Arroz; Brusone; Doença de Planta; Oryza Sativa. |
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Thesaurus Nal: |
blast disease; disease resistance; foliar diseases; Magnaporthe oryzae; plant diseases and disorders; proteomics; rice. |
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Categoria do assunto: |
--
S Ciências Biológicas |
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Marc: |
LEADER 03484naa a2200469 a 4500
001 2131497
005 2021-07-12
008 2021 bl uuuu u00u1 u #d
022 $a1874-3919
024 7 $ahttps://doi.org/10.1016/j.jprot.2021.104223$2DOI
100 1 $aTÁVORA, F. T. P. K.
245 $aShotgun proteomics coupled to transient-inducible gene silencing reveal rice susceptibility genes as new sources for blast disease resistance.$h[electronic resource]
260 $c2021
500 $aNa publicação: Angela Mehta.
520 $aA comparative proteomic analysis between two near-isogenic rice lines, displaying a resistant and susceptible phenotype upon infection with Magnaporthe oryzae was performed. We identified and validated factors associ-ated with rice disease susceptibility, representing a flourishing source toward a more resolute rice-blast resis-tance. Proteome profiles were remarkably different during early infection (12 h post-inoculation), revealing several proteins with increased abundance in the compatible interaction. Potential players of rice susceptibility were selected and gene expression was evaluated by RT-qPCR. Gene Ontology analysis disclosed susceptibility gene-encoded proteins claimed to be involved in fungus sustenance and suppression of plant immunity, such as sucrose synthase 4-like, serpin-ZXA-like, nudix hydrolase15, and DjA2 chaperone protein. Two other candidate genes, picked from a previous transcriptome study, were added into our downstream analysis including pyr-abactin resistant-like 5 (OsPYL5), and rice ethylene-responsive factor 104 (OsERF104). Further, we validated their role in susceptibility by Transient-Induced Gene Silencing (TIGS) using short antisense oligodeoxyr-ibonucleotides that resulted in a remarkable reduction of foliar disease symptoms in the compatible interaction. Therefore, we successfully employed shotgun proteomics and antisense-based gene silencing to prospect and functionally validate rice potential susceptibility factors, which could be further explored to build rice-blast resistance. Significance: R gene-mediated disease resistance is race-specific and often not durable in the field. More recently, advancements in new breeding techniques (NBTs) have made plant disease susceptibility genes (S-genes) a new target to build a broad spectrum and more durable resistance, hence an alternative source to R-genes in breeding programs. We successfully coupled shotgun proteomics and gene silencing tools to prospect and validate new rice-bast susceptibility genes that can be further exploited toward a more resolute blast disease resistance.
650 $ablast disease
650 $adisease resistance
650 $afoliar diseases
650 $aMagnaporthe oryzae
650 $aplant diseases and disorders
650 $aproteomics
650 $arice
650 $aArroz
650 $aBrusone
650 $aDoença de Planta
650 $aOryza Sativa
653 $aGene ontology
653 $aPlant immunity
653 $aPTO-based TIGS
653 $aRT-qPCR
653 $aS-gene
653 $aTransient-Induced gene silencing
700 1 $aBEVITORI, R.
700 1 $aMELLO, R. N. de
700 1 $aCINTRA, M. M. D. F.
700 1 $aOLIVEIRA NETO, O. B.
700 1 $aFONTES, W.
700 1 $aCASTRO, M. S.
700 1 $aSOUSA, M. V.
700 1 $aFRANCO, O. L.
700 1 $aREIS, A. M. dos
773 $tJournal of Proteomics$gv. 241, 104223, June 2021.
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Embrapa Arroz e Feijão (CNPAF) |
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| 8. |  | NOVAES, J. J. da S.; FERREIRA, F. G.; LEITE, L. C.; CAMPOS, V.; RODRIGUES, V. O.; SANTOS, J. de J. dos; MAZZA, P. H. S.; OLIVEIRA, E. J. de. Fracionamento de carboidratos da parte aérea de diferentes variedades de mandioca. CONGRESO DE LA ASOCIACIÓN LATINOAMERICANA DE PRODUCCIÓN ANIMAL, 28; CONGRESO DE PRODUCCIÓN ANIMAL DEL NORDESTE 11 [2016], [Recife], La seguridade alimentaria em América Latina. [Recife], [2016].| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 9. | | CARVALHO, J. O. P. de; SANTOS, J. de J.; SALDANHA, E. B.; MELO, A. do S. G. de; NASCIMENTO, S. M. do; HIRAI, E. H.; CASTRO, T. da C.; COELHO, A. A.; BATISTA, R. W. C. Identificação botânica de espécies arbóreas pouco conhecidas como contribuição ao planejamento do manejo de florestas naturais no Estado do Pará. In: CONFERÊNCIA DO SUBPROGRAMA DE CIÊNCIA E TECNOLOGIA - SPC&T FASE II/PPG7, 2008, Belém, PA. Anais... Brasília, DF: CNPq, 2009. p. 334-338. Disponível também em CD-ROM (CD 00173).| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 10. | | GARCIA, A. O.; FERREIRA, F. G.; LEITE, L. C.; ASSIS, M. T. S. de; FARIAS, G. de C.; SANTOS, J. de J. dos; RODRIGUES, V. O.; OLIVEIRA, E. J. de. Composição químico - bromatológica da rama de diferentes variedades de mandioca aos 18 meses de idade. CONGRESO DE LA ASOCIACIÓN LATINOAMERICANA DE PRODUCCIÓN ANIMAL, 28; CONGRESO DE PRODUCCIÓN ANIMAL DEL NORDESTE 11 [2016], [Recife], La seguridade alimentaria em América Latina. [Recife], [2016].| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 10 | |
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