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 | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
11/12/2020 |
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Data da última atualização: |
11/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARQUES, T. C.; SANTOS, E. C. da S.; DIESEL, T. O.; MARTINS, C. F.; CUMPA, H. C. B.; LEME, L. de O.; DODE, M. A. N.; ALVES, B. G.; COSTA, F. P. H.; OLIVEIRA, E. B.; GAMBARINI, M. L. |
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Afiliação: |
CARLOS FREDERICO MARTINS, CPAC; MARGOT ALVES NUNES DODE, Cenargen. |
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Título: |
Blastocoel fluid removal and melatonin supplementation in the culture medium improve the viability of vitrified bovine embryos. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Theriogenology, Vv. 160, p. 134-141, 2021. |
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Páginas: |
p. 134-141 |
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ISSN: |
0093-691X |
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Idioma: |
Português |
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Conteúdo: |
In this study, we investigated the effects of melatonin supplementation in the culture medium and blastocoel fluid removal (BFR) before vitrification on the quality and viability of in vitro-derived bovine embryos. After fertilization, presumptive zygotes were assigned to one of the following treatments: control, in vitro standard culture (IVC) medium; IVC þ M109 , IVC medium supplemented 109 M melatonin; or IVC þ M109 BFR, IVC medium supplemented with 109 M melatonin plus BFR on day 7 (D7) of culture. D7 blastocysts were vitrified by the Cryotop method and, after 5 mo of storage, were warmed and incubated for an additional 72 h. The re-expansion rate was evaluated after 2 and 24 h, and the hatching rate was evaluated after 24, 48, and 72 h. At 72 h, the total number of cells (TNC); number of apoptotic cells (NAC); and expression of genes related to oxidative stress (HSPA5), cell metabolism (SLC2A3), cell repair (MSH6), placentation (KRT8 and PLAC8), and implantation (FOSL1) were assessed in the blastocysts. Less than 30% of the control blastocysts re-expanded until 2 h, whereas more than 85% of the IVC þ M109 and IVC þ M109 BFR blastocysts re-expanded (P < 0.05). The hatching rate of IVC þ M109 BFR blastocysts increased at all time points (P < 0.05), reaching 66.8% at 72 h of incubation. The TNC was similar among treatments (P > 0.05), regardless of vitrification/warming and re-cultivation. The NAC:TNC was smaller for melatonin-treated blastocysts (P < 0.05). BFR increased HSPA5 (P ¼ 0.0118) expression and did not affect SLC2A3, MSH6, KRT8, and FOSL1 expression (P > 0.05). In conclusion, melatonin (109 M) supplementation in the culture medium and BFR on D7 of culture increased the hatching rate 24, 48, and 72 h after warming of the vitrified embryos, indicating an improvement in cryotolerance. MenosIn this study, we investigated the effects of melatonin supplementation in the culture medium and blastocoel fluid removal (BFR) before vitrification on the quality and viability of in vitro-derived bovine embryos. After fertilization, presumptive zygotes were assigned to one of the following treatments: control, in vitro standard culture (IVC) medium; IVC þ M109 , IVC medium supplemented 109 M melatonin; or IVC þ M109 BFR, IVC medium supplemented with 109 M melatonin plus BFR on day 7 (D7) of culture. D7 blastocysts were vitrified by the Cryotop method and, after 5 mo of storage, were warmed and incubated for an additional 72 h. The re-expansion rate was evaluated after 2 and 24 h, and the hatching rate was evaluated after 24, 48, and 72 h. At 72 h, the total number of cells (TNC); number of apoptotic cells (NAC); and expression of genes related to oxidative stress (HSPA5), cell metabolism (SLC2A3), cell repair (MSH6), placentation (KRT8 and PLAC8), and implantation (FOSL1) were assessed in the blastocysts. Less than 30% of the control blastocysts re-expanded until 2 h, whereas more than 85% of the IVC þ M109 and IVC þ M109 BFR blastocysts re-expanded (P < 0.05). The hatching rate of IVC þ M109 BFR blastocysts increased at all time points (P < 0.05), reaching 66.8% at 72 h of incubation. The TNC was similar among treatments (P > 0.05), regardless of vitrification/warming and re-cultivation. The NAC:TNC was smaller for melatonin-treated blastocysts (P < 0.05). BFR increased ... Mostrar Tudo |
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Palavras-Chave: |
Apoptose celular; Criotolerância; Fertilização; Melatonina; Vitrificação. |
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Thesagro: |
Cultura de Embrião; Embrião. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02843naa a2200349 a 4500
001 2127986
005 2020-12-11
008 2021 bl uuuu u00u1 u #d
022 $a0093-691X
100 1 $aMARQUES, T. C.
245 $aBlastocoel fluid removal and melatonin supplementation in the culture medium improve the viability of vitrified bovine embryos.$h[electronic resource]
260 $c2021
300 $ap. 134-141
520 $aIn this study, we investigated the effects of melatonin supplementation in the culture medium and blastocoel fluid removal (BFR) before vitrification on the quality and viability of in vitro-derived bovine embryos. After fertilization, presumptive zygotes were assigned to one of the following treatments: control, in vitro standard culture (IVC) medium; IVC þ M109 , IVC medium supplemented 109 M melatonin; or IVC þ M109 BFR, IVC medium supplemented with 109 M melatonin plus BFR on day 7 (D7) of culture. D7 blastocysts were vitrified by the Cryotop method and, after 5 mo of storage, were warmed and incubated for an additional 72 h. The re-expansion rate was evaluated after 2 and 24 h, and the hatching rate was evaluated after 24, 48, and 72 h. At 72 h, the total number of cells (TNC); number of apoptotic cells (NAC); and expression of genes related to oxidative stress (HSPA5), cell metabolism (SLC2A3), cell repair (MSH6), placentation (KRT8 and PLAC8), and implantation (FOSL1) were assessed in the blastocysts. Less than 30% of the control blastocysts re-expanded until 2 h, whereas more than 85% of the IVC þ M109 and IVC þ M109 BFR blastocysts re-expanded (P < 0.05). The hatching rate of IVC þ M109 BFR blastocysts increased at all time points (P < 0.05), reaching 66.8% at 72 h of incubation. The TNC was similar among treatments (P > 0.05), regardless of vitrification/warming and re-cultivation. The NAC:TNC was smaller for melatonin-treated blastocysts (P < 0.05). BFR increased HSPA5 (P ¼ 0.0118) expression and did not affect SLC2A3, MSH6, KRT8, and FOSL1 expression (P > 0.05). In conclusion, melatonin (109 M) supplementation in the culture medium and BFR on D7 of culture increased the hatching rate 24, 48, and 72 h after warming of the vitrified embryos, indicating an improvement in cryotolerance.
650 $aCultura de Embrião
650 $aEmbrião
653 $aApoptose celular
653 $aCriotolerância
653 $aFertilização
653 $aMelatonina
653 $aVitrificação
700 1 $aSANTOS, E. C. da S.
700 1 $aDIESEL, T. O.
700 1 $aMARTINS, C. F.
700 1 $aCUMPA, H. C. B.
700 1 $aLEME, L. de O.
700 1 $aDODE, M. A. N.
700 1 $aALVES, B. G.
700 1 $aCOSTA, F. P. H.
700 1 $aOLIVEIRA, E. B.
700 1 $aGAMBARINI, M. L.
773 $tTheriogenology, Vv. 160, p. 134-141, 2021.
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| 1. |  | PIRES, A. M. M.; MATTIAZZO, M. E.; BERTON, R. Àcidos orgânicos como extratores de metais pesados fitodisponíveis em solos tratados com lodo de esgoto. REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 26.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 10.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 8.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 5., 2004, Lages. Avaliação das conquistas: bases para estratégias futuras [anais]. Lages: UDESC: SBCS, 2004. CD-ROM. Fs 09.| Biblioteca(s): Embrapa Hortaliças. |
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| 8. | | GONZÁLEZ PÉREZ, M.; COLNAGO, L. A.; MARTIN-NETO, L.; BERTON, R.; BETTIOL, W. CAMARGO, O. A. RMN de 13C de solos intactos e frações granulométricas para estudos de matéria orgânica. In: JORNADA BRASILEIRA DE RESSONÂNCIA MAGNÉTICA,7.; WORKSHOP "APLICAÇÕES DA RMN", 2002, Maringá. Resumos... [Rio de Janeiro]: AUREMN: UFRJ-Instituto de Química, 2002. p. 115-116.| Biblioteca(s): Embrapa Instrumentação. |
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| 12. | | SILVA, F. C. da; SANTOS, A. D. dos; BERTON, R. S. Gestão pública de resíduo sólido domiciliar orgânico no município: compostagem, agricultura e informática. In: MAGNONI JÚNIOR, L.; STEVENS, D.; SILVA, W. T. L. da; VALE, J. M. F. do; PURINI, S. R. de M.; MAGNONI, M. da G. M.; SEBASTIÃO, E.; BRANCO JÚNIOR, G.; ADORNO FILHO, E. F.; FIGUEIREDO, W. dos S.; SEBASTIÃO, I. (Org.). JC na Escola Ciência, Tecnologia e Sociedade: mobilizar o conhecimento para alimentar o Brasil. 2. ed. São Paulo: Centro Paula Souza, 2017. p. 111-139.| Tipo: Artigo em Anais de Congresso |
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| 14. | | SILVA, F. C. da; BERTON, R. S.; CHITOLINA, J. C.; BALLESTERO, S. D.; BARIONI, L. Establishment of recommendations and parameters for use of urban waste compost in agriculture in the State of Sao Paulo - Brazil. In: INTERNATIONAL ORBIT CONFERENCE, 7., 2010, Heraklion Crete. Organic resources in the carbon economy: proceedings. Thessaloniki, Greece: Grafima, 2010. p. 1246. Poster. Orbit 2010.| Tipo: Resumo em Anais de Congresso |
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| 18. | | GONZÁLEZ PÉREZ, M.; MILORI, D. M. B. P.; MARTIN-NETO, L.; BERTON, R.; BETTIOL, W.; CAMARGO, O. A. Avaliação das frações granulométricas de um latossolo vermelho amarelo tratado com lodo de esgoto através da fluorescência induzida por laser. In: REUNIÃO BRASILEIRA DE MANEJO E CONSERVAÇÃO DO SOLO E DA ÁGUA, 14., 2002, Cuiabá. Anais... [Cuiabá: SBCS: UFMT], 2002. 4 f. 1 CD-ROM.| Biblioteca(s): Embrapa Instrumentação. |
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| 19. | | GONZÁLEZ-PÉREZ, M.; MILORI, D. M. B. P.; MARTIN-NETO, L.; BERTON, R.; BETTIOL, W.; CAMARGO, O. A. Fluorescência induzida por laser para avaliar matéria orgânica de solos sob aplicação de lodo de esgoto. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE QUÍMICA: REFLEXÃO CRÍTICA E PERSPECTIVAS, 25., 2002, Caxambu. Livro de resumos... Poços de Caldas: [Sociedade Brasileira de Química]., 2002. Não-paginado, ref. AB-011.| Biblioteca(s): Embrapa Instrumentação. |
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| 20. | | SILVA, F. C. da; BOARETTO, A. E.; BERTON, R. S.; ZOTELLI, H. B.; PEXE, C. A.; MENDONCA, E. Cana-de-açúcar cultivada em solo adubado com lodo de esgoto: nutrientes, metais pesados e produtividade. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 33, n. 1, p. 1-8, jan. 1998.| Biblioteca(s): Embrapa Solos; Embrapa Unidades Centrais. |
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| Registros recuperados : 47 | |
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