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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sul. Para informações adicionais entre em contato com cppsul.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sul. |
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Data corrente: |
27/11/2020 |
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Data da última atualização: |
27/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
COMIN, H. B.; DOMINGUES, R.; GASPAR, E. B.; SANTOS, J. R. G. de los; CARDOSO, F. F. |
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Afiliação: |
Helena Brocardo Comin, UFPEL; ROBERT DOMINGUES, CPPSUL; EMANUELLE BALDO GASPAR, CPPSUL; João Rodrigo Gil De Los Santos, UFPEL; FERNANDO FLORES CARDOSO, CPPSUL. |
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Título: |
Genetic differences among Moraxella bovis and Moraxella bovoculi isolates from infectious bovine keratoconjunctivitis (IBK) outbreaks in southern Brazil. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, v. 43, n. 2, e20180380, p. 1-7, 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to evaluate the genetic diversity ofMoraxella bovisandMoraxella bovoculibacteriaisolated from infectious bovine keratoconjunctivitis (IBK) outbreaks in the state of Rio Grande do Sul, Brazil. The ge-netic diversity amongMoraxellaspp. was evaluated by RAPD-PCR, JWP1-JWOPA07-PCR, ERIC-PCR and by se-quencing the 16S-23S intergenic regions. Based on the dendrogram, two genetically differentiated clades wereobserved; 14 isolates were classified asM. bovisand 17 asM. bovoculi. Genetic distances between theM. bovissamples ranged from 0.0379 to 0.4285, while forM. bovoculithe dissimilarities ranged from zero to 0.7297. Alterna-tively, based on sequencing analyses of the 16S-23S intergenic region,M. bovisandM. bovoculiisolates weregrouped into the same two different clades, but it was not possible to differentiate between isolates within clades.PCR techniques were demonstrated to be a satisfactory tool to unravel the genetic variability amongMoraxellaspp.,while sequencing of the 16S-23S intergenic region was only able to differentiate two species of theMoraxellagenus.Despite sampling geographically close regions, we demonstrate considerable genetic diversity inM. bovisandM.bovoculistrains and genetically distinctM. bovisstrains co-infecting the same animal. |
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Thesagro: |
Bovino; Doença Animal; Genética Animal; Olho. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02007naa a2200217 a 4500
001 2127157
005 2020-11-27
008 2020 bl uuuu u00u1 u #d
100 1 $aCOMIN, H. B.
245 $aGenetic differences among Moraxella bovis and Moraxella bovoculi isolates from infectious bovine keratoconjunctivitis (IBK) outbreaks in southern Brazil.$h[electronic resource]
260 $c2020
520 $aThe objective of this study was to evaluate the genetic diversity ofMoraxella bovisandMoraxella bovoculibacteriaisolated from infectious bovine keratoconjunctivitis (IBK) outbreaks in the state of Rio Grande do Sul, Brazil. The ge-netic diversity amongMoraxellaspp. was evaluated by RAPD-PCR, JWP1-JWOPA07-PCR, ERIC-PCR and by se-quencing the 16S-23S intergenic regions. Based on the dendrogram, two genetically differentiated clades wereobserved; 14 isolates were classified asM. bovisand 17 asM. bovoculi. Genetic distances between theM. bovissamples ranged from 0.0379 to 0.4285, while forM. bovoculithe dissimilarities ranged from zero to 0.7297. Alterna-tively, based on sequencing analyses of the 16S-23S intergenic region,M. bovisandM. bovoculiisolates weregrouped into the same two different clades, but it was not possible to differentiate between isolates within clades.PCR techniques were demonstrated to be a satisfactory tool to unravel the genetic variability amongMoraxellaspp.,while sequencing of the 16S-23S intergenic region was only able to differentiate two species of theMoraxellagenus.Despite sampling geographically close regions, we demonstrate considerable genetic diversity inM. bovisandM.bovoculistrains and genetically distinctM. bovisstrains co-infecting the same animal.
650 $aBovino
650 $aDoença Animal
650 $aGenética Animal
650 $aOlho
700 1 $aDOMINGUES, R.
700 1 $aGASPAR, E. B.
700 1 $aSANTOS, J. R. G. de los
700 1 $aCARDOSO, F. F.
773 $tGenetics and Molecular Biology$gv. 43, n. 2, e20180380, p. 1-7, 2020.
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