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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Soja; Embrapa Suínos e Aves. |
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Data corrente: |
11/11/2020 |
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Data da última atualização: |
18/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, E.; GRAÇA, J. P. da; PORTO, C.; PRADO, R. M.; HOFFMANN-CAMPO, C. B.; MEYER, M. C.; NUNES, E. de O.; PILAU, E. J. |
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Afiliação: |
Evandro Silva, Universidade Estadual de Maringá, UEM, Maringá, PR.; José Perez da Graça, Universidade Estadual de Maringá, UEM, Maringá, PR.; Carla Porto, Universidade Estadual de Maringá, UEM, Maringá, PR.; Rodolpho Martin do Prado, Universidade Estadual de Maringá, UEM, Maringá, PR.; CLARA BEATRIZ HOFFMANN CAMPO, CNPSO; MAURICIO CONRADO MEYER, CNPSO; ESTELA DE OLIVEIRA NUNES, CNPSA; Eduardo Jorge Pilau, Universidade Estadual de Maringá, UEM, Maringá, PR. |
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Título: |
Unraveling Asian Soybean Rust metabolomics using mass spectrometry and Molecular Networking approach. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Scientific Reports, v. 10, n. 138, 2020. |
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Páginas: |
11 p. |
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DOI: |
10.1038/s41598-019-56782-4 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Asian Soybean Rust (ASR), caused by the biotrophic fungus Phakopsora pachyrhizi, is a devastating disease with an estimated crop yield loss of up to 90%. Yet, there is a nerf of information on the metabolic response of soybean plants to the pathogen Untargeted metabolomics and Global Natural Products Social Molecular Networking platform approach was used to explore soybean metabolome modulation to P. pachyrhizi infection. Soybean plants susceptible to ASR was inoculated with P. pachyrhizi spore suspension and non-inoculated plants were used as controls. Leaves from both groups were collected 14 days post-inoculation and extracted using different extractor solvent mixtures. The extracts were analyzed on an ultra-high performance liquid chromatography system coupled to high-definition electrospray ionization-mass spectrometry. There was a significant production of defense secondary metabolites (phenylpropanoids, terpenoids and flavonoids) when P. pachyrhizi infected soybean plants, such as putatively identified liquiritigenin, coumestrol, formononetin, pisatin, medicarpin, biochanin A, glyoceollidin I, glyoceollidin II, glyoceollin I, glyoceolidin II, glyoceolidin III, glyoceolidin IV, glyoceolidin VI. Primary metabolites (amino acids, peptides and lipids) also were putatively identified. This is the first report using untargeted metabolomics and GNPS-Molecular Networking approach to explore ASR in soybean plants. Our data provide insights into the potential role of some metabolites in the plant resistance to ASR, which could result in the development of resistant genotypes of soybean to P. pachyrhizi, and effective and specific products against the pathogen. MenosAbstract: Asian Soybean Rust (ASR), caused by the biotrophic fungus Phakopsora pachyrhizi, is a devastating disease with an estimated crop yield loss of up to 90%. Yet, there is a nerf of information on the metabolic response of soybean plants to the pathogen Untargeted metabolomics and Global Natural Products Social Molecular Networking platform approach was used to explore soybean metabolome modulation to P. pachyrhizi infection. Soybean plants susceptible to ASR was inoculated with P. pachyrhizi spore suspension and non-inoculated plants were used as controls. Leaves from both groups were collected 14 days post-inoculation and extracted using different extractor solvent mixtures. The extracts were analyzed on an ultra-high performance liquid chromatography system coupled to high-definition electrospray ionization-mass spectrometry. There was a significant production of defense secondary metabolites (phenylpropanoids, terpenoids and flavonoids) when P. pachyrhizi infected soybean plants, such as putatively identified liquiritigenin, coumestrol, formononetin, pisatin, medicarpin, biochanin A, glyoceollidin I, glyoceollidin II, glyoceollin I, glyoceolidin II, glyoceolidin III, glyoceolidin IV, glyoceolidin VI. Primary metabolites (amino acids, peptides and lipids) also were putatively identified. This is the first report using untargeted metabolomics and GNPS-Molecular Networking approach to explore ASR in soybean plants. Our data provide insights into the potential role of ... Mostrar Tudo |
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Palavras-Chave: |
Espectrometria de massa; Ferrugem asiática. |
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Thesagro: |
Ferrugem; Soja. |
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Thesaurus Nal: |
Mass spectrometry; Soybean rust. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02549naa a2200301 a 4500
001 2126485
005 2020-12-18
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1038/s41598-019-56782-4$2DOI
100 1 $aSILVA, E.
245 $aUnraveling Asian Soybean Rust metabolomics using mass spectrometry and Molecular Networking approach.$h[electronic resource]
260 $c2020
300 $a11 p.
520 $aAbstract: Asian Soybean Rust (ASR), caused by the biotrophic fungus Phakopsora pachyrhizi, is a devastating disease with an estimated crop yield loss of up to 90%. Yet, there is a nerf of information on the metabolic response of soybean plants to the pathogen Untargeted metabolomics and Global Natural Products Social Molecular Networking platform approach was used to explore soybean metabolome modulation to P. pachyrhizi infection. Soybean plants susceptible to ASR was inoculated with P. pachyrhizi spore suspension and non-inoculated plants were used as controls. Leaves from both groups were collected 14 days post-inoculation and extracted using different extractor solvent mixtures. The extracts were analyzed on an ultra-high performance liquid chromatography system coupled to high-definition electrospray ionization-mass spectrometry. There was a significant production of defense secondary metabolites (phenylpropanoids, terpenoids and flavonoids) when P. pachyrhizi infected soybean plants, such as putatively identified liquiritigenin, coumestrol, formononetin, pisatin, medicarpin, biochanin A, glyoceollidin I, glyoceollidin II, glyoceollin I, glyoceolidin II, glyoceolidin III, glyoceolidin IV, glyoceolidin VI. Primary metabolites (amino acids, peptides and lipids) also were putatively identified. This is the first report using untargeted metabolomics and GNPS-Molecular Networking approach to explore ASR in soybean plants. Our data provide insights into the potential role of some metabolites in the plant resistance to ASR, which could result in the development of resistant genotypes of soybean to P. pachyrhizi, and effective and specific products against the pathogen.
650 $aMass spectrometry
650 $aSoybean rust
650 $aFerrugem
650 $aSoja
653 $aEspectrometria de massa
653 $aFerrugem asiática
700 1 $aGRAÇA, J. P. da
700 1 $aPORTO, C.
700 1 $aPRADO, R. M.
700 1 $aHOFFMANN-CAMPO, C. B.
700 1 $aMEYER, M. C.
700 1 $aNUNES, E. de O.
700 1 $aPILAU, E. J.
773 $tScientific Reports$gv. 10, n. 138, 2020.
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Esconder MarcMostrar Marc Completo |
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Registro original: |
Embrapa Soja (CNPSO) |
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Biblioteca |
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| Registros recuperados : 18 | |
| 3. |  | CAMPOS, C. G.; COSTA, P. P. K. G.; ABDELNUR, P. V.; MIRANDA, C. H. B. Desenvolvimento de método para identificação e quantificação de fito-hormônios produzidos por macro e microalgas utilizando UHPLC-MS/MS. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 6., 2020, Brasília, DF. Anais... Brasília, DF: Embrapa, 2020. p. 188-194 il.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 4. | | MALINOVSKI, L. I.; SILVA, T. C. da; CAMPOS, C. G. C.; FELDBERG, N. P.; STEFANINI, M.; SILVA, A. L. da. Comportamento vitícola da variedade ancellotta (vitis vinifera l.) em região de elevada altitude – Água Doce/SC. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 22., 2012, Bento Gonçalves. Anais... Bento Gonçalves: SBF, 2012. 4 p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 7. | | CAMPOS, C. G.; NASCIMENTO, I. F.; VERAS, H. C. T.; ALMEIDA, J. R. M. de; PARACHIN, N.; ABDELNUR, P. V. Desenvolvimento de protocolo para análise estatística de metabolômica de leveduras por ANOVA. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 5., 2018, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2018. p. 33.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 8. | | CAMPOS, C. G.; RIBEIRO, J. A. de A.; COSTA, P. P. K. G.; RODRIGUES, C. M.; ABDELNUR, P. V. Otimização de métodos cromatográficos para separação de ácidos orgânicos, monossacarídeos, álcoois e açúcares fosfatados. In: CONGRESSO BRASILEIRO DE QUÍMICA, 55., 2015, Goiânia. Recursos renováveis: inovação e tecnologia. Rio de Janeiro: ABQ, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 10. | | VERAS, H. C. T.; CAMPOS, C. G.; RIBEIRO, J. A. de A.; ABDELNUR, P. V.; PARACHIN, N. S.; ALMEIDA, J. R. M. de. Limiting steps for xylose metabolism in yeast: comparative fermentation performance and metabolomics. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 2017, 39., San Francisco, US. [Proceedings ...]. Fairfax: Society for Industrial Microbiology and Biotechnology, 2017. Não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
|  |
| 13. | | CAMPOS, C. G.; VERAS, H. C. T.; RIBEIRO, J. A. de A.; COSTA, P. P. K. G.; RODRIGUES, C. M.; ALMEIDA, J. R. M. de; ABDELNUR, P. V. Preparo de amostra em metabolômica de leveduras para a determinação dos metabólitos produzidos na fermentação de xilose. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 2., 2015, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2015. p. 81-83| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 14. | | CAMPOS, C. G.; RIBEIRO, J. A. de A.; COSTA, P. P. K. G.; PEREIRA, K. A.; VERAS, H. C.; ALMEIDA, J. R. M. de; RODRIGUES, C. M.; ABDELNUR, P. V. Análise quantitativa dos metabólitos produzidos por levedura na via de fermentação da xilose utilizando UHPLC-MS/MS. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE QUÍMICA, 39., 2016, Goiânia, GO. Criar e empreender: anais ... São Paulo: SBQ, 2016. Não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 15. | | CAMPOS, C. G.; VERAS, H. C. T.; RIBEIRO, J. A. de A.; COSTA, P. P. K. G.; ARAÚJO, K. P.; RODRIGUES, C. M.; ALMEIDA, J. R. M. de; ABDELNUR, P. V. New protocol based on UHPLC-MS/MS for quantitation of metabolites in xylose-fermenting yeasts. Journal of the American Society for Mass Spectrometry, v. 28, n. 12, p. 2646-2657, 2017. p. 2646-2657| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agroenergia. |
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| 16. | | CAMPOS, C. G.; RIBEIRO, J. A. de A.; VERAS, H. C. T.; COSTA, P. P. K. G.; ARAÚJO, K. P.; ALMEIDA, J. R. M. de; RODRIGUES, C. M.; ABDELNUR, P. V. Identificação de alvos moleculares na via metabólica de fermentação de xilose utilizando estratégias de metabolômica. In: WORKSHOP DA PÓS-GRADUAÇÃO EM QUÍMICA, 1., 2015, Goiânia, GO. [Resumos ...] Goiânia: UFG, 2015. WSPGQ-027| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 17. | | BAHIA, F. M.; ALMEIDA, G. C. de; ANDRADE, L. P. de; CAMPOS, C. G.; QUEIROZ, L. R.; SILVA, L. R. V. da; ABDELNUR, P. V.; CORRÊA, J. R.; BETTIGA, M.; PARACHIN, N. S. Rhamnolipids production from sucrose by engineered Saccharomyces cerevisiae. Scientific Reports, v. 8, artigo 2905, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroenergia. |
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| 18. | | PEREIRA, C. B.; SILVA, R. M. C. da; POLETTO, C. M.; CAMPOS, C. G.; RIBEIRO, J. A. de A.; RODRIGUES, C. M.; ABDELNUR, P. V.; ALMEIDA, J. R. M. de; DAMASO, M. C. T. Screening of microorganisms for production of aggregated-value chemicals from glycerin bioconversion. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 37., 2015, San Diego, California. [Resumos ...] Fairfax: Society for Industrial Microbiology and Biotechnology, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| Registros recuperados : 18 | |
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