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Registro Completo |
Biblioteca(s): |
Embrapa Suínos e Aves. |
Data corrente: |
04/11/2020 |
Data da última atualização: |
04/11/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ATKINSON, B. M.; BEARSON, B. L.; LOVING, C. L.; ZIMMERMANN, J. J.; KICH, J. D.; BEARSON S. M. D. |
Afiliação: |
BRIONY M. ATKINSON, USDA/ARS; USDA/ARS; USDA/ARS; USDA/ARS; JALUSA DEON KICH, CNPSA; USDA/ARS. |
Título: |
Detection of salmonella-specif antibody in swine oral fluids. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Porcine Health Management, v.5, n. 29, 2019. |
DOI: |
https://doi.org/10.1186/s40813-019-0136-7 |
Idioma: |
Inglês |
Palavras-Chave: |
Detecção de anticorpos; Fluidos orais. |
Thesagro: |
Salmonella; Suíno. |
Thesaurus Nal: |
Antibody detection; Swine. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00734naa a2200253 a 4500 001 2126218 005 2020-11-04 008 2019 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1186/s40813-019-0136-7$2DOI 100 1 $aATKINSON, B. M. 245 $aDetection of salmonella-specif antibody in swine oral fluids.$h[electronic resource] 260 $c2019 650 $aAntibody detection 650 $aSwine 650 $aSalmonella 650 $aSuíno 653 $aDetecção de anticorpos 653 $aFluidos orais 700 1 $aBEARSON, B. L. 700 1 $aLOVING, C. L. 700 1 $aZIMMERMANN, J. J. 700 1 $aKICH, J. D. 700 1 $aBEARSON S. M. D. 773 $tPorcine Health Management$gv.5, n. 29, 2019.
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Embrapa Suínos e Aves (CNPSA) |
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Registro Completo
Biblioteca(s): |
Embrapa Pesca e Aquicultura. |
Data corrente: |
28/04/2021 |
Data da última atualização: |
28/04/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
CORNACINI, M. R.; MANOEL, R. O.; ALCANTARA, M. A. M.; MORAES, M. L. T.; SILVA, E. A. A.; PEREIRA NETO, L. G.; SEBBENN, A. M.; ROSSINI, B. C.; MARINO, C. L. |
Afiliação: |
MAIARA R. CORNACINI, UNESP, Botucatu-SP; RICARDO O. MANOEL, UNESP, Botucatu-SP; MARCELO A. M. ALCANTARA, UNESP, Botucatu-SP; MARIO L. T. MORAES, UNESP, Ilha Solteira-SP; EDVALDO A. A. SILVA, UNESP, Botucatu-SP; LEONEL GONCALVES PEREIRA NETO, CNPASA; ALEXANDRE M. SEBBENN, INSTITUTO FLORESTAL DE SÃO PAULO, Piracicaba-SP; BRUNO C. ROSSINI, UNESP, Botucatu-SP; CELSO L. MARINO, UNESP, Botucatu-SP. |
Título: |
Detection and application of novel SSR markers from transcriptome data for Astronium fraxinifolium Schott, a threatened Brazilian tree species. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Molecular Biology Reports, 2021. |
ISSN: |
0301-4851 |
DOI: |
https://doi.org/10.1007/s11033-021-06338-5 |
Idioma: |
Inglês |
Conteúdo: |
Astronium fraxinifolium is an endangered tree species from Brazil. Due to its significance in environmental reforestation, as well as the continued exploitation of its wood, it is necessary to develop management programs that support the conservation of the species. Simple sequence repeats (SSR) or microsatellite markers are widely used in population genetic studies across a range of diverse organisms. In this study, we present the first SSR markers developed for A. fraxinifolium as well as their frequency and distribution based on transcriptome data. From transcriptome data, we identified more than 100 thousand sequences presenting microsatellites, with a predominant distribution of trinucleotide repeats. From the initial screening, we selected 20 microsatellite loci which were validated and evaluated for genetic indices in two natural populations. All loci were polymorphic, ranging from four to 11 alleles per locus. The observed and expected heterozygosities ranged from 0 to 1.0 and from 0.533 to 1.0, respectively, while the genetic differentiation (GST = 0.363) was greater within than between populations. The developed SSR loci from RNA-Seq data provides a foundation for future studies on genetic diversity and population structure, mating system, and gene flow for A. fraxinifolium populations and related species, aiming at conservation and management. |
Palavras-Chave: |
Conservation genetics; Microsatellite markers. |
Thesagro: |
Anacardiaceae; Astronium Fraxinifolium. |
Thesaurus NAL: |
Conservation plants; Genetic markers; Microsatellite repeats; Population genetics. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/222904/1/mbr-2021.pdf
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Marc: |
LEADER 02452naa a2200337 a 4500 001 2131540 005 2021-04-28 008 2021 bl uuuu u00u1 u #d 022 $a0301-4851 024 7 $ahttps://doi.org/10.1007/s11033-021-06338-5$2DOI 100 1 $aCORNACINI, M. R. 245 $aDetection and application of novel SSR markers from transcriptome data for Astronium fraxinifolium Schott, a threatened Brazilian tree species.$h[electronic resource] 260 $c2021 520 $aAstronium fraxinifolium is an endangered tree species from Brazil. Due to its significance in environmental reforestation, as well as the continued exploitation of its wood, it is necessary to develop management programs that support the conservation of the species. Simple sequence repeats (SSR) or microsatellite markers are widely used in population genetic studies across a range of diverse organisms. In this study, we present the first SSR markers developed for A. fraxinifolium as well as their frequency and distribution based on transcriptome data. From transcriptome data, we identified more than 100 thousand sequences presenting microsatellites, with a predominant distribution of trinucleotide repeats. From the initial screening, we selected 20 microsatellite loci which were validated and evaluated for genetic indices in two natural populations. All loci were polymorphic, ranging from four to 11 alleles per locus. The observed and expected heterozygosities ranged from 0 to 1.0 and from 0.533 to 1.0, respectively, while the genetic differentiation (GST = 0.363) was greater within than between populations. The developed SSR loci from RNA-Seq data provides a foundation for future studies on genetic diversity and population structure, mating system, and gene flow for A. fraxinifolium populations and related species, aiming at conservation and management. 650 $aConservation plants 650 $aGenetic markers 650 $aMicrosatellite repeats 650 $aPopulation genetics 650 $aAnacardiaceae 650 $aAstronium Fraxinifolium 653 $aConservation genetics 653 $aMicrosatellite markers 700 1 $aMANOEL, R. O. 700 1 $aALCANTARA, M. A. M. 700 1 $aMORAES, M. L. T. 700 1 $aSILVA, E. A. A. 700 1 $aPEREIRA NETO, L. G. 700 1 $aSEBBENN, A. M. 700 1 $aROSSINI, B. C. 700 1 $aMARINO, C. L. 773 $tMolecular Biology Reports, 2021.
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