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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia; Embrapa Gado de Leite. |
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Data corrente: |
28/10/2020 |
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Data da última atualização: |
28/08/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CAMELO, A.; BARRETO, C. P.; VIDAL, M. S.; ROUWS, J. R. C.; LEDO, F. J. da S.; SCHWAB, S.; BALDANI, J. I. |
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Afiliação: |
Alessandra Camelo, USP; Camila Paes Barreto, USP; MARCIA SOARES VIDAL, CNPAB; JANAINA RIBEIRO COSTA ROUWS, CNPAB; FRANCISCO JOSE DA SILVA LEDO, CNPGL; STEFAN SCHWAB, CNPAB; JOSE IVO BALDANI, CNPAB. |
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Título: |
Field response of two seed propagated elephant grass genotypes to diazotrophic bacterial inoculation and in situ confocal microscopy colonization analyses. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Symbiosis, v. 83, n. 1, p. 41-53, 2021 |
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DOI: |
https://doi.org/10.1007/s13199-020-00730-8 |
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Idioma: |
Inglês |
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Conteúdo: |
Elephant grass (Pennisetum purpureum) is a perennial C4-plant with outstanding sustainable potential for bioenergy, grazing and silage, It is able to fix nitrogen in association with diazotrophic bacteria and is a high biomass producer, even in low fertility soils. This study aimed to investigate the response of two elephant grass genotypes (PCEA and PMN hybrid) to inoculation, via seed coat and leaf spray, with Azospirillum baldaniorum (Sp245) and Gluconacetobacter diazotrophicus (LP343), grown in the field. In addition, in situ colonization of field PCEA minisets with labeled mCherry strain LP343 and gfp strain Sp245 was analyzed. The results showed that the PCEA genotype produced a higher amount of biomass and total N than the PMN hybrid when inoculated with strain Sp245 on seeds and strain LP343 sprayed on leaves. The quantification of the 15N abundance (δ15N) showed no biological nitrogen fixation (BNF) contribution by either of the inoculated strains and mode of application in leaves of PCEA genotype. The microscopy confocal analysis showed internal tissue colonization of sprouted PCEA minisets by strain LP343 while detection of Sp245 was reduced during the assays. In contrast, leaf surface spot inoculation showed no internal tissue colonization by either strain although the LP343 strain remained longer on the leaf surface. The heavy miniset plant tissue colonization by LP343 (roots and leaves) could be related to its host specificity as compared to Sp245 and may therefore be responsible for the higher increase in the total N accumulated in the plant superior to N fertilization. This effect could, however, involve mechanisms other than the BNF process. MenosElephant grass (Pennisetum purpureum) is a perennial C4-plant with outstanding sustainable potential for bioenergy, grazing and silage, It is able to fix nitrogen in association with diazotrophic bacteria and is a high biomass producer, even in low fertility soils. This study aimed to investigate the response of two elephant grass genotypes (PCEA and PMN hybrid) to inoculation, via seed coat and leaf spray, with Azospirillum baldaniorum (Sp245) and Gluconacetobacter diazotrophicus (LP343), grown in the field. In addition, in situ colonization of field PCEA minisets with labeled mCherry strain LP343 and gfp strain Sp245 was analyzed. The results showed that the PCEA genotype produced a higher amount of biomass and total N than the PMN hybrid when inoculated with strain Sp245 on seeds and strain LP343 sprayed on leaves. The quantification of the 15N abundance (δ15N) showed no biological nitrogen fixation (BNF) contribution by either of the inoculated strains and mode of application in leaves of PCEA genotype. The microscopy confocal analysis showed internal tissue colonization of sprouted PCEA minisets by strain LP343 while detection of Sp245 was reduced during the assays. In contrast, leaf surface spot inoculation showed no internal tissue colonization by either strain although the LP343 strain remained longer on the leaf surface. The heavy miniset plant tissue colonization by LP343 (roots and leaves) could be related to its host specificity as compared to Sp245 and may ... Mostrar Tudo |
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Palavras-Chave: |
Azospirillum baldaniorum; Biological Resource Center Embrapa Agrobiologia; Biomass accumulation; FBN. |
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Thesagro: |
Biomassa; Capim Elefante; Inoculação; Pennisetum Purpureum. |
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Thesaurus Nal: |
Azospirillum; Gluconacetobacter. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
null
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| Registros recuperados : 2 | |
| 1. |  | LIMA, A. de A.; CALDAS, R. C.; BORGES, A. L.; RITZINGER, C. H. S. P.; TRINDADE, A. V.; PIRES, M. de M.; MIDLEJ, M. M. B. C.; MATA, H. T. da C. Cultivos intercalares e controle de plantas daninhas em plantios de maracujá amarelo - II. Cruz das Almas: Embrapa Mandioca e Fruticultura, 2003. 4 p. ( Embrapa Mandioca e Fruticultura. Comunicado Técnico, 89).| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 2. | | LIMA, A. de A.; CALDAS, R. C.; BORGES, A. L.; RITZINGER, C. H. S. P.; TRINDADE, A. V.; PIRES, M. de M.; MIDLEJ, M. M. B. C.; MATA, H. T. da C. Cultivos intercalares e controle de plantas daninhas em plantios de maracujá amarelo. Cruz das Almas: Embrapa Mandioca e Fruticultura Tropical, 2003. 1 CD-ROM. (Embrapa Mandioca e Fruticultura. Boletim de Pesquisa e Desenvolvimento, 29).| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 2 | |
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