| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
|
Data corrente: |
03/08/2020 |
|
Data da última atualização: |
10/01/2025 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
BRAIR, V. L.; MAIA, A. L. R. S.; CORREIA, L. F. L.; BARBOSA, N. O.; SANTOS, J. D. R.; BRANDÃO, F. Z.; FONSECA, J. F. da; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G. |
|
Afiliação: |
VIVIANE L. BRAIR, UNIVERSIDADE FEDERAL FLUMINENSE; ANA LUCIA R. S. MAIA, UNIVERSIDADE FEDERAL FLUMINENSE; LUCAS FRANCISCO L. CORREIA, UNIVERSIDADE FEDERAL FLUMINENSE; NATHALIA O. BARBOSA, UNIVERSIDADE FEDERAL FLUMINENSE; JULIANA D. R. SANTOS, UNIVERSIDADE FEDERAL FLUMINENSE; FELIPE Z. BRANDÃO, UNIVERSIDADE FEDERAL FLUMINENSE; JEFERSON FERREIRA DA FONSECA, CNPC; RIBRIO IVAN T. P. BATISTA, UNIVERSIDADE FEDERAL FLUMINENSE; JOANNA M. G. SOUZA-FABJAN, UNIVERSIDADE FEDERAL FLUMINENSE. |
|
Título: |
Gene expression patterns of in vivo-derived sheep blastocysts is more affected by vitrification than slow freezing technique. |
|
Ano de publicação: |
2020 |
|
Fonte/Imprenta: |
Cryobiology, v. 95, p. 110-115, 2020. |
|
DOI: |
https://doi.org/10.1016/j.cryobiol.2020.05.009 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Transfer of fresh sheep embryos frequently results in higher pregnancy rate compared to cryopreserved ones, possibly due to a failure in the communication between the cryopreserved embryo and the endometrium during pre-implantation and pregnancy establishment. Thus, this study assessed the effect of sheep embryo cryopres-ervation (slow freezing or vitrification) on embryo survival rate and expression of genes related to trophectoderm differentiation (CDX2), pluripotency maintenance (NANOG), cell proliferation (TGFB1), mitochondrial activity (NRF1) and apoptosis (BAX and BCL2). Superovulation (n ¼32 ewes) was performed and embryos were transcervically collected. One hundred good quality (Grade I and II) embryos were allocated into three groups: fresh embryos (CTL; n ¼15), slow freezing (SF; n ¼42) or vitrification (VT; n ¼43). After thawing/warming, three pools of five blastocysts per group were used for RT-qPCR; the remaining 55 embryos were cultured in vitro in SOFaa medium at 38.5 �C and 5% CO2 (SF: n ¼27 and VT: n ¼28). Survival rate of SF and VT were, respectively, 29.6% (8/27) and 14.2% (4/28) at 24 h; and 48.1% (13/27) and 32.1% (9/28) at 48 h (P >0.05). Only CDX2 was affected (up-regulated, P <0.05) in both groups compared to CTL. The BAX transcript was upregulated in VT, compared to SF group. The VT increased (P <0.05) the expression of all genes, except for NANOG and NRF1, when compared to the CTL. In conclusion, although in vitro survival was similar between techniques, VT led to increased changes in blastocyst gene expression compared to CTL and SF. MenosTransfer of fresh sheep embryos frequently results in higher pregnancy rate compared to cryopreserved ones, possibly due to a failure in the communication between the cryopreserved embryo and the endometrium during pre-implantation and pregnancy establishment. Thus, this study assessed the effect of sheep embryo cryopres-ervation (slow freezing or vitrification) on embryo survival rate and expression of genes related to trophectoderm differentiation (CDX2), pluripotency maintenance (NANOG), cell proliferation (TGFB1), mitochondrial activity (NRF1) and apoptosis (BAX and BCL2). Superovulation (n ¼32 ewes) was performed and embryos were transcervically collected. One hundred good quality (Grade I and II) embryos were allocated into three groups: fresh embryos (CTL; n ¼15), slow freezing (SF; n ¼42) or vitrification (VT; n ¼43). After thawing/warming, three pools of five blastocysts per group were used for RT-qPCR; the remaining 55 embryos were cultured in vitro in SOFaa medium at 38.5 �C and 5% CO2 (SF: n ¼27 and VT: n ¼28). Survival rate of SF and VT were, respectively, 29.6% (8/27) and 14.2% (4/28) at 24 h; and 48.1% (13/27) and 32.1% (9/28) at 48 h (P >0.05). Only CDX2 was affected (up-regulated, P <0.05) in both groups compared to CTL. The BAX transcript was upregulated in VT, compared to SF group. The VT increased (P <0.05) the expression of all genes, except for NANOG and NRF1, when compared to the CTL. In conclusion, although in vitro survival was similar between... Mostrar Tudo |
|
Palavras-Chave: |
Apoptosis regulator; Embryo cryopreservation; Embryonic metabolism; Reproductive biotechnologies. |
|
Thesaurus Nal: |
Embryo implantation; Embryo transfer; Ewes; Gene expression; Sheep. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 02656naa a2200337 a 4500
001 2124160
005 2025-01-10
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.cryobiol.2020.05.009$2DOI
100 1 $aBRAIR, V. L.
245 $aGene expression patterns of in vivo-derived sheep blastocysts is more affected by vitrification than slow freezing technique.$h[electronic resource]
260 $c2020
520 $aTransfer of fresh sheep embryos frequently results in higher pregnancy rate compared to cryopreserved ones, possibly due to a failure in the communication between the cryopreserved embryo and the endometrium during pre-implantation and pregnancy establishment. Thus, this study assessed the effect of sheep embryo cryopres-ervation (slow freezing or vitrification) on embryo survival rate and expression of genes related to trophectoderm differentiation (CDX2), pluripotency maintenance (NANOG), cell proliferation (TGFB1), mitochondrial activity (NRF1) and apoptosis (BAX and BCL2). Superovulation (n ¼32 ewes) was performed and embryos were transcervically collected. One hundred good quality (Grade I and II) embryos were allocated into three groups: fresh embryos (CTL; n ¼15), slow freezing (SF; n ¼42) or vitrification (VT; n ¼43). After thawing/warming, three pools of five blastocysts per group were used for RT-qPCR; the remaining 55 embryos were cultured in vitro in SOFaa medium at 38.5 �C and 5% CO2 (SF: n ¼27 and VT: n ¼28). Survival rate of SF and VT were, respectively, 29.6% (8/27) and 14.2% (4/28) at 24 h; and 48.1% (13/27) and 32.1% (9/28) at 48 h (P >0.05). Only CDX2 was affected (up-regulated, P <0.05) in both groups compared to CTL. The BAX transcript was upregulated in VT, compared to SF group. The VT increased (P <0.05) the expression of all genes, except for NANOG and NRF1, when compared to the CTL. In conclusion, although in vitro survival was similar between techniques, VT led to increased changes in blastocyst gene expression compared to CTL and SF.
650 $aEmbryo implantation
650 $aEmbryo transfer
650 $aEwes
650 $aGene expression
650 $aSheep
653 $aApoptosis regulator
653 $aEmbryo cryopreservation
653 $aEmbryonic metabolism
653 $aReproductive biotechnologies
700 1 $aMAIA, A. L. R. S.
700 1 $aCORREIA, L. F. L.
700 1 $aBARBOSA, N. O.
700 1 $aSANTOS, J. D. R.
700 1 $aBRANDÃO, F. Z.
700 1 $aFONSECA, J. F. da
700 1 $aBATISTA, R. I. T. P.
700 1 $aSOUZA-FABJAN, J. M. G.
773 $tCryobiology$gv. 95, p. 110-115, 2020.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 2 | |
| 1. |  | MARQUES, K. M.; MIGUEL, A. C. A.; DURIGAN, M. F. B.; GALATI, V. C.; DURIGAN, J. F. Associação da radiação ultravioleta e de revestimentos na conservação de mangas "palmer" previamente armazenadas sob condição promotora de injúria. In: SIMPÓSIO BRASILEIRO DE PÓS-COLHEITA DE FRUTAS, HORTALIÇAS E FLORES, 4., 2013, Ribeirão Preto. Anais... Ribeirão Preto, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Roraima. |
|    |
| 2. | | DURIGAN, M. F. B.; MIGUEL, A. C. A.; MARQUES, K. M.; MORGADO, C. M. A.; DURIGAN, J. F. Efeito da radiação ultravioleta (uv-c) e do uso de revestimentos na atividade antioxidante de mangas "palmer" armazenadas a 5 ºc, depois de transferidas à condição de ambiente. In: SIMPÓSIO BRASILEIRO DE PÓS-COLHEITA DE FRUTAS, HORTALIÇAS E FLORES, 4., 2013, Ribeirão Preto. Anais... Ribeirão Preto, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Roraima. |
| |
| Registros recuperados : 2 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
