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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
08/06/2020 |
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Data da última atualização: |
12/06/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CAMPESTRINI, L. H.; RASERA, G. B.; CAMARGO, A. C. de; FRANCHIN, M.; NANI, B. D.; ROSALEN, P. L.; CANNIATTI-BRAZACA, S. G.; BIASOTO, A. C. T.; SHAHIDIC, F.; ALENCAR, S. M. |
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Afiliação: |
Luciano Henrique Campestrini; Gabriela Boscariol Rasera; Adriano Costa de Camargo; Marcelo Franchin; Bruno Dias Nani; Pedro Luiz Rosalen; Solange Guidolin Canniatti-Brazaca; ALINE TELLES BIASOTO MARQUES, CPATSA; Fereidoon Shahidic; Severino Matias Alencar. |
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Título: |
Alkaline conditions better extract anti-inflammatory polysaccharides from winemaking by-products. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Food Research International, v. 131, may 2020. |
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DOI: |
https://doi.org/10.1016/j.foodres.2019.108532 |
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Idioma: |
Inglês |
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Conteúdo: |
Winemaking generates large amounts of by-products, a well recognized source of phenolic compounds. However, less attention has been paid to the polysaccharide-rich fraction (PRF) and effects of fractionation techniques on its potential bioactivity. Therefore, PRFs from Syrah and Tempranillo winemaking by-products were extracted under aqueous (neutral pH conditions), acidic and alkaline conditions. PRFs were screened for their monosaccharide composition, uronic acid content, homogeneity and molecular weight. Anti-inflammatory activity of PRFs were evaluated on stimulated RAW 264.7 macrophages. PRF obtained in water and/or under acidic conditions showed heterogeneous profiles. As like as in the others, a heterogeneous and complex profile was detected in extracts procured under alkaline conditions. A high content of uronic acid was found in aqueous extracts, thus indicating the presence of pectin. Pectin and hemicellulose were present in PRFs procured under acidic conditions. Alkaline conditions rendered extracts containing a complex mixture of monosaccharides, mainly xylose. This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 ?g/mL) by reducing the release of TNF-? and activation of NF-?B in LPS-activated RAW 264.7 macrophages, with no effect on cell viability. Regardless of the grape variety, PRFs obtained under alkaline conditions were the best option to obtain bioactive polysaccharides with potential application as a source of anti-inflammatory compounds. A complex mixture of polymers may be responsible for the anti-inflammatory effects. Finally, according to results procured by NMR, it is possible to suggest that bioactive fractions are composed of a chain of ?-L-Araf-(1 ? 3) linked, ?-D-Xylp- (1 ? 4), ?-D-Glcp-(1 ? 4) linked, ?-D-GalpA-(1 ? 4), ?-D-Gal-(1 ? 2) forming possible RG I and RG II and xylan chains. MenosWinemaking generates large amounts of by-products, a well recognized source of phenolic compounds. However, less attention has been paid to the polysaccharide-rich fraction (PRF) and effects of fractionation techniques on its potential bioactivity. Therefore, PRFs from Syrah and Tempranillo winemaking by-products were extracted under aqueous (neutral pH conditions), acidic and alkaline conditions. PRFs were screened for their monosaccharide composition, uronic acid content, homogeneity and molecular weight. Anti-inflammatory activity of PRFs were evaluated on stimulated RAW 264.7 macrophages. PRF obtained in water and/or under acidic conditions showed heterogeneous profiles. As like as in the others, a heterogeneous and complex profile was detected in extracts procured under alkaline conditions. A high content of uronic acid was found in aqueous extracts, thus indicating the presence of pectin. Pectin and hemicellulose were present in PRFs procured under acidic conditions. Alkaline conditions rendered extracts containing a complex mixture of monosaccharides, mainly xylose. This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 ?g/mL) by reducing the release of TNF-? and activation of NF-?B in LPS-activated RAW 264.7 macrophages, with no effect on cell viability. Regardless of the grape variety, PRFs obtained under alkaline conditions were the best option to obtain bioactive polysaccharides with potential application as a source of anti-inflammatory c... Mostrar Tudo |
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Palavras-Chave: |
Atividade anti-inflamatória; Extração sequencial; Polissacarídeos; Vinificação. |
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Thesagro: |
Pectina; Subproduto; Uva. |
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Thesaurus Nal: |
Grapes; Polysaccharides. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02892naa a2200349 a 4500
001 2123132
005 2020-06-12
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.foodres.2019.108532$2DOI
100 1 $aCAMPESTRINI, L. H.
245 $aAlkaline conditions better extract anti-inflammatory polysaccharides from winemaking by-products.$h[electronic resource]
260 $c2020
520 $aWinemaking generates large amounts of by-products, a well recognized source of phenolic compounds. However, less attention has been paid to the polysaccharide-rich fraction (PRF) and effects of fractionation techniques on its potential bioactivity. Therefore, PRFs from Syrah and Tempranillo winemaking by-products were extracted under aqueous (neutral pH conditions), acidic and alkaline conditions. PRFs were screened for their monosaccharide composition, uronic acid content, homogeneity and molecular weight. Anti-inflammatory activity of PRFs were evaluated on stimulated RAW 264.7 macrophages. PRF obtained in water and/or under acidic conditions showed heterogeneous profiles. As like as in the others, a heterogeneous and complex profile was detected in extracts procured under alkaline conditions. A high content of uronic acid was found in aqueous extracts, thus indicating the presence of pectin. Pectin and hemicellulose were present in PRFs procured under acidic conditions. Alkaline conditions rendered extracts containing a complex mixture of monosaccharides, mainly xylose. This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 ?g/mL) by reducing the release of TNF-? and activation of NF-?B in LPS-activated RAW 264.7 macrophages, with no effect on cell viability. Regardless of the grape variety, PRFs obtained under alkaline conditions were the best option to obtain bioactive polysaccharides with potential application as a source of anti-inflammatory compounds. A complex mixture of polymers may be responsible for the anti-inflammatory effects. Finally, according to results procured by NMR, it is possible to suggest that bioactive fractions are composed of a chain of ?-L-Araf-(1 ? 3) linked, ?-D-Xylp- (1 ? 4), ?-D-Glcp-(1 ? 4) linked, ?-D-GalpA-(1 ? 4), ?-D-Gal-(1 ? 2) forming possible RG I and RG II and xylan chains.
650 $aGrapes
650 $aPolysaccharides
650 $aPectina
650 $aSubproduto
650 $aUva
653 $aAtividade anti-inflamatória
653 $aExtração sequencial
653 $aPolissacarídeos
653 $aVinificação
700 1 $aRASERA, G. B.
700 1 $aCAMARGO, A. C. de
700 1 $aFRANCHIN, M.
700 1 $aNANI, B. D.
700 1 $aROSALEN, P. L.
700 1 $aCANNIATTI-BRAZACA, S. G.
700 1 $aBIASOTO, A. C. T.
700 1 $aSHAHIDIC, F.
700 1 $aALENCAR, S. M.
773 $tFood Research International$gv. 131, may 2020.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Biblioteca |
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Tipo/Formato |
Classificação |
Cutter |
Registro |
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| Registros recuperados : 23 | |
| 6. |  | SCHACHT, W. H.; KAWAS, J. R.; MALECHEK, J. C.; FREIRE, L. C. L. Efeito da suplementação de energia e nitrogênio no ganho de peso de caprinos em pastagem nativa (caatinga) na época seca, no nordeste do Brasil. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 22., 1985, Balneário Camboriu, SC. Anais... Balneário Camboriú: Sociedade Brasileira de Zootecnia, 1985. p. 62.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 7. | | SILVA, F. L. R. da; ARRUDA, F. de A. V.; KAWAS, J. R. Efeito do sombreamento sobre o consumo de alimento e água, em ovinos da raça Santa Inês Vermelha do Nordeste. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 25., 1988, Viçosa, MG. Anais... Viçosa, MG: Sociedade Brasileira de Zootecnia, 1988. p. 302.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 8. | | BARROS, N. N.; KAWAS, J. R.; LOPES, E. A.; JOHNSON, W. L. Estudo comparativo da digestibilidade de leguminosa nativa com caprinos e ovinos, no semi-árido do Estado do Ceará. II. Digestibilidade in vivo da silagem de mata-pasto (Cassia ssp). Pesquisa Agropecuária Brasileira, Brasília, DF, v. 27, n. 11, p. 1551-1555, nov., 1992.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 10. | | KAWAS, J. R.; GUIMARAES, W.; BARROS, N. N.; SANCHEZ, O.; SHELTON, J. M.; JOHNSON, W. L. Interrelação energia-proteína no crescimento de ovinos deslanados. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 23., 1986, Campo Grande. Anais... Campo Grande: Sociedade Brasileira de Zootecnia, 1986. p. 95.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 11. | | BARROS, N. N.; KAWAS, J. R.; JOHNSON, W. L.; SHELTON, J. M.; SOUZA NETO, J. de. Influência da energia do desempenho de cordeiros Somalis alimentados com capim elefante (Pennisetum purpureum). In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 25., 1988, Viçosa, MG. Anais... Viçosa, MG: Sociedade Brasileira de Zootecnia, 1988. p. 114.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 12. | | KAWAS, J. R.; LOPES, J.; DANELON, D. L.; LU, C. D. Influence of forage-to-concentrate ratios on intake, digestibility, chewing and milk production of dairy goats. Small Ruminant Research, v. 4, n. 1, p. 11-18, 1991.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 14. | | LU, C. D.; OSBORN, R. G.; KAWAS, J. R.; ARRUDA, F. Prediction of dry matter intake in lactating and growing dairy goats. In: INTERNATIONAL CONFERENCE ON GOATS, 4., 1987, Brasília, DF. Proceedings... Brasília, DF: EMBRAPA-DDT, 1987. p. 1386. Resumo 121.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 15. | | KAWAS, J. R.; GUIMARAES, W.; BARROS, N. N.; FREIRE, L. C. L.; FOOTE, W. C. Survival and growth of goat kids grazing native range from dams supplemented with incremental energy levels. In: INTERNATIONAL CONFERENCE ON GOATS, 4., 1987, Brasília, DF. Proceedings... Brasília, DF: EMBRAPA-DDT, 1987. p. 1399. Abstract 139.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 16. | | BARROS, N. N.; OLIVEIRA, E. R.; ROBB, T. W.; ARRUDA, F. A. V.; KAWAS, J. R.; JOHNSON, W. L. Acabamento de caprinos e ovinos durante a época seca, no Nordeste. In: REUNIÃO TÉCNICO-CIENTÍFICA DO PROGRAMA DE APOIO A PESQUISA COLABORATIVA DE PEQUENOS RUMINANTES, 1., 1986. Sobral. Anais... Sobral: EMBRAPA-CNPC; SR-CRSP, 1986. p. 285-295. (EMBRAPA-CNPC. Documentos, 6).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 17. | | BARROS, N. N.; KAWAS, J. R.; FREIRE, L. C. L.; JOHNSON, W. L.; SANTOS, J. W. dos. Nutritive value of total mixed rations with various energy sources fed to lambs, in Northeast Brazil. Journal of Animal Science, v. 61, suppl.1, p. 452, 1985. Abstract 592. Abstracts of the 77 ASAS Annual Meeting, Biloxi, Mississipi, 1985.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 18. | | ARRUDA, F. A. V.; BEZERRA, R.; RIBEIRO, F. L.; SANTOS, J. W. dos; KAWAS, J. R.; SHELTON, J. M. The interaction of environmental variables (time of day and shade vs sun) and level of nutrition on animal response (body temperature, respiratory rate, water intake and weight gains) with hair sheep. In: REUNIÃO TÉCNICO-CIENTÍFICA DO PROGRAMA DE APOIO À PESQUISA COLABORATIVA DE PEQUENOS RUMINANTES, 1., 1986, Sobral. Caprinos e ovinos no Nordeste do Brasil: anais. Sobral: EMBRAPA-CNPC: SR-CRSP, 1986. p. 259-265. (EMBRAPA-CNPC. Documentos, 6).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 19. | | KAWAS, J. R.; CARNEIRO, H.; BARROS, N. N.; FREIRE, L. C. L.; KAWAS, F. N.; SHELTON, M.; JOHNSON, W. L. Valor nutritivo para caprinos das silagens de sorgo forrageiro (Sorghum vulgare) e de cunhã (Clitoria ternatea) em dois estágios de maturidade. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 22., 1985, Balneário Camboriú, SC. Anais... Balneário Camboriú: Sociedade Brasileira de Zootecnia, 1985. p. 355.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 20. | | BARROS, N. N.; KAWAS, J. R.; FREIRE, L. C. L.; ARAUJO FILHO, J. A. de; SHELTON, J. M.; JOHNSON, W. L. Digestibility and intake of various native and introduced forages by goats and hair sheep in Northeast Brazil. In: REUNIÃO TÉCNICO-CIENTÍFICA DO PROGRAMA DE APOIO A PESQUISA COLABORATIVA DE PEQUENOS RUMINANTES, 1., 1986. Sobral. Anais... Sobral: EMBRAPA-CNPC: SR-CRSP, 1986. p. 219-233. (EMBRAPA-CNPC. Documentos, 6).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 23 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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