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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
11/12/2019 |
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Data da última atualização: |
11/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PIZAURO, L. J. L.; ALMEIDA, C. C. de; SOLTES, G. A.; SLAVIC, D.; ÁVILA, F. A. de; ZAFALON, L. F.; MACINNES, J. I. |
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Afiliação: |
Lucas J. L. Pizauro, UNESP; Camila C. de Almeida, UNESP; Glenn A. Soltes, University of Guelph; Durda Slavic, University of Guelph; Fernando A. de Ávila, UNESP; LUIZ FRANCISCO ZAFALON, CPPSE; Janet I. MacInnes, University of Guelph. |
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Título: |
Detection of antibiotic resistance, mecA, and virulence genes in coagulase-negative Staphylococcus spp. from buffalo milk and the milking environment. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Journal of Dairy Science, v.102, n.12, dec. 2019. |
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Páginas: |
p.11459-11464 |
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DOI: |
https://doi.org/10.3168/jds.2018-15920 |
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Idioma: |
Inglês |
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Notas: |
Short communication. |
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Conteúdo: |
The aim of this study was to determinate whether coagulase-negative staphylococci (CNS) from buffalo milk or the milking environment possess virulence factors that are associated with intramammary infections or antimicrobial resistance. Milk samples (n = 320) from 80 lactating buffalo were evaluated for clinical and subclinical mastitis by physical examination, the strip cup test, California Mastitis Test (CMT), and somatic cell count (SCC) over a 4-mo period. In addition, swabs were obtained from the hands of consenting milkers (16), liners (64), and from the mouths (15) and nostrils (15) of buffalo calves. No clinical cases of mastitis were observed; however, CMT together with SCC results indicated that 8 animals had subclinical mastitis. Eighty-four CNS isolates were identified by MALDI-TOF MS and cydB real-time PCR (qPCR) and then evaluated by qPCR for presence of the eta, etb, sea, sec, cna, seb, sei, seq, sem, seg, see, and tst toxin genes, adhesion- and biofilm-associated genes (eno, ebps, fib, fnbA, coa), and the methicillin resistance gene (mecA). Resistance to antibiotics commonly used for mastitis treatment in Brazil was determined using the Kirby-Bauer test. Two strains were positive for the see and eta toxin genes; and mecA (1), eno (27), ebps (10), fnbA (10), and coa (5) genes were also detected. A notable number of isolates were resistant to erythromycin (30), penicillin (26), and cotrimoxazole (18); importantly, 10 vancomycin-resistant isolates were also detected. A smaller number of isolates were resistant to rifampicin (8), oxacillin (7), clindamycin (5), cefepime (4), tetracycline (3), ciprofloxacin (2), and chloramphenicol (1), and none were resistant to gentamicin or ciprofloxacin. Isolates with resistance to 2 (13 isolates), 3 (3), 4 (3), 5 (1), and 6 (1) antibiotics were detected. Taken together, our findings suggest that CNS isolates may not be a significant cause of clinical or even subclinical mastitis in buffaloes, but they may be a reservoir of virulence and antibiotic resistance genes. MenosThe aim of this study was to determinate whether coagulase-negative staphylococci (CNS) from buffalo milk or the milking environment possess virulence factors that are associated with intramammary infections or antimicrobial resistance. Milk samples (n = 320) from 80 lactating buffalo were evaluated for clinical and subclinical mastitis by physical examination, the strip cup test, California Mastitis Test (CMT), and somatic cell count (SCC) over a 4-mo period. In addition, swabs were obtained from the hands of consenting milkers (16), liners (64), and from the mouths (15) and nostrils (15) of buffalo calves. No clinical cases of mastitis were observed; however, CMT together with SCC results indicated that 8 animals had subclinical mastitis. Eighty-four CNS isolates were identified by MALDI-TOF MS and cydB real-time PCR (qPCR) and then evaluated by qPCR for presence of the eta, etb, sea, sec, cna, seb, sei, seq, sem, seg, see, and tst toxin genes, adhesion- and biofilm-associated genes (eno, ebps, fib, fnbA, coa), and the methicillin resistance gene (mecA). Resistance to antibiotics commonly used for mastitis treatment in Brazil was determined using the Kirby-Bauer test. Two strains were positive for the see and eta toxin genes; and mecA (1), eno (27), ebps (10), fnbA (10), and coa (5) genes were also detected. A notable number of isolates were resistant to erythromycin (30), penicillin (26), and cotrimoxazole (18); importantly, 10 vancomycin-resistant isolates were also dete... Mostrar Tudo |
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Palavras-Chave: |
Dairy buffalo; Virulence genes. |
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Thesaurus Nal: |
Antibiotic resistance; Biofilm. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02937naa a2200277 a 4500
001 2116604
005 2019-12-11
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3168/jds.2018-15920$2DOI
100 1 $aPIZAURO, L. J. L.
245 $aDetection of antibiotic resistance, mecA, and virulence genes in coagulase-negative Staphylococcus spp. from buffalo milk and the milking environment.$h[electronic resource]
260 $c2019
300 $ap.11459-11464
500 $aShort communication.
520 $aThe aim of this study was to determinate whether coagulase-negative staphylococci (CNS) from buffalo milk or the milking environment possess virulence factors that are associated with intramammary infections or antimicrobial resistance. Milk samples (n = 320) from 80 lactating buffalo were evaluated for clinical and subclinical mastitis by physical examination, the strip cup test, California Mastitis Test (CMT), and somatic cell count (SCC) over a 4-mo period. In addition, swabs were obtained from the hands of consenting milkers (16), liners (64), and from the mouths (15) and nostrils (15) of buffalo calves. No clinical cases of mastitis were observed; however, CMT together with SCC results indicated that 8 animals had subclinical mastitis. Eighty-four CNS isolates were identified by MALDI-TOF MS and cydB real-time PCR (qPCR) and then evaluated by qPCR for presence of the eta, etb, sea, sec, cna, seb, sei, seq, sem, seg, see, and tst toxin genes, adhesion- and biofilm-associated genes (eno, ebps, fib, fnbA, coa), and the methicillin resistance gene (mecA). Resistance to antibiotics commonly used for mastitis treatment in Brazil was determined using the Kirby-Bauer test. Two strains were positive for the see and eta toxin genes; and mecA (1), eno (27), ebps (10), fnbA (10), and coa (5) genes were also detected. A notable number of isolates were resistant to erythromycin (30), penicillin (26), and cotrimoxazole (18); importantly, 10 vancomycin-resistant isolates were also detected. A smaller number of isolates were resistant to rifampicin (8), oxacillin (7), clindamycin (5), cefepime (4), tetracycline (3), ciprofloxacin (2), and chloramphenicol (1), and none were resistant to gentamicin or ciprofloxacin. Isolates with resistance to 2 (13 isolates), 3 (3), 4 (3), 5 (1), and 6 (1) antibiotics were detected. Taken together, our findings suggest that CNS isolates may not be a significant cause of clinical or even subclinical mastitis in buffaloes, but they may be a reservoir of virulence and antibiotic resistance genes.
650 $aAntibiotic resistance
650 $aBiofilm
653 $aDairy buffalo
653 $aVirulence genes
700 1 $aALMEIDA, C. C. de
700 1 $aSOLTES, G. A.
700 1 $aSLAVIC, D.
700 1 $aÁVILA, F. A. de
700 1 $aZAFALON, L. F.
700 1 $aMACINNES, J. I.
773 $tJournal of Dairy Science$gv.102, n.12, dec. 2019.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| Registros recuperados : 16 | |
| 5. |  | JUNIOR, C.; BLANCO, J.; MOREIRA, R.; CRUZ, T.; MASSON, L.; SILVA, J. Avaliação da atividade antioxidante e antimicrobiana de óleo essencial de orégano em maionese. In: JORNADA INTERNA DE INICIAÇÃO CIENTÍFICA E TECNOLÓGICA, 8., 2014, Rio de Janeiro. Anais... Rio de Janeiro: IFRJ, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 8. | | ROCHA, A.; ROSENTHAL, A.; FERREIRA, E.; MASSON, L.; CAMARGO, L.; MASSAGUER, P. Validation of thermal inactivation processing of the most heat resistance mould in commercial passion fruit juice. In: WORLD CONGRESS OF FOOD SCIENCE & TECHNOLOGY, 13., 2006, Nates, France. Abstracts... Nates: [s. n.], 2006. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 9. | | MATHIAS, S. P.; ROSENTHAL, A.; GASPAR, A.; DELIZA, R.; SLONGO, A. P.; VICENTE, J.; MASSON, L. M.; BARBOSA, C. Alterações oxidativas (cor e lipídios) em presunto de peru tratado por Alta Pressão Hidrostática (APH). Ciência e Tecnologia de Alimentos, Campinas, v. 30, n. 4, p. 852-857, out./dez. 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 11. | | FERREIRA, E. H. R.; ROSENTHAL, A.; XAVIER, A.; MASSON, L. M. P.; ROCHA, A. C.; MASSAGUER, P. R. Seleção e caracterização de fungos isolados de maracujá envasado em linha de processamento asséptico. In.: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DE ALIMENTOS, 5., 2003, Campinas, SP. Resumos... Campinas, 2003. 1 CD-Rom.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 12. | | GOMES, P. S.; LOPES, R. F. R.; AGUIAR, L. B.; ALMEIDA, L. T. G.; MASSON, L. M. P.; SILVA, J. P. L. da. Avaliação da atividade antioxidante de óleo essencial de orégano em maionese. In: SEMINÁRIO EM INOVAÇÃO E TECNOLOGIA NA ÁREA DE ALIMENTOS, 3., 2014, Rio de Janeiro. Resumos... Rio de Janeiro: IFRJ, 2014. 1 CD-ROM. Ref. SITA 029| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 13. | | LOPES, J. F. R.; AGUIAR, L. B.; TORRES, L. G. A.; GOMES, P. S.; SILVA, J. P. L. da; MASSON, L. M. P. Avaliação da atividade antioxidante de óleo essencial de orégano em maionese. In: JORNADA INTERNA DE INICIAÇÃO CIENTÍFICA E TECNOLÓGICA, 9.; FÓRUM DE INOVAÇÃO, TECNOLOGIA E EDUCAÇÃO, 4., 2015, Rio de Janeiro. Rio de Janeiro: IFRJ, 2015. Trabalho CA-07. 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 14. | | GOMES, I. A.; LINDENBLATT, C. T.; MASSON, L. M. P.; GOMES, F. dos S.; FREITAS-SILVA, O.; SILVA, J. P. L. da. Effect Of Oregano Essential Oil On Oxidative Stability Of Low-Acid Mayonnaise. IOSR Journal Of Pharmacy, v. 6, n. 11, p. 45-52, nov. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 15. | | FERREIRA, E. H. da R.; MASSON, L. M. P; ROSENTHAL, A.; SOUZA, M. de L.; TASHIMA, L.; MASSAGUER, P. R. de. Termorresistência de fungos filamentosos isolados de néctares de frutas envasados assepticamente. Brazilian Journal of Food Technology, Campinas, v. 14, n. 3, p. 164-171, jul./set. 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 16. | | GOMES, I. A.; LINDENBLATT, C. T.; MASSON, L. M. P.; GOMES, F. dos S.; FREITAS-SILVA, O.; SILVA, J. P. L. da. Utilização de óleo essencial de orégano como antimicrobiano e antioxidante natural em maionese de baixa acidez. In: SIMPÓSIO DE ALIMENTOS E NUTRIÇÃO, 2., 2015, Rio de Janeiro. O Semear da ciência: caderno de resumos. Rio de Janeiro: UNIRIO, 2015. Ref. AF003. 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| Registros recuperados : 16 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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