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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
20/11/2019 |
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Data da última atualização: |
31/01/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PAIVA, B. A. R. de; WENDLAND, A.; TEIXEIRA, N. C.; FERREIRA, M. A. S. V. |
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Afiliação: |
BRUNA ALICIA RAFAEL DE PAIVA, UNIVERSIDADE DE BRASÍLIA; ADRIANE WENDLAND FERREIRA, CNPAF; NARA CRISTINA TEIXEIRA; MARISA A. S. V. FERREIRA, UNIVERSIDADE DE BRASÍLIA. |
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Título: |
Rapid detection of Xanthomonas citri pv. fuscans and Xanthomonas phaseoli pv. phaseoli in common bean by loop-mediated isothermal amplification. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Plant Disease, v. 104, n. 1, p. 198-203, Jan. 2020. |
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ISSN: |
0191-2917 |
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DOI: |
https://doi.org/10.1094/PDIS-02-19-0325-RE |
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Idioma: |
Inglês |
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Conteúdo: |
A single loop-mediated isothermal amplification (LAMP) assay was developed for specific detection of both pathogens that cause bacterial blight in common bean, Xanthomonas phaseoli pv. phaseoli (Xpp) and Xanthomonas citri pv. fuscans (Xcf). The objective was to provide a simple, easy-to-use, specific, and sensitive method to investigate the presence of one or both pathogens in plant material and seeds for routine diagnosis. The detection limits for both pathogens were 10 CFU/ml for cell suspensions and 1 fg of DNA, whereas in conventional PCR, the primers detected up to 105 CFU/ml and 1 ng of DNA. Specificity was confirmed by testing DNA from bean leaves, other Xanthomonas species, common fungal and bacterial bean pathogens, and bacteria from the leaf microbiota. The method was tested with bean leaves inoculated with Xpp, and the pathogen could be detected from 4 h up to 15 days postinoculation, even before disease symptoms were visible. When the method was applied to bacterium detection (Xpp or Xcf) in seed lots from infected plants, the bacterium detection rate was 100% (24 of 24). The pathogens were detected in seeds incubated for just 1 h in saline solution (0.85%), reducing the time needed for bacterium detection. The LAMP assay could be useful as a tool in bean bacterial blight management. Rapid and sensitive detection of bacteria in bean seed lots would reduce the risks of planting highly contaminated seeds in environments favorable to blight multiplication. |
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Thesagro: |
Bactéria; Doença de Planta; Feijão; Phaseolus Vulgaris; Xanthomonas Phaseoli. |
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Thesaurus Nal: |
Bacterial diseases of plants; Beans; Fields; Loop-mediated isothermal amplification; Oilseeds; Pathogen identification; Plant diseases and disorders. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02536naa a2200325 a 4500
001 2114711
005 2025-01-31
008 2020 bl uuuu u00u1 u #d
022 $a0191-2917
024 7 $ahttps://doi.org/10.1094/PDIS-02-19-0325-RE$2DOI
100 1 $aPAIVA, B. A. R. de
245 $aRapid detection of Xanthomonas citri pv. fuscans and Xanthomonas phaseoli pv. phaseoli in common bean by loop-mediated isothermal amplification.$h[electronic resource]
260 $c2020
520 $aA single loop-mediated isothermal amplification (LAMP) assay was developed for specific detection of both pathogens that cause bacterial blight in common bean, Xanthomonas phaseoli pv. phaseoli (Xpp) and Xanthomonas citri pv. fuscans (Xcf). The objective was to provide a simple, easy-to-use, specific, and sensitive method to investigate the presence of one or both pathogens in plant material and seeds for routine diagnosis. The detection limits for both pathogens were 10 CFU/ml for cell suspensions and 1 fg of DNA, whereas in conventional PCR, the primers detected up to 105 CFU/ml and 1 ng of DNA. Specificity was confirmed by testing DNA from bean leaves, other Xanthomonas species, common fungal and bacterial bean pathogens, and bacteria from the leaf microbiota. The method was tested with bean leaves inoculated with Xpp, and the pathogen could be detected from 4 h up to 15 days postinoculation, even before disease symptoms were visible. When the method was applied to bacterium detection (Xpp or Xcf) in seed lots from infected plants, the bacterium detection rate was 100% (24 of 24). The pathogens were detected in seeds incubated for just 1 h in saline solution (0.85%), reducing the time needed for bacterium detection. The LAMP assay could be useful as a tool in bean bacterial blight management. Rapid and sensitive detection of bacteria in bean seed lots would reduce the risks of planting highly contaminated seeds in environments favorable to blight multiplication.
650 $aBacterial diseases of plants
650 $aBeans
650 $aFields
650 $aLoop-mediated isothermal amplification
650 $aOilseeds
650 $aPathogen identification
650 $aPlant diseases and disorders
650 $aBactéria
650 $aDoença de Planta
650 $aFeijão
650 $aPhaseolus Vulgaris
650 $aXanthomonas Phaseoli
700 1 $aWENDLAND, A.
700 1 $aTEIXEIRA, N. C.
700 1 $aFERREIRA, M. A. S. V.
773 $tPlant Disease$gv. 104, n. 1, p. 198-203, Jan. 2020.
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Embrapa Arroz e Feijão (CNPAF) |
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| 1. |  | SOARES, F. C.; ALMEIDA, J. C. de; KOGA, R. H.; DEL DUQUE, D. M. S.; SILVA, G. T. S. T. da; RIBEIRO, C.; MENDONÇA, V. R. de. TiO2/BiVO4 composite from preformed nanoparticles for heterogeneous photocatalysis. Materials Chemistry and Physics, v. 290, e126588, 2022. 1 - 10| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Instrumentação. |
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| 2. | | ALMEIDA, J. C.; CORRÊA, M. T.; KOGA, R. H.; DEL DUQUE, D. M. S.; LOPES, O. F.; SILVA, G. T. S. T.; OLIVEIRA, C. R. de; MENDONÇA, V. R. Crystallization time in ZnO: the role of surface OH groups in its photoactivity. New Journal of Chemistry, v. 44, 2020. 18216-18224| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Instrumentação. |
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