| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
15/10/2019 |
|
Data da última atualização: |
22/10/2019 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
OLIVEIRA, S. A. S. de; FERREIRA, C. F.; DIAMANTINO, M. S. A. S.; SANTOS, T. A.; PEREIRA, J. dos S.; OLIVEIRA, E. J. de. |
|
Afiliação: |
SAULO ALVES SANTOS DE OLIVEIRA, CNPMF; CLAUDIA FORTES FERREIRA, CNPMF; MARIA SELMA ALVES SILVA DIAMANTINO; TAÍS ARAÚJO SANTOS, UFRB; JOCILENE DOS SANTOS PEREIRA, UFRB; EDER JORGE DE OLIVEIRA, CNPMF. |
|
Título: |
First report of cassava torrado-like virus, cassava polero-like virus and cassava new alphaflexivirus associated with cassava frogskin disease in Brazil. |
|
Ano de publicação: |
2019 |
|
Fonte/Imprenta: |
Journal of Plant Pathology, August, 2019. |
|
ISSN: |
1125-4653 |
|
DOI: |
https://doi.org/10.1007/s42161-019-00384-6 |
|
Idioma: |
Inglês |
|
Notas: |
Online first. |
|
Conteúdo: |
Cassava (Manihot esculenta Crantz) plants with typical symptoms of frogskin disease (CFSD) have been observed since 2012 in Cruz das Almas, Bahia, Brazil. The above ground parts of the plants were asymptomatic, however, the roots showed typical symptoms of woody-like appearance, thickened cork-like peel, opaque aspect, and coalescent lip-like slits in a honeycomb pattern. Plants did not test positive for phytoplasma based on a nested-PCR reaction for the 16S region (Alvarez et al. 2009). Therefore, we hypothesized a viral infection. To verify our hypothesis, total RNA was extracted from 0.5 g of plant tissue using CTAB followed by reverse transcription and polymerase chain reaction with specific primers for: (i) cassava frogskin-associated virus ? CsFSaV; (ii) ?cassava torrado-like virus? ? CsTLV; (iii) ?cassava polero-like virus? ? CsPLV and (iv) ?cassava new alphaflexivirus? ? CsNAV, as described by Carvajal-Yepes et al. (2014). Amplicons were obtained for CsTLV (RNA1: 834 bp, RNA2: 719 bp), CsNAV (1227 bp) and CsPLV (1001 bp). No amplicons were obtained for CsFSaV. PCR products were sequenced in both directions, manually edited and deposited in the NCBI database: CsTLV (MN194209, MN194210 and MN194211) and RNA 2 (MN194212, MN194213 and MN194214), CsPLV (MN172358, MN207078 and MN207079) CsNAV (MN207080, MN207081 and MN207082). A BLASTn analysis of the viral sequences showed 96?100% identity with CsTLV (RNA1 polyprotein: KC505250), CsTLV (RNA2 polyprotein: KC505251); CsPLV (RNA-dependent RNA polymerase and coat protein: KF885738) and CsNAV (replicase, TGB1, TGB2, and coat protein: KC505252 and KY288516), respectively (E-value <0.001). The three putative virus species (CsTLV, CsNAV and CsPLV) were described for the first time by Carvajal-Yepes et al. (2014) on association with CFSD together with CsFSaV in Colombia. To our knowledge this is the first report of these three viruses associated with CFSD in Brazil. MenosCassava (Manihot esculenta Crantz) plants with typical symptoms of frogskin disease (CFSD) have been observed since 2012 in Cruz das Almas, Bahia, Brazil. The above ground parts of the plants were asymptomatic, however, the roots showed typical symptoms of woody-like appearance, thickened cork-like peel, opaque aspect, and coalescent lip-like slits in a honeycomb pattern. Plants did not test positive for phytoplasma based on a nested-PCR reaction for the 16S region (Alvarez et al. 2009). Therefore, we hypothesized a viral infection. To verify our hypothesis, total RNA was extracted from 0.5 g of plant tissue using CTAB followed by reverse transcription and polymerase chain reaction with specific primers for: (i) cassava frogskin-associated virus ? CsFSaV; (ii) ?cassava torrado-like virus? ? CsTLV; (iii) ?cassava polero-like virus? ? CsPLV and (iv) ?cassava new alphaflexivirus? ? CsNAV, as described by Carvajal-Yepes et al. (2014). Amplicons were obtained for CsTLV (RNA1: 834 bp, RNA2: 719 bp), CsNAV (1227 bp) and CsPLV (1001 bp). No amplicons were obtained for CsFSaV. PCR products were sequenced in both directions, manually edited and deposited in the NCBI database: CsTLV (MN194209, MN194210 and MN194211) and RNA 2 (MN194212, MN194213 and MN194214), CsPLV (MN172358, MN207078 and MN207079) CsNAV (MN207080, MN207081 and MN207082). A BLASTn analysis of the viral sequences showed 96?100% identity with CsTLV (RNA1 polyprotein: KC505250), CsTLV (RNA2 polyprotein: KC505251); CsPLV ... Mostrar Tudo |
|
Thesagro: |
Mandioca. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02711naa a2200229 a 4500
001 2113112
005 2019-10-22
008 2019 bl uuuu u00u1 u #d
022 $a1125-4653
024 7 $ahttps://doi.org/10.1007/s42161-019-00384-6$2DOI
100 1 $aOLIVEIRA, S. A. S. de
245 $aFirst report of cassava torrado-like virus, cassava polero-like virus and cassava new alphaflexivirus associated with cassava frogskin disease in Brazil.$h[electronic resource]
260 $c2019
500 $aOnline first.
520 $aCassava (Manihot esculenta Crantz) plants with typical symptoms of frogskin disease (CFSD) have been observed since 2012 in Cruz das Almas, Bahia, Brazil. The above ground parts of the plants were asymptomatic, however, the roots showed typical symptoms of woody-like appearance, thickened cork-like peel, opaque aspect, and coalescent lip-like slits in a honeycomb pattern. Plants did not test positive for phytoplasma based on a nested-PCR reaction for the 16S region (Alvarez et al. 2009). Therefore, we hypothesized a viral infection. To verify our hypothesis, total RNA was extracted from 0.5 g of plant tissue using CTAB followed by reverse transcription and polymerase chain reaction with specific primers for: (i) cassava frogskin-associated virus ? CsFSaV; (ii) ?cassava torrado-like virus? ? CsTLV; (iii) ?cassava polero-like virus? ? CsPLV and (iv) ?cassava new alphaflexivirus? ? CsNAV, as described by Carvajal-Yepes et al. (2014). Amplicons were obtained for CsTLV (RNA1: 834 bp, RNA2: 719 bp), CsNAV (1227 bp) and CsPLV (1001 bp). No amplicons were obtained for CsFSaV. PCR products were sequenced in both directions, manually edited and deposited in the NCBI database: CsTLV (MN194209, MN194210 and MN194211) and RNA 2 (MN194212, MN194213 and MN194214), CsPLV (MN172358, MN207078 and MN207079) CsNAV (MN207080, MN207081 and MN207082). A BLASTn analysis of the viral sequences showed 96?100% identity with CsTLV (RNA1 polyprotein: KC505250), CsTLV (RNA2 polyprotein: KC505251); CsPLV (RNA-dependent RNA polymerase and coat protein: KF885738) and CsNAV (replicase, TGB1, TGB2, and coat protein: KC505252 and KY288516), respectively (E-value <0.001). The three putative virus species (CsTLV, CsNAV and CsPLV) were described for the first time by Carvajal-Yepes et al. (2014) on association with CFSD together with CsFSaV in Colombia. To our knowledge this is the first report of these three viruses associated with CFSD in Brazil.
650 $aMandioca
700 1 $aFERREIRA, C. F.
700 1 $aDIAMANTINO, M. S. A. S.
700 1 $aSANTOS, T. A.
700 1 $aPEREIRA, J. dos S.
700 1 $aOLIVEIRA, E. J. de
773 $tJournal of Plant Pathology, August, 2019.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Gado de Corte. |
|
Data corrente: |
19/05/2008 |
|
Data da última atualização: |
19/05/2008 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
SILVA, S. V. da; CAPRONI, A. L.; MIRANDA, C. H. B.; MACEDO, M. C. M.; FIGUEIRA, A. F. |
|
Afiliação: |
Simone Vieira da Silva UCBD bolsista PIBIC do CNPGC; A. L. Caproni, pesquisadora DCR do CNPq/FUNDECT; Cesar Heraclides Behling Miranda, CNPGC; Manuel Cláudio Motta Macedo, CNPGC; A. F. Figueira, doutoranda UFRRJ. |
|
Título: |
Fungos micorrízicos arbusculares em sistemas de integração lavoura e pecuária. |
|
Ano de publicação: |
2006 |
|
Fonte/Imprenta: |
In: JORNADA CIENTÍFICA DA EMBRAPA GADO DE CORTE, 2., 2006, Campo Grande, MS. Anais [da]... 2. ed. Campo Grande, MS: Embrapa Gado de Corte, 2006. |
|
Páginas: |
1 p. |
|
Descrição Física: |
1 CD-ROM. |
|
Idioma: |
Português |
|
Notas: |
CNPGC. |
|
Conteúdo: |
Fungos micorrízicos arbusculares (FMAs) são simbiontes que aumentam a área de exploração efetiva das raízes, facilitando absorção de nutrientes essenciais pelas plantas. As comunidades de FMAs de um dado ecossistema podem ser avaliadas pela extração das estruturas de resistência que formam (esporos), que são distintos em cada espécie. Avaliou-se a diversidade de FMAs presentes num solo sob sistemas contrastantes de integração lavoura-pecuária, com o objetivo de avaliar os efeitos da exploração agropecuária na diversidade destes fungos, comparadas a uma mata original da área. |
|
Palavras-Chave: |
Agropastoral systems; Brasil; Integração lavoura pecuária. |
|
Thesagro: |
Fungo; Micorriza; Solo. |
|
Thesaurus NAL: |
Acaulospora; Brazil; fungi; Glomus; Scutellospora; soil. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 01562naa a2200337 a 4500
001 1326835
005 2008-05-19
008 2006 bl uuuu u00u1 u #d
100 1 $aSILVA, S. V. da
245 $aFungos micorrízicos arbusculares em sistemas de integração lavoura e pecuária.
260 $c2006
300 $a1 p.$c1 CD-ROM.
500 $aCNPGC.
520 $aFungos micorrízicos arbusculares (FMAs) são simbiontes que aumentam a área de exploração efetiva das raízes, facilitando absorção de nutrientes essenciais pelas plantas. As comunidades de FMAs de um dado ecossistema podem ser avaliadas pela extração das estruturas de resistência que formam (esporos), que são distintos em cada espécie. Avaliou-se a diversidade de FMAs presentes num solo sob sistemas contrastantes de integração lavoura-pecuária, com o objetivo de avaliar os efeitos da exploração agropecuária na diversidade destes fungos, comparadas a uma mata original da área.
650 $aAcaulospora
650 $aBrazil
650 $afungi
650 $aGlomus
650 $aScutellospora
650 $asoil
650 $aFungo
650 $aMicorriza
650 $aSolo
653 $aAgropastoral systems
653 $aBrasil
653 $aIntegração lavoura pecuária
700 1 $aCAPRONI, A. L.
700 1 $aMIRANDA, C. H. B.
700 1 $aMACEDO, M. C. M.
700 1 $aFIGUEIRA, A. F.
773 $tIn: JORNADA CIENTÍFICA DA EMBRAPA GADO DE CORTE, 2., 2006, Campo Grande, MS. Anais [da]... 2. ed. Campo Grande, MS: Embrapa Gado de Corte, 2006.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Corte (CNPGC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
