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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
13/08/2019 |
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Data da última atualização: |
20/10/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, H. A. P. da; CAETANO, V. S.; PESSOA, D. D. V.; PACHECO, R. S.; ARAUJO, J. L. S. de. |
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Afiliação: |
Helder Anderson Pinto da Silva, UFRJ; Vanessa Santana Caetano, UFRRJ; Daniella Duarte Villarinho Pessoa, UFRJ; UFRRJ; JEAN LUIZ SIMOES DE ARAUJO, CNPAB. |
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Título: |
Molecular and biochemical changes of aging-induced nodules senescence in common bean. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Symbiosis, v. 79, p. 33-48, 2019. |
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DOI: |
https://doi.org/10.1007/s13199-019-00618-2 |
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Idioma: |
Inglês |
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Conteúdo: |
In the common bean (Phaseolus vulgaris L.), premature nodule senescence affects biological nitrogen fixation (BNF), reducing crop yield. Here, we investigated the molecular and biochemical changes in common bean nodules undergoing aging senescence. The experiments were carried out with three common bean genotypes and two nodule groups: senescent and non-senescent nodules. The expression patterns of 12 genes were assessed using RT-qPCR. In addition, global activities of ascorbate peroxidase (APX) and catalase (CAT), lipid peroxidation and total sugar content of nodules were also determined. Our results show that PvLb gene expression was down-regulated and PvGS(n-1) and PvUriII, genes involved in N metabolism, also decreased in senescent nodules. Transcripts related to the ethylene, abscisic acid and cytokinin phytohormones were up-regulated upon nodule senescence. Interestingly, PvSnakin-2 (SNAKIN-like cysteine rich protein), a gene related to plant-pathogen interaction, was induced in senescent nodules. The biochemical assays showed that in nodules undergoing senescence, APX activity was altered in only one genotype but CAT activity decreased in all common bean genotypes. Lipid peroxidation increased but total sugar content was not altered in nodules upon senescence. All these findings suggest that a delayed plant-response to Rhizobium infection is activated in common bean nodules undergoing aging senescence and this mechanism seems to be regulated by phytohormones and cell signaling as well, whereas the antioxidant defenses mediated by CAT are repressed, suggesting oxidative damage in nodules upon senescence. Our results offer insights into understanding nodule senescence metabolism and provide potential senescence markers for common bean nodules. MenosIn the common bean (Phaseolus vulgaris L.), premature nodule senescence affects biological nitrogen fixation (BNF), reducing crop yield. Here, we investigated the molecular and biochemical changes in common bean nodules undergoing aging senescence. The experiments were carried out with three common bean genotypes and two nodule groups: senescent and non-senescent nodules. The expression patterns of 12 genes were assessed using RT-qPCR. In addition, global activities of ascorbate peroxidase (APX) and catalase (CAT), lipid peroxidation and total sugar content of nodules were also determined. Our results show that PvLb gene expression was down-regulated and PvGS(n-1) and PvUriII, genes involved in N metabolism, also decreased in senescent nodules. Transcripts related to the ethylene, abscisic acid and cytokinin phytohormones were up-regulated upon nodule senescence. Interestingly, PvSnakin-2 (SNAKIN-like cysteine rich protein), a gene related to plant-pathogen interaction, was induced in senescent nodules. The biochemical assays showed that in nodules undergoing senescence, APX activity was altered in only one genotype but CAT activity decreased in all common bean genotypes. Lipid peroxidation increased but total sugar content was not altered in nodules upon senescence. All these findings suggest that a delayed plant-response to Rhizobium infection is activated in common bean nodules undergoing aging senescence and this mechanism seems to be regulated by phytohormones and cell ... Mostrar Tudo |
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Palavras-Chave: |
Biological nitrogen fixation; Nodule senescence; Root nodule RT-qPCR. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02458naa a2200217 a 4500
001 2111331
005 2021-10-20
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s13199-019-00618-2$2DOI
100 1 $aSILVA, H. A. P. da
245 $aMolecular and biochemical changes of aging-induced nodules senescence in common bean.$h[electronic resource]
260 $c2019
520 $aIn the common bean (Phaseolus vulgaris L.), premature nodule senescence affects biological nitrogen fixation (BNF), reducing crop yield. Here, we investigated the molecular and biochemical changes in common bean nodules undergoing aging senescence. The experiments were carried out with three common bean genotypes and two nodule groups: senescent and non-senescent nodules. The expression patterns of 12 genes were assessed using RT-qPCR. In addition, global activities of ascorbate peroxidase (APX) and catalase (CAT), lipid peroxidation and total sugar content of nodules were also determined. Our results show that PvLb gene expression was down-regulated and PvGS(n-1) and PvUriII, genes involved in N metabolism, also decreased in senescent nodules. Transcripts related to the ethylene, abscisic acid and cytokinin phytohormones were up-regulated upon nodule senescence. Interestingly, PvSnakin-2 (SNAKIN-like cysteine rich protein), a gene related to plant-pathogen interaction, was induced in senescent nodules. The biochemical assays showed that in nodules undergoing senescence, APX activity was altered in only one genotype but CAT activity decreased in all common bean genotypes. Lipid peroxidation increased but total sugar content was not altered in nodules upon senescence. All these findings suggest that a delayed plant-response to Rhizobium infection is activated in common bean nodules undergoing aging senescence and this mechanism seems to be regulated by phytohormones and cell signaling as well, whereas the antioxidant defenses mediated by CAT are repressed, suggesting oxidative damage in nodules upon senescence. Our results offer insights into understanding nodule senescence metabolism and provide potential senescence markers for common bean nodules.
653 $aBiological nitrogen fixation
653 $aNodule senescence
653 $aRoot nodule RT-qPCR
700 1 $aCAETANO, V. S.
700 1 $aPESSOA, D. D. V.
700 1 $aPACHECO, R. S.
700 1 $aARAUJO, J. L. S. de
773 $tSymbiosis$gv. 79, p. 33-48, 2019.
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